Bombus terrestris parent of origin methylation analysis

Dataset contains DNA sequencing from reciprocal crosses of B.terrestris dalmatinus and B.terrestris audax which were carried out by Biobest, Leuven. Four successful colonies (one of each cross direction) from two ’families’ were housed at the University of Leuven and kept in 21◦C with red light conditions, they were fed ad libitum with pollen and a sugar syrup. Callow workers were tagged in order to determine age. Worker reproductive status was confirmed by ovary dissection and entire bodies were then stored at -80◦C along with the original queen mothers and male fathers. Three reproductive workers, aged 16-17days, were selected from queen-less conditions from each of the crosses.

This data is NERC-funded but not held by the EIDC. This data is archived in the NCBI SRA under BioProject PRJNA573820

Publication date: 2020-11-04

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This record describes NERC-funded data managed by NCBI Sequence Read Archive (SRA) To access the data visit https://www.ncbi.nlm.nih.gov/bioproject/PRJNA573820

Provenance & quality

DNA was extracted from whole heads of the mother and father of each colony as well as from 12 reproductive workers (three per colony) using the Qiagen DNeasyR Blood & Tissue Kit following the manufacturers protocol. Each sample was treated with RNAse. DNA from the three reproductive samples per colony were pooled to produce one representative offspring sample per colony. In summary, 12 samples were collected for sequencing – 4 * workers (3 heads per sample), 4 * queens (1 head) and 4 * males (1 head)

DNA quantity and quality were determined by Nanodrop and QubitR fluorometers as well as via gel electrophoresis. DNA was sent to BGI Tech Solution Co., Ltd.(Hong Kong) for sequencing using Illumina HiSeq 4000.

Sequencing data were checked using fastqc v.0.11.5 and poor quality bases were trimmed using cutadapt v.1.11 (Martin, 2011).

Correspondence/contact details

Dr. Benjamin Hunt
University of Exeter
 B.Hunt2@exeter.ac.uk

Author

Hunt, B.
University of Exeter

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Additional metadata

Topic categories
Biota
Keywords
Environmental survey
INSPIRE Theme
Environmental Monitoring Facilities
Last updated
21 April 2021 10:32