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dcterms:title "Alpine grassland soil microbial and biogeochemical data from a climate change and shrub expansion experiment in Tyrol, Austria, July 2019" ;
dcterms:identifier "https://catalogue.ceh.ac.uk/id/977c422d-8529-432d-85d7-5de3c8dfdc5d","https://doi.org/10.5285/977c422d-8529-432d-85d7-5de3c8dfdc5d" ;
dcterms:bibliographicCitation "Broadbent, A.A.D., Bahn, M., Pritchard, W.J., Newbold, L., Goodall, T., Guinta, A., Snell, H.S.K., Cordero, I., Michas, A., Grant, H.K., Soto, D.X., Kaufmann, R., Schloter, M., Griffiths, R.I., Bardgett, R.D. (2021). Alpine grassland soil microbial and biogeochemical data from a climate change and shrub expansion experiment in Tyrol, Austria, July 2019. NERC Environmental Information Data Centre. https://doi.org/10.5285/977c422d-8529-432d-85d7-5de3c8dfdc5d" ;
dcterms:description "Data comprise soil microbial and biogeochemical data collected during a climate and vegetation change experiment conducted across three valleys in Tyrol, in the Austrian Alps. Sites were located near the villages of Obergurgl (lat., long. = 46.844833, 11.023783; mean elevation = 2279m), Soelden (46.978367, 10.972217, mean elevation = 2469m) and Vent (46.863217, 10.896800, mean elevation = 2472m). Soil microbial data include phospholipid fatty acid (PLFA) analyses, and bacterial (16S Small subunit ribosomal RNA) and fungal (internal transcribed spacer region 2) high throughput sequences. Soil biogeochemical data include soil extracellular enzyme activities, soil pH, gravimetric moisture content and various C and N pools and fluxes. The experiment was funded by NERC project NE/N009452/1." ;
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rdfs:label "All data were collected by the authors according to standardised protocols. Soil was sampled on 22nd - 24th July 2019 by taking soil cores (Ø = 2 cm, depth = 10 cm) using a steel corer from five random locations in each plot (plot size = 2m x 2m). Soil cores from the same plot were pooled and homogenised, any vegetation or litter was separated and discarded. Sub-samples (approx. 200 mg) were directly taken and lysed in the field for molecular analyses. Soil samples were sieved (4 mm), stored at 4°C for up to 2 weeks, and shipped to The University of Manchester (UK) Soil and Ecosystem Ecology Lab for extracellular soil enzyme and biogeochemical analyses. Lysed soil samples for molecular analyses were stored at -80°C and shipped to UKCEH Wallingford for DNA extraction and sequencing. We ensured all measured values fit within those of calibration standards. All calculations were checked for errors before data was accepted and data were checked for any anomalies."
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