16S rRNA genes of gut microbiota from calves in response to florfenicol
(Gut microbiota of calves (16S rRNA profiling))

This data is NERC-funded but not held by the EIDC. This data is archived in the European Nucleotide Archive (ENA). This dataset contains sequences of 16S rRNA genes of gut microbiota of calves in response to preventive antibiotic therapy florfenicol by DNA-seq. The dataset was created from faecal samples (n=3) of the antibiotic treated animals over seven days and samples (n=3) of animals which have not been subjected to the antibiotic over the same time.

Publication date: 2020-10-05

Get the data

This record describes NERC-funded data managed by European Nucleotide Archive To access the data visit https://www.ebi.ac.uk/ena/browser/view/PRJEB33144

Provenance & quality

The 16S rRNA genes from these samples were amplified and sequenced. Total DNA samples were sequenced at three time points: before treatment and at T3 (day 3) and T7 (day 7) after treatment. The V3 and V4 regions of the bacterial 16S rRNA gene were amplified with standard Illumina primers, forward primer: 5’-CCTACGGGNGGCWGCAG-3’ and reverse primer: 5’-GACTACHVGGGTATCTAATCC-3’ to produce 550 bp products for sequencing (Vertis, Germany). Sequencing was carried out on an Illumina MiSeq, producing 2x300bp paired-end reads.

Supplemental information

This dataset is referenced in:

Dobrzanska D, Lamaudiere MTF, Rollason J, Acton L, Duncan M, Weedall GD, Morozov I. “Preventive Antibiotic Treatment of calves: Emergence of Dysbiosis Causing Propagation of Obese State Associated and Mobile Multidrug Resistance Carrying Bacteria”. Micro Biotech 2019, in press.

Correspondence/contact details

Igor Morozov
Coventry University

Other contacts

European Nucleotide Archive
European Nucleotide Archive

Additional metadata

Topic categories
Health , Farming
florfenicol,  preventive antibiotic treatment
Natural Environment Research Council Award: NE/N019288/1
Last updated
05 October 2020 15:54