Pool-sequencing of 4 iso-Y guppy lines derived from the Paria population

This data is NERC-funded but not held by the EIDC. This data is archived in the European Nucleotide Archive (ENA) under accession number PRJEB36506. Guppy (Poecilia reticulata) colour traits have fascinated biologists for a hundred years, and present a unique system for investigating the evolution of colour polymorphism. The aim of this project is to identify regions of the guppy genome responsible for colour patterning in males of the species (the sex which shows colour patterning). Using pool-sequencing, we genotyped four inbred guppy lines (n=48 per line), which originate from a natural guppy population, Paria. Each line was founded by one male showing distinct colouration patterns, and has been backcrossed with unrelated females every generation for ~40 generations, allowing Y-linked colour to be inherited intact from father to son (iso-Y lines).

Provenance & quality

DNA was extracted from 48 individuals for each iso-Y line. DNA quality was assessed using gel electrophoresis and DNA quality and concentration. Individuals from each line were pooled at equal concentrations to create pool-sequencing libraries for sequencing. Library preparation and sequencing was performed at the University of Exeter Sequencing Service, UK. To obtain an optimal coverage of >=100x, the four iso-Y line libraries were sequenced across seven lanes on a HiSeq 2500 in standard mode, with a 125bp paired-end metric. Quality control of the sequencing data was undertaken following standard pipelines (FastQC, qualimap).

Correspondence/contact details

Fraser, B.
University of Exeter
 B.Fraser@exeter.ac.uk

Other contacts

Custodian
European Nucleotide Archive
 enquiries@ceh.ac.uk
Publisher
European Nucleotide Archive
 enquiries@ceh.ac.uk

Additional metadata

Topic categories
Biota
Keywords
colour traits,  guppy,  pool-sequencing,  Y-linkage
Last updated
04 November 2020 10:26