A study on gene expression responses in the common carp to cooling stress using RNA-seq

This data is NERC-funded but not held by the EIDC. This data is archived on the ArrayExpress repository (EBI).

Dataset contains RNA-seq fastq data files for 83 common carp kidney samples as a function of time after cooling. The carp were held in two large aquarium tanks, one which remained throughout at 25°C (control) and the other that was cooled over 3 days to 11°C and held for 23 days. Replicate specimens from both tanks were then killed at up to 17 timepoints after cooling, kidney tissue was sampled, and transcript expression as a function of time after cooling determined by RNA-seq.

Publication date: 2020-10-29

Get the data

This record describes NERC-funded data managed by The European Bioinformatics Institute (EMBL-EBI) To access the data visit https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-8458

Provenance & quality

Common carp were exposed to 23 days of reduced water temperature, and at timed intervals kidney tissues from replicated specimens were excised and stored at -80°C. RNA-seq libraries were prepared from mRNAs isolated from each replicate, and sequenced using Illumina HiSeq4000.

Correspondence/contact details

Dr. Yongxiang Fang
University of Liverpool
 fangy@liverpool.ac.uk

Author

Fang, Y.
University of Liverpool

Other contacts

Custodian
Publisher

Additional metadata

Topic categories
Biota
Keywords
Cold stress,  Common carp,  Cyprinus carpio,  RNA-seq,  Transcript analysis
INSPIRE Theme
Environmental Monitoring Facilities
Funding
Natural Environment Research Council
Last updated
04 November 2020 16:14