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Sites were located near the villages of Obergurgl (lat., long. = 46.844833, 11.023783; mean elevation = 2279m), Soelden (46.978367, 10.972217, mean elevation = 2469m) and Vent (46.863217, 10.896800, mean elevation = 2472m).\n\nSoil microbial data include phospholipid fatty acid (PLFA) analyses, and bacterial (16S Small subunit ribosomal RNA) and fungal (internal transcribed spacer region 2) high throughput sequences. Soil biogeochemical data include soil extracellular enzyme activities, soil pH, gravimetric moisture content and various C and N pools and fluxes.\n\nThe experiment was funded by NERC project NE/N009452/1.","distributionFormats":[{"name":"Comma-separated values (CSV)","type":"text/csv","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"funding":[{"funderName":"Natural Environment Research Council","funderIdentifier":"https://ror.org/02b5d8509","awardTitle":"Soil microbial community dynamics and biogeochemical cycles under global change: effects of climate and vegetation change in alpine ecosystems","awardNumber":"NE/N009452/1","awardURI":"https://gtr.ukri.org/projects?ref=NE/N009452/1","orcid":false,"ror":true}],"id":"977c422d-8529-432d-85d7-5de3c8dfdc5d","incomingCitationCount":0,"infoLinks":[{"url":"https://data-package.ceh.ac.uk/sd/977c422d-8529-432d-85d7-5de3c8dfdc5d.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"inspireThemes":[{"theme":"Soil","uri":"http://inspire.ec.europa.eu/theme/so"}],"keywordsOther":[{"value":"Ecosystem functions"},{"value":"alpine"},{"value":"Obergurgl","uri":"http://sws.geonames.org/2770087"},{"value":"Soil microbiome"},{"value":"Soelden ","uri":"http://sws.geonames.org/7872529"},{"value":"Vent","uri":"http://sws.geonames.org/2762441"},{"value":"dissolved organic nitrogen","uri":"http://onto.nerc.ac.uk/CAST/284"},{"value":"Microbial biomass"},{"value":"Enzyme activity"},{"value":"Urease activity"},{"value":"Phospholipid-derived fatty acids (PLFA)"},{"value":"Gravimetric soil water content"},{"value":"carbon","uri":"http://www.eionet.europa.eu/gemet/concept/1160"},{"value":"nitrogen","uri":"http://www.eionet.europa.eu/gemet/concept/5605"},{"value":"ammonium","uri":"http://www.eionet.europa.eu/gemet/concept/379"},{"value":"nitrate","uri":"http://www.eionet.europa.eu/gemet/concept/5593"},{"value":"soil chemistry","uri":"http://www.eionet.europa.eu/gemet/concept/7849"},{"value":"biomass","uri":"http://www.eionet.europa.eu/gemet/concept/883"},{"value":"microbial resource","uri":"http://www.eionet.europa.eu/gemet/concept/5215"},{"value":"soil water","uri":"http://www.eionet.europa.eu/gemet/concept/7902"},{"value":"Austria","uri":"http://sws.geonames.org/2782113"}],"keywordsTheme":[{"value":"Soil","uri":"http://onto.nerc.ac.uk/CEHMD/topic/17"},{"value":"Climate and climate change","uri":"http://onto.nerc.ac.uk/CEHMD/topic/4"}],"licences":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}],"lineage":"All data were collected by the authors according to standardised protocols. Soil was sampled on 22nd - 24th July 2019 by taking soil cores (Ø = 2 cm, depth = 10 cm) using a steel corer from five random locations in each plot (plot size = 2m x 2m). Soil cores from the same plot were pooled and homogenised, any vegetation or litter was separated and discarded. Sub-samples (approx. 200 mg) were directly taken and lysed in the field for molecular analyses. Soil samples were sieved (4 mm), stored at 4°C for up to 2 weeks, and shipped to The University of Manchester (UK) Soil and Ecosystem Ecology Lab for extracellular soil enzyme and biogeochemical analyses. Lysed soil samples for molecular analyses were stored at -80°C and shipped to UKCEH Wallingford for DNA extraction and sequencing. We ensured all measured values fit within those of calibration standards. All calculations were checked for errors before data was accepted and data were checked for any anomalies.","metadataDate":"2026-05-11T18:08:53","notGEMINI":false,"onlineResources":[{"url":"https://data-package.ceh.ac.uk/data/977c422d-8529-432d-85d7-5de3c8dfdc5d","name":"Download the data","description":"Download a copy of this data","function":"download","type":"OTHER"},{"url":"https://data-package.ceh.ac.uk/sd/977c422d-8529-432d-85d7-5de3c8dfdc5d.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"pointsOfContact":[{"familyName":"Broadbent","givenName":"A.A.D.","organisationName":"The University of Manchester","role":"pointOfContact","email":"arthur.broadbent@manchester.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-8438-7163","fullName":"Broadbent, A.A.D.","pointOfContact":"The University of 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