Linking potential greenhouse gas and NO fluxes to soil microbial communities in incubation experiments with soil from the SAFE landscape
This dataset is embargoed and will be made available by 30 June 2020 at the latest
This record describes NERC-funded data managed by Zenodo
To access the data visit https://doi.org/10.5281/zenodo.3251901
These data are NERC-funded but not held by the EIDC. These data are archived in the SAFE repository, hosted by Zenodo.
This dataset contains results from a laboratory study that was carried out with soil taken from logged tropical forests and nearby oil palm plantations of different ages in Malaysian Borneo, Sabah within the SAFE project landscape. The soils were from: Logged Fragmented Forest (LFE) pH 6, Fragment E (FE) pH 6, ~7 year old oil palm plantation (OP7) pH 4.5 , small riparian area below OP7 (RR), pH 5.8, ~2 year old row of oil palm (OP2) pH 4.5. The soils were collected in Nov 2016, from the top 0-10 cm. During the laboratory incubation all cores were sampled for greenhouse gases (methane, nitrous oxide and carbon dioxide) as well as nitric oxide (NO). Other environmental parameters were measured at the same time as possible explanatory variables for correlation with recorded greenhouse gas and nitric oxide fluxes including soil and air temperature, soil moisture, soil mineral N (nitrate and ammonium) and gene transcript abundance.
Provenance & quality
The data were collected using a laboratory version of the static chamber method. Gas samples were taken from incubation tubes via syringe and injected immediately into a gas chromatagraph (GC) in the lab. Concentrations were calculated from GC peak areas, calibrated against standard gases of known concentration. Fluxes were calculated from the concentration change in each chamber by linear regression. Fluxes are expressed as microgramme per g of dry soil per hour for CH4 and N2O and as milligramme per g of dry soil per hour for CO2. NO was measured by Chemoluminescense in situ and expressed as a flux of nanogramme per g of dry soil. For soil microbial analysis, RT-qPCR of targeted marker gene transcripts was used and results expressed as gene abundance.
For the experiment 200 g of dry soil was placed into 5 cm diameter Perspex tubes (each landuse in triplicate) and incubated at 25 degrees C. An ammonium nitrate (NH4NO3) solution was applied to simulate N deposition of approx. 5 kg N ha-1 y-1 and applied to all cores. In addition, ammonium nitrate solution to simulate N fertilisation of 50 kg N ha-1 y-1 was then again applied to OP2, OP7, RR. Soil moisture was kept constant. Subsamples were taken from identical 'clone' cores for pH, KCl extractable N (NH4, NO3) and microbial analysis.
Other useful information regarding this dataset:
Climatology / Meteorology / Atmosphere
logged tropical forest,
soil microbial activity,
Natural Environment Research Council Award: NE/K016091/1
10 January 2020 11:28