Soil bacterial and fungal communities from the Climoor long term climate change experiment in Clocaenog forest, Wales, UK (2003 and 2017)
(Soil 16S and ITS2 sequences from the Clocaenog climate change experiment)

This data is NERC-funded but not held by the EIDC. The data is archived in European Nucleotide Archive under primary accession reference PRJEB33721. This dataset is the DNA sequences from Illumina MiSeq sequencing of the bacterial 16S and fungal ITS2 genes in climate manipulated soils from the Climoor field experiment. Soil samples were collected in 2003 and 2017 after 4 and 18 years of manipulation, respectively.
The experimental field site consists of three untreated control plots, three plots where the plant canopy air is artificially warmed during night time hours, and three plots where rainfall is excluded from the plots at least during the plants' growing season (March to September). The Climoor field experiment intends to answer questions regarding the effects of warming and drought on ecosystem processes and has been running since 1999. The microbial community data aims to understand how changes in soil hydrological and chemical properties have influenced the soil microbial composition and the implications of this for biogeochemical cycling. The data were collected as part of NERC project NEC05670 CWI-STU Fiona Seaton as part of the NERC Soils Training And Research Studentships Centre for Doctoral Training (STARS)

Publication date: 2021-03-15

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Study area
Temporal extent
2013-02-15    to    2017-02-15

Provenance & quality

Soil samples were collected in February 2003 and February 2017 respectively by students and staff at the Climoor field site. They were stored at -20 degrees Celsius in CEH Bangor until transported to and sequenced at CEH Wallingford. A small portion of soil (approximately 0.2gramme) was taken from each sample and the DNA extracted using a MOBIO PowerSoil® extraction kit. The 16S genes were amplified using primers for the V4 region (primers 515F-806R, as in the Earth Microbiome Project) and the ITS2 genes amplified using primers from Ihrmark et al (FEMS Microbiol Ecol 82 (2012) 666–677). The DNA was sequenced upon an Illumina MiSeq system. Samples with no sequences found were removed before uploading.

Correspondence/contact details

Seaton, F.M.
UK Centre for Ecology & Hydrology


Seaton, F.M.
UK Centre for Ecology & Hydrology

Other contacts

The European Bioinformatics Institute (EMBL-EBI)
The European Bioinformatics Institute (EMBL-EBI)

Additional metadata

Topic categories
bacterial,  Climate change,  Climoor,  Clocaenog,  DNA,  fungal,  Sequencing,  Soil
Environmental Monitoring Facilities
Natural Environment Research Council Award: NEC05670 CWI-STU Fiona Seaton
Last updated
22 March 2021 16:57