PREFIX rdf: PREFIX rdfs: PREFIX skos: PREFIX owl: PREFIX xsd: PREFIX dcterms: PREFIX dcat: PREFIX dcmitype: PREFIX foaf: PREFIX geo: PREFIX vcard: PREFIX prov: PREFIX odrs: PREFIX sosa: PREFIX sdo: PREFIX ef: PREFIX doo: PREFIX adms: PREFIX : :9d0a6437-f01e-4cf3-9f83-86d2f4ead869 dcterms:title "Concentrations and antibiotic resistance of faecal indicator organisms and human pathogens associated with sewage waste on Scottish beaches, 2021" ; dcterms:identifier ; adms:identifier ; dcterms:bibliographicCitation "Metcalf, R., White, H.L., Moresco, V., Ormsby, M.J., Oliver, D.O., Quilliam, R. (2024). Concentrations and antibiotic resistance of faecal indicator organisms and human pathogens associated with sewage waste on Scottish beaches, 2021. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/9d0a6437-f01e-4cf3-9f83-86d2f4ead869" ; dcterms:description "The data includes concentrations of faecal indicator organisms isolated from the plastic waste and natural substrates found on Scottish beaches, as well as the antibiotic resistance of selected strains of these organisms. Sewage-associated plastic waste, and naturally occurring substrates (seaweed and sand), were collected in 2021 from ten beaches along the Firth of Forth estuary (Scotland, UK). The concentrations of faecal indicator organisms (FIOs), E. coli and intestinal enterococci (IE), and potential human pathogens (Vibrio spp.) on the plastic waste and natural substrates were analysed using selective media. Minimum inhibitory concentration (MIC) analysis was used to determine antibiotic resistance in selected strains." ; dcterms:provenance [ a dcterms:ProvenanceStatement ; rdfs:label "Sewage-associated plastic waste (wipes, cotton bud sticks and sanitary products) was collected from ten beaches. All samples were stored at 4 °C and processed within 48 h. Replicate composite samples of wipes, cotton bud sticks and seaweed from each site were pre-enriched in non-selective media Luria-Bertani (LB) broth or Vibrio selective Bile Peptone broth at 37 °C in a shaking incubator for 18 h. Following pre-enrichment, serial dilutions were carried out in phosphate buffered saline (PBS) and dilutions were plated onto selective media. All plates were inverted and incubated. The presence or absence of target bacteria on all sewage-associated plastic waste samples was determined by enumerating colony forming units (CFUs). Minimum inhibitory concentration (MIC): Representative individual colonies of E. coli, IE and Vibrio spp. isolated from seaweed, wet wipe, and cotton bud stick samples, were picked off agar plates, grown further overnight in LB broth, and subjected to MIC analysis to determine antibiotic resistance to amoxicillin, ampicillin, streptomycin, cephalexin, and tetracycline at varying concentrations. Two-fold serial dilutions of each antibiotic were made in LB broth in 96-well plates. Bacterial cultures were adjusted to an optical density at a wavelength of 600nm (OD600nm) of 0.1, and 10 μL added to each well of a 96-well plate. Samples were incubated overnight at 37 °C." ] ; dcterms:spatial [ a dcterms:Location ; dcat:bbox "POLYGON((-3.878 55.848, -3.878 56.277, -2.747 56.277, -2.747 55.848, -3.878 55.848))"^^geo:wktLiteral ; ] ; dcterms:temporal [ a dcterms:PeriodOfTime ; dcat:startDate "2021-12-01"^^xsd:date ; dcat:endDate "2021-12-31"^^xsd:date ; ] ; dcat:contactPoint ; dcterms:publisher ; dcterms:isPartOf ; dcterms:relation ; dcterms:subject "Beach pollution", "Environmental pathogens", "Plastic pollution", "Plastisphere", "Sewage discharge", "Wet wipes" ; a dcat:Dataset; dcterms:type dcmitype:Dataset ; dcterms:available "2024-08-09"^^xsd:date ; dcat:landingPage , ; dcterms:license ; dcterms:accessRights ; dcat:distribution [ dcat:accessURL ; dcterms:license ; dcterms:accessRights ; dcterms:format [ a dcterms:IMT ; rdf:value "Comma-separated values (CSV)" ; rdfs:label "Comma-separated values (CSV)" ] ; ]; dcterms:creator , :9d0a6437-f01e-4cf3-9f83-86d2f4ead869_a1, :9d0a6437-f01e-4cf3-9f83-86d2f4ead869_a2, :9d0a6437-f01e-4cf3-9f83-86d2f4ead869_a3, :9d0a6437-f01e-4cf3-9f83-86d2f4ead869_a4, ; dcterms:language . a foaf:Person ; foaf:name "Metcalf, R." ; foaf:familyName "Metcalf" ; foaf:givenName "R." ; vcard:hasEmail "Rebecca.metcalf@stir.ac.uk" ; foaf:member [foaf:name "University of Stirling"] ; . a foaf:Organization ; foaf:name "NERC EDS Environmental Information Data Centre" ; vcard:hasEmail "info@eidc.ac.uk" ; foaf:member ; . a foaf:Person ; foaf:name "Metcalf, R." ; foaf:familyName "Metcalf" ; foaf:givenName "R." ; vcard:hasEmail "Rebecca.metcalf@stir.ac.uk" ; foaf:member [foaf:name "University of Stirling"] ; . :9d0a6437-f01e-4cf3-9f83-86d2f4ead869_a1 a foaf:Person ; foaf:name "White, H.L." ; foaf:familyName "White" ; foaf:givenName "H.L." ; vcard:hasEmail "Hannah.white@stir.ac.uk" ; foaf:member [foaf:name "University of Stirling"] ; . :9d0a6437-f01e-4cf3-9f83-86d2f4ead869_a2 a foaf:Person ; foaf:name "Moresco, V." ; foaf:familyName "Moresco" ; foaf:givenName "V." ; vcard:hasEmail "van.moresco@gmail.com" ; foaf:member [foaf:name "University of California Riverside"] ; . :9d0a6437-f01e-4cf3-9f83-86d2f4ead869_a3 a foaf:Person ; foaf:name "Ormsby, M.J." ; foaf:familyName "Ormsby" ; foaf:givenName "M.J." ; vcard:hasEmail "Michael.ormsby1@stir.ac.uk" ; foaf:member [foaf:name "University of Stirling"] ; . :9d0a6437-f01e-4cf3-9f83-86d2f4ead869_a4 a foaf:Person ; foaf:name "Oliver, D.O." ; foaf:familyName "Oliver" ; foaf:givenName "D.O." ; vcard:hasEmail "David.oliver@stir.ac.uk" ; foaf:member [foaf:name "University of Stirling"] ; . a foaf:Person ; foaf:name "Quilliam, R." ; foaf:familyName "Quilliam" ; foaf:givenName "R." ; vcard:hasEmail "Richard.quilliam@stir.ac.uk" ; foaf:member [foaf:name "University of Stirling"] ; .