A study on common carp gene expression response to cooling stress using Ribo-Seq

This data is NERC-funded but not held by the EIDC. This data is archived on the ArrayExpress repository (EBI).

Dataset contains Ribo-seq fastq data files for 83 common carp kidney samples as a function of time after cooling. The carp were held for over 3 months at 25°C and were randomly placed into two large aquarium tanks. One which remained throughout at 25°C (control) and the other was cooled over 3 days to 11°C for 23 days. Replicate specimens from both tanks were then killed at up to 17 timepoints after cooling and kidney tissue was sampled, and ribosomal footprints were quantified as a function of time after cooling using Ribo-Seq.



Publication date: 2020-10-29

Get the data

This record describes NERC-funded data managed by The European Bioinformatics Institute (EMBL-EBI) To access the data visit https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-8567/

Provenance & quality

Common carp were exposed to 23 days of reduced water temperature, and at timed intervals kidney tissues from replicated specimens were excised and stored at -80°C. RNA-seq libraries generated from ribosomal 'footprints' were prepared from each replicate animal, and sequenced using Illumina HiSeq4000.

Correspondence/contact details

Dr. Yongxiang Fang
University of Liverpool
 fangy@liverpool.ac.uk

Author

Fang, Y.
University of Liverpool

Other contacts

Custodian
Publisher

Additional metadata

Topic categories
Biota
Keywords
Cold stress,  Common carp,  Cyprinus carpio,  footprint analysis,  Ribo-seq
INSPIRE Theme
Environmental Monitoring Facilities
Last updated
29 October 2020 16:00