Transcriptomic responses of phytoplankton to acute and chronic thermal stress
(Thermal transcriptome)

This dataseries investigates the thermal transcriptome of phytoplankton in response to acute and chronic temperature stress. It is comprised of three subseries: 1. Thermal transcriptome of Phaeodactylum tricornutum in response to gradual heat stress. 2. Thermal transcriptome of Phaeodactylum tricornutum in steady-state growth, and 3. Thermal transcriptome of Synechocystis in response to gradual heat stress. To investigate the molecular underpinnings of gradual heat stress, we expose cultures to high temperature and follow changes in gene expression through time (over a 12 hour period) in comparison to optimal conditions. To understand how the thermal transcriptome response to chronic thermal stress, we acclimated P. tricornutum in steady state growth under optimal conditions and compare the gene expression to that under sub-optimal and supra-optimal conditions.

This data is NERC-funded but not held by the EIDC. This data is archived in the Gene Expression Omnibus (GEO) National Center Biotechnology Information (NCBI).

Get the data

This record describes NERC-funded data managed by Gene Expression Omnibus (GEO), National Center for Biotechnology Information (NCBI) To access the data visit https://www.ncbi.nlm.nih.gov/bioproject/PRJNA772171

Provenance & quality

The samples were collected and total RNA was extracted in the laboratories at the School of Life Sciences (Colchester Campus), University of Essex, U.K. For project ID LIMS19049 the total RNA was sent for library prepariton to the Exeter Sequencing Service at the University of Exeter (UK) and sequenced by the Centre for Genomic Research (CGR) at the University of Liverpool (UK). For projects LIMS20288 and LIMS23143 total RNA was sent to CGR Liverpool for both library preparation and sequencing. The raw data was downloaded onto the computing cluster at the University of Essex (UK) for downstream analysis.

Correspondence/contact details

Lawson, T.
University of Essex
 tlawson@essex.ac.uk

Other contacts

Custodian
Gene Expression Omnibus (GEO), National Center for Biotechnology Information (NCBI)
 tlawson@essex.ac.uk
Publisher
Gene Expression Omnibus (GEO), National Center for Biotechnology Information (NCBI)
 tlawson@essex.ac.uk

Additional metadata

Topic categories
Biota
Keywords
experimental manipulation,  heat shock,  photosynthesis,  Phytoplankton,  ramp,  thermal stress
Last updated
07 December 2021 14:44