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Inbreeding was minimized by avoiding crossing family lines. Traits that were measured include adult body mass, development time, and lifespan. The data were collected in 2023 by researchers from the University of Glasgow to investigate the effects of parental age and temperature conditions on offspring life-history. We chose this species because we have complementary field projects on field crickets and they are easily maintained in a lab. \n\n","documentType":"GEMINI_DOCUMENT","familyName":["Pitt","Sheen","O'Connor","Boonekamp"],"fullName":["Jelle Boonekamp","Pitt, M.","Sheen, T.","O'Connor, B.","Boonekamp, J."],"funder":["Natural Environment Research Council"],"givenName":["Mark","Tim","Brendan","Jelle"],"grant":["NE/X012018/1"],"identifier":"085ec3ff-110d-4dbf-96da-7418ab9a7c5a","incomingCitationCount":0,"keyword":["Animal behaviour","evolution","field crickets"],"keywordsOther":["evolution","field crickets"],"keywordsTheme":["Animal behaviour"],"licence":"Open government licence","lineage":"Crickets were housed in individual boxes (approximately 18x10x7cm) from hatching . All individuals were kept at 28 degrees Celsius. Development time and lifespan data were obtained by weekly survival and adult emergence checks. Body mass was recorded to the nearest microgram using a calibrated balance. Weakly checks were done to record the date of adult emergence or death. Individuals had access to food (dried grass pellets - rabbit food from Science Selective) and water ad libitum. The data were entered in an Access data base and subsequently exported as csv file.","metadataDate":"2026-06-29T18:25:31.000Z","organisation":["University of Glasgow","NERC EDS Environmental Information Data Centre"],"publicationDate":"2025-06-25T00:00:00.000Z","recordType":"Dataset","resourceIdentifier":["https://catalogue.ceh.ac.uk/id/085ec3ff-110d-4dbf-96da-7418ab9a7c5a","10.5285/085ec3ff-110d-4dbf-96da-7418ab9a7c5a","doi:10.5285/085ec3ff-110d-4dbf-96da-7418ab9a7c5a"],"resourceType":"Non-geographic dataset","rightsHolder":["University of Glasgow"],"ror":["https://ror.org/00vtgdb53","https://ror.org/04xw4m193"],"shortenedDescription":"The data comprises records of phenotypic traits of outbred Gryllus bimaculatus that were bred in a laboratory. Inbreeding was minimized by avoiding crossing family lines. Traits that were measured include adult body mass, development time, and lifespan. The data were…","state":"published","title":"Phenotypic traits of laboratory-housed field crickets (Gryllus bimaculatus) bred from parents with a range of ages","topic":["0/Animal behaviour/"],"version":1.0,"view":["public","katwri"]},{"authorAffiliation":["Centre for Ecology & Hydrology","Rothamsted Research","Bangor University"],"authorFamilyName":["Goodall","Griffiths","Blaud","Abadie","Clark","Hirsch","Carswell","Misselbrook","Sánchez-Rodríguez","Chadwick","Jones","Reinsch"],"authorFullName":["Goodall, T.I.","Griffiths, R.I.","Blaud, A.","Abadie, M.","Clark, I.M.","Hirsch, P.R.","Carswell, A.M.","Misselbrook, T.H.","Sánchez-Rodríguez, A.R.","Chadwick, D.R.","Jones, D.L.","Reinsch, S."],"authorGivenName":["T.I.","R.I.","A","M.","I.M.","P.R.","A.M.","T.H.","A.R.","D.R.","D.L.","S."],"authorOrcid":["https://orcid.org/0000-0003-0860-8080","https://orcid.org/0000-0002-1589-5420","https://orcid.org/0000-0003-0580-6698","https://orcid.org/0000-0002-4594-3606","https://orcid.org/0000-0001-8734-2035","https://orcid.org/0000-0002-1482-4209","https://orcid.org/0000-0003-4649-0677"],"authorRor":["https://ror.org/00pggkr55","https://ror.org/0347fy350","https://ror.org/006jb1a24"],"availability":"Available","catalogue":"eidc","dataFormat":["Comma-separated values (CSV)"],"description":"The data consist of nitrogen gene data, soil biodiversity indices and microbial community composition for three soil depths (0-15, 15-30 and 30-60 cm) from a winter wheat field experiment located in the United Kingdom and collected between April 2017 and August 2017. The sites were Rothamsted Research at North Wyke in Devon  and Bangor University at Henfaes Research Station in North Wales.\n\nAt each site measurements were taken from 15 plots, organised within a randomised complete block design where 5 plots did not receive fertilizers (controls), 5 plots received food-based digestate, and 5 plots received acidified food based digestate a nitrification inhibitor. Soil samples were taken within two weeks of digestate application and shortly before winter wheat harvest.\n\nSoil chemical parameters were: soil nitrate, ammonium, dissolved organic carbon and nitrogen, amino acids and peptides, soil organic matter content as loss-on-ignition, pH, sodium, potassium, calcium, magnesium, permanganate oxdisable carbon citric acid extractable phosphorous, Olsen-P and total carbon, nitrogen and phosphorus. Soil biological measure were: microbial biomass carbon and nitrogen. \n\nSoil samples were taken by members of staff from Centre of Ecology & Hydrology (Bangor), Bangor University, School of Environment, Natural Resources & Geography Sustainable Agricultural Sciences, and Rothamsted Research North Wyke. Measurements were carried out Rothamsted Research Harpenden and the Centre of Ecology & Hydrology (Wallingford). Soil physico-chemical parameters were measured on the same soil samples and are presented in a related dataset. https://catalogue.ceh.ac.uk/id/90df9dfa-a0c8-4ead-a13d-0a0a13cda7ab\n\nData was collected for the Newton Fund project “UK-China Virtual Joint Centre for Improved Nitrogen Agronomy”. Funded by Biotechnology and Biological Sciences Research Council (BBSRC) and NERC -  Ref BB/N013468/1","documentType":"GEMINI_DOCUMENT","familyName":["Reinsch","Goodall","Griffiths","Blaud","Abadie","Clark","Hirsch","Carswell","Misselbrook","Sánchez-Rodríguez","Chadwick","Jones"],"fullName":["Reinsch, S.","Goodall, T.I.","Griffiths, R.I.","Blaud, A.","Abadie, M.","Clark, I.M.","Hirsch, P.R.","Carswell, A.M.","Misselbrook, T.H.","Sánchez-Rodríguez, A.R.","Chadwick, D.R.","Jones, D.L."],"funder":["Biotechnology and Biological Sciences Research Council"],"givenName":["S.","T.I.","R.I.","A","M.","I.M.","P.R.","A.M.","T.H.","A.R.","D.R.","D.L."],"grant":["BB/N013468/1"],"identifier":"391c0294-07f1-4856-b592-428bd44055ca","incomingCitationCount":0,"keyword":["winter wheat","nitrogen","fertiliser","digestate","operational taxonomic unit","diversity index","bacterial community composition"],"keywordsOther":["winter wheat","nitrogen","fertiliser","digestate","operational taxonomic unit","diversity index","bacterial community composition"],"licence":"Open government licence","lineage":"Soil samples were taken by members of staff from the different sites. Samples were sent to Rothamsted Research Harpenden for DNA extractions and extracts were analysed for nitrogen genes (nifH, amoA, nirK, nirS, nosZ, ureC). Extracts were also sent to CEH Wallingford for analyses of operational taxonomic units (OTUs').\n\nSoil community DNA was extracted from 0.25 grams soil using the MoBio DNA PowerSoil DNA isolation kit (Mo Bio Laboratories, Inc. Carlsbad, CA), following manufacturer’s protocol. Extracted DNA was quantified by fluorometer Qubit® 2.0 dsDNA BR Assay Kit (Thermo Fisher Scientific) and quality checked by nanodrop (Thermo Fisher Scientific).\n\nQuantitative-Polymerase chain reaction (qPCR) protocols were followed as detailed in De Sosa et al. 2018.\n\nExtracted DNA was supplied from Rothamsted Research in Harpenden for amplicon sequencing at the facilities of the Molecular Ecology Group, Centre for Ecology & Hydrology, Wallingford. <50 μL volumes of DNA extract were supplied in 96-well plate format, these were subject to 16S Ribosomal ribonucleic acid (rRNA) amplicon sequencing as described in Kozich 2013 and Internal transcribed spacer (ITS) rRNA amplicon sequencing employing the Kozich 2013 strategy and Ihrmark 2012.\n\nAll results were entered into a Microsoft Excel spreadsheet and converted to .csv files for ingestion into the Environmental Information Data Centre.","locations":["POLYGON((-4.022 50.767, -4.022 53.241, -3.892 53.241, -3.892 50.767, -4.022 50.767))"],"metadataDate":"2026-06-29T18:13:31.000Z","orcid":["https://orcid.org/0000-0003-4649-0677","https://orcid.org/0000-0003-0860-8080","https://orcid.org/0000-0002-1589-5420","https://orcid.org/0000-0003-0580-6698","https://orcid.org/0000-0002-4594-3606","https://orcid.org/0000-0001-8734-2035","https://orcid.org/0000-0002-1482-4209"],"organisation":["UK Centre for Ecology & Hydrology","Centre for Ecology & Hydrology","Rothamsted Research","Bangor University","NERC EDS Environmental Information Data Centre","NERC Environmental Information Data Centre"],"publicationDate":"2019-04-23T00:00:00.000Z","recordType":"Dataset","resourceIdentifier":["https://catalogue.ceh.ac.uk/id/391c0294-07f1-4856-b592-428bd44055ca","10.5285/391c0294-07f1-4856-b592-428bd44055ca","doi:10.5285/391c0294-07f1-4856-b592-428bd44055ca"],"resourceType":"Dataset","ror":["https://ror.org/00pggkr55","https://ror.org/0347fy350","https://ror.org/006jb1a24","https://ror.org/04xw4m193"],"shortenedDescription":"The data consist of nitrogen gene data, soil biodiversity indices and microbial community composition for three soil depths (0-15, 15-30 and 30-60 cm) from a winter wheat field experiment located in the United Kingdom and collected between April 2017 and August 2017.…","state":"published","supplementalDescription":["Kozich, J.J., Westcott, S.L., Baxter, N.T., Highlander, S. K., & Schloss, P.D. (2013). Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform. Applied and Environmental Microbiology, 79(17), 5112–5120.","de Sosa, L.L., Glanville, H.C., Marshall, M.R., Schnepf, A., Cooper, D.M., Hill, P.W., … Jones, D.L. (2018). Stoichiometric constraints on the microbial processing of carbon with soil depth along a riparian hillslope. Biology and Fertility of Soils, 54(8), 949–963."],"supplementalName":["The UK-China Virtual Joint Centre for Improved Nitrogen Agronomy (CINAG)"],"title":"Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017)","version":1.0,"view":["public","danor"]},{"authorAffiliation":["Centre for Ecology & Hydrology","Bangor University","Rothamsted Research"],"authorFamilyName":["Reinsch","Lebron","Sánchez-Rodríguez","Carswell","Carter","Chadwick","Cotton","Guyatt","Harvey","Hunt","Jones","Keenan","Lawlor","Marshall","Misselbrook","Patel","Pinder","Pereira","Saunders","Tanna","Thompson","Emmett"],"authorFullName":["Reinsch, S.","Lebron, I.","Sánchez-Rodríguez, A.R.","Carswell, A.M.","Carter, H.","Chadwick, D.R.","Cotton, J.M.","Guyatt, H.","Harvey, R.","Hunt, A.","Jones, D.L.","Keenan, P.O.","Lawlor, A.J.","Marshall, M.R.","Misselbrook, T.H.","Patel, M.","Pinder, A.P.","Pereira, M.G.","Saunders, K.S.","Tanna, B.","Thompson, N.","Emmett, B.A."],"authorGivenName":["S.","I.","A.R.","A.M.","H.","D.R.","J.M.","R.","A.","D.L.","P.O.","A.J.","M.R.","T.H.","M.","A.P.","M.G.","K.S.","B.","N.","B.A."],"authorOrcid":["https://orcid.org/0000-0003-4649-0677","https://orcid.org/0000-0001-8610-9717","https://orcid.org/0000-0001-8734-2035","https://orcid.org/0000-0003-0580-6698","https://orcid.org/0000-0002-1482-4209","https://orcid.org/0000-0003-2869-0616","https://orcid.org/0000-0002-4594-3606","https://orcid.org/0000-0002-2713-4389"],"authorRor":["https://ror.org/00pggkr55","https://ror.org/006jb1a24","https://ror.org/051h34p92"],"availability":"Available","catalogue":"eidc","dataFormat":["Comma-separated values (CSV)"],"description":"The data consist of soil physicochemical and biological data for three soil depths (0-15, 15-30 and 30-60 cm) from a winter wheat field experiment located at two research sites in the United Kingdom. Soil samples were collected between April 2017 and August 2017. Extractions and measurements were carried out thereafter. The sites were Rothamsted Research at North Wyke in Devon and Bangor University at Henfaes Research Station in North Wales.\n\nAt each site measurements were taken from 15 plots, organised within a randomised complete block design where 5 plots did not receive fertilizers (controls), 5 plots received food-based digestate, and 5 plots received acidified food based digestate and the nitrification inhibitor 3,4-dimethylpyrazole (DMPP; ADNI). Soil samples were taken within 2 weeks of digestate application and shortly before winter wheat harvest.\n\nMicrobial community composition and nitrogen genes were measured on the same soil samples and are presented in a separate dataset (https://catalogue.ceh.ac.uk/id/391c0294-07f1-4856-b592-428bd44055ca).\n\nSoil samples were taken by members of staff from Centre of Ecology & Hydrology (Bangor), Bangor University, School of Environment, Natural Resources & Geography Sustainable Agricultural Sciences, and Rothamsted Research North Wyke. Measurements were carried out Rothamsted Research Harpenden and the Centre of Ecology & Hydrology (Wallingford).\n\nData was collected for the Newton Fund project “UK-China Virtual Joint Centre for Improved Nitrogen Agronomy”. Funded by Biotechnology and Biological Sciences Research Council (BBSRC) and NERC - Ref BB/N013468/1","documentType":"GEMINI_DOCUMENT","familyName":["Reinsch","Lebron","Sánchez-Rodríguez","Carswell","Carter","Chadwick","Cotton","Guyatt","Harvey","Hunt","Jones","Keenan","Lawlor","Marshall","Misselbrook","Patel","Pinder","Pereira","Saunders","Tanna","Thompson","Emmett"],"fullName":["Reinsch, S.","Lebron, I.","Sánchez-Rodríguez, A.R.","Carswell, A.M.","Carter, H.","Chadwick, D.R.","Cotton, J.M.","Guyatt, H.","Harvey, R.","Hunt, A.","Jones, D.L.","Keenan, P.O.","Lawlor, A.J.","Marshall, M.R.","Misselbrook, T.H.","Patel, M.","Pinder, A.P.","Pereira, M.G.","Saunders, K.S.","Tanna, B.","Thompson, N.","Emmett, B.A."],"funder":["Biotechnology and Biological Sciences Research Council"],"givenName":["S.","I.","A.R.","A.M.","H.","D.R.","J.M.","R.","A.","D.L.","P.O.","A.J.","M.R.","T.H.","M.","A.P.","M.G.","K.S.","B.","N.","B.A.","Sabine"],"grant":["BB/N013468/1"],"identifier":"90df9dfa-a0c8-4ead-a13d-0a0a13cda7ab","incomingCitationCount":0,"keyword":["CINAg","CENTRE FOR IMPROVED NITROGEN AGRONOMY","fertilisers","nitrogen","digestate","nitrogen genes","diversity indices","Operational taxonomic unit","microbial community composition","soil properties"],"keywordsOther":["soil properties"],"keywordsProject":["CINAg","CENTRE FOR IMPROVED NITROGEN AGRONOMY"],"keywordsTheme":["fertilisers","nitrogen","digestate","nitrogen genes","diversity indices","Operational taxonomic unit","microbial community composition"],"licence":"Open government licence","lineage":"Soil samples were taken by members of the sites. At North Wyke and Henfaes farm, staff from CEH Bangor helped taking the samples. Two sets of soil cores were taken. One was sent in cooling boxes on ice to CEH Bangor for measuring soil physicochemical properties. The second set was send to Rothamsted Research Harpenden for the analysis of nitrogen genes and microbial community composition (related dataset). The topsoil 0-15 cm were taken with the same type of corer across the sites, samples below 15 cm soil depth were sampled with corers available at the sites. Samples were wet sieved to 5 mm by staff from Bangor University and CEH Bangor. Subsamples of these were analysed for physicochemical and biological parameters.\n\nNitrate (NO3‒), Ammonium (NH4+), Dissolved Organic Carbon (DOC), Dissolved Organic Nitrogen (DON), amino acids, peptides and proteins: extraction with 0.5 M Potassium Sulphate) K2SO4, 5 g soil and 25 ml extractant, after sieving through 5 mm. The supernatant was stored at −20 degrees Celsius until analyses. (Bangor University)\n\nTotal dissolved organic carbon (DOC) and total dissolved Nitrogen (TDN) were measured using a Multi N/C 2100/2100 analyser (AnalytikJena AG, Jena, Germany). Dissolved organic Nitrogen (DON) was calculated by subtracting ammonium (NH4+) and nitrate (NO3−) from the TDN value. (Bangor University)\n\nAmmonium in the supernatant was determined colorimetrically using the salicylate method of Mulvaney (1996) and NO3− following the salicylate method of Miranda et al. (2001) in an Epoch® microplate spectrophotometer (Bio Tek Instruments Inc., Winooski, VT). (Bangor University)\n\nMicrobial biomass carbon and nitrogen were calculated in a similar way than DOC and DON but the 5 grams of soil were kept for 7 days in a desiccator with chloroform (to kill the microorganisms). The difference between DOC, DON after these 7 days (and after extraction as explained before) and the initial values (calculated in the previous paragraph) between 0.45 for microbial C and 0.54 for microbial N were microbial C and microbial N, respectively. (Bangor University)\n\nAmino acids and peptides were measured using a Cary Eclipse Fluorescence Spectrophotometer with a ProStar Solvent Delivery Module (Varian, USA). (Bangor University)\nTexture. Particle size distribution was measured using a Beckman-Coulter LS 13320 laser diffraction particle size analyser.  We manually quarter 0.5 g subsample from the soil, remove organic matter using Hydrogen peroxide (H2O2) and transfer the sample into 250 mL bottle, we added 5 mL of 5 percent Calgon and shaked overnight. The entire contents of the bottle was introduced to the laser analyser for measuring particles size distribution. (CEH Bangor)\n\nSoil water content and loss on ignition (LOI). Soil initially dried at 25 degrees Celsius for 14 days, followed by weight a 10 gram subsample into a crucible and put into an oven at 105 degrees Celsius for 24 hours, the weight loss provides the soil water content. The same crucible with the dry soil is introduced into a Carbolite furnace at 375 degrees Celsius for 16 hours, this second weight loss is quantified and reported as Loss on ignition (LOI). (CEH Bangor)\n\npH and electrical conductivity (EC). In a beaker we add 25ml deionised water into 10g field moist soil; we allowed 30 minutes equilibration time with stirring at time=0 and 15 minutes, we measure pH using Corning 220 pH meter, VWR combination electrode 662-1805. EC is measured after pH in the same beaker using a Jenway 4510 with a 662-1805 electrode. pH in CaCl2 is measured after EC following addition of 2 mL 0.125 mole (M) Calcium Chloride (CaCl2). (CEH Bangor)\n\nPermanganate oxidizable carbon (POXC). We manually quartered 2.5g of soil subsample and analyse for POXC using 0.2M KMnO4 following the method described in Weil et al., 2003. (CEH Bangor)\n\nCitrate-P. We manually quarter 0.5 grams of soil and analyse for citrate extractable phosphorous following the method described in DeLuca et al. (2015). (CEH Bangor)\n\nAggregate size distribution.  We sieved about 1 gram of air-dry soil through a stack of 2 mm and 1 mm sieves, the fraction collected into the 1 mm sieve was introduced into a Beckman-Coulter LS 13320 laser diffraction particle size analyser and measured for the size distribution. (CEH Bangor)\n\nTotal carbon and nitrogen was measured on a Vario EL which uses oxidative combustion followed by thermal conductivity detection (CEH Lancaster).\n\nTotal phosphorus was measured at CEH Lancaster. Total phosphorus is digested with a H2O2/Sulphuric Acid (H2SO4) based digestion reagent mix (along with selenium and lithium sulphate) and subsequent colorimetric analysis carried out on a Seal discrete analyser (CEH Lancaster).\n\nAll results were entered into Excel spreadsheets providing individual datasets for each set of N parameters. Data were exported from Excel as .csv files for ingestion into the EIDC.","locations":["POLYGON((-4.022 50.767, -4.022 53.241, -3.892 53.241, -3.892 50.767, -4.022 50.767))"],"metadataDate":"2026-06-29T16:48:30.000Z","orcid":["https://orcid.org/0000-0003-4649-0677","https://orcid.org/0000-0001-8610-9717","https://orcid.org/0000-0001-8734-2035","https://orcid.org/0000-0003-0580-6698","https://orcid.org/0000-0002-1482-4209","https://orcid.org/0000-0003-2869-0616","https://orcid.org/0000-0002-4594-3606","https://orcid.org/0000-0002-2713-4389"],"organisation":["Centre for Ecology & Hydrology","Bangor University","Rothamsted Research","NERC EDS Environmental Information Data Centre","NERC Environmental Information Data Centre","UK Centre for Ecology & Hydrology"],"publicationDate":"2019-04-26T00:00:00.000Z","recordType":"Dataset","resourceIdentifier":["https://catalogue.ceh.ac.uk/id/90df9dfa-a0c8-4ead-a13d-0a0a13cda7ab","10.5285/90df9dfa-a0c8-4ead-a13d-0a0a13cda7ab","doi:10.5285/90df9dfa-a0c8-4ead-a13d-0a0a13cda7ab"],"resourceType":"Dataset","ror":["https://ror.org/00pggkr55","https://ror.org/006jb1a24","https://ror.org/051h34p92","https://ror.org/04xw4m193"],"shortenedDescription":"The data consist of soil physicochemical and biological data for three soil depths (0-15, 15-30 and 30-60 cm) from a winter wheat field experiment located at two research sites in the United Kingdom. Soil samples were collected between April 2017 and August 2017. Extractions…","state":"published","supplementalDescription":["Miranda, K.M., Espey, M.G., & Wink, D.A. (2001). A Rapid, Simple Spectrophotometric Method for Simultaneous Detection of Nitrate and Nitrite. Nitric Oxide, 5(1), 62–71. https://doi.org/10.1006/niox.2000.0319","Islam, K.R., Stine, M.A., Gruver, J.B., Samson-Liebig, S.E., & Weil, R.R. (2003). Estimating active carbon for soil quality assessment: A simplified method for laboratory and field use. American Journal of Alternative Agriculture, 18(1), 3–17.","DeLuca, T.H., Glanville, H.C., Harris, M., Emmett, B.A., Pingree, M.R.A., de Sosa, L.L., … Jones, D.L. (2015). A novel biologically-based approach to evaluating soil phosphorus availability across complex landscapes. Soil Biology and Biochemistry, 88, 110–119."],"supplementalName":["Centre for Improved Nitrogen Agronomy project website"],"title":"Soil chemical and physical properties from a digestate (winter wheat trial) experiment at North Wyke and Henfaes Farm, UK (2017)","version":1.0,"view":["public","danor"]},{"authorAffiliation":["Bangor University","Rothamsted Research","Centre for Ecology & Hydrology"],"authorFamilyName":["Sánchez-Rodríguez","Carswell","Cotton","Chadwick","Jones","Misselbrook","Saunders","Reinsch"],"authorFullName":["Sánchez-Rodríguez, A.R.","Carswell, A.M.","Cotton, J.M.","Chadwick, D.R.","Jones, D.L.","Misselbrook, T.H.","Saunders, K.S.","Reinsch, S."],"authorGivenName":["A.R.","A.M.","J.M.","D.R.","D.L.","T.H.","K.S.","S."],"authorOrcid":["https://orcid.org/0000-0001-8734-2035","https://orcid.org/0000-0003-0580-6698","https://orcid.org/0000-0002-1482-4209","https://orcid.org/0000-0002-4594-3606","https://orcid.org/0000-0003-4649-0677"],"authorRor":["https://ror.org/006jb1a24","https://ror.org/0347fy350","https://ror.org/00pggkr55"],"availability":"Available","catalogue":"eidc","dataFormat":["Comma-separated values (CSV)"],"description":"The data contains nitrogen (N) offtake, N emissions (ammonia and nitrous oxide), soil parameters (pH, EC, NH4+, NO3−), biomass and grain production from a winter wheat field experiment located at Bangor University (Henfaes Research Station in North Wales) and Rothamsted Research (North Wyke (NW) in Devon). Data were collected between  April 2017 and August 2017. Measurements and soil and plant samples were taken from 45 plots in a randomized complete block design. Sixteen extra mini-plots at the prevailing wind (south westerly) edge of the experiment site were used at NW for NH3 volatilization measurements. The treatments were control (C, without N application), food based digestate (D), food based digestate + the nitrification inhibitor 3,4-dimethylpyrazole (DMPP; D+NI), acidified food based digestate (AD), acidified food based digestate + the nitrification inhibitor 3,4-dimethylpyrazole (DMPP; AD+NI). Four rates of ammonium nitrate (NH4NO3; 75, 150, 225 and 300 kg N ha−1)  were applied to compare yields and fertilizer replacement rate of the digestate treatments (targeted at 190 kg N  ha−1). Soil pH, soil electrical conductivity (EC), soil NH4+ and soil NO3−, NH3 volatilization, N2O emissions were measured periodically for C, D, D+NI, AD and AD+NI and nitrogen concentration in grain and straw, grain yield and plant biomass for all treatments.\n\nNitrogen offtake, losses and fluxes were used to determine the N use efficiency (NUE) and fertilizer replacement rate of the digestate treatments.\n\nMeasurements were undertaken by members of staff from Bangor University, School of Environment, Natural Resources & Geography and Rothamsted Research, Sustainable Agricultural Sciences – North Wyke.\n\nData was collected for the Newton Fund project “UK-China Virtual Joint Centre for Improved Nitrogen Agronomy”. Funded by Biotechnology and Biological Sciences Research Council (BBSRC) and NERC -  Ref BB/N013468/1","documentType":"GEMINI_DOCUMENT","familyName":["Reinsch","Sánchez-Rodríguez","Carswell","Cotton","Chadwick","Jones","Misselbrook","Saunders"],"fullName":["Reinsch, S.","Sánchez-Rodríguez, A.R.","Carswell, A.M.","Cotton, J.M.","Chadwick, D.R.","Jones, D.L.","Misselbrook, T.H.","Saunders, K.S."],"funder":["Biotechnology and Biological Sciences Research Council"],"givenName":["S.","A.R.","A.M.","J.M.","D.R.","D.L.","T.H.","K.S."],"grant":["BB/N013468/1"],"identifier":"836dfb2e-bc6f-47d2-b924-8261be671391","incomingCitationCount":0,"keyword":["CINAg","CENTRE FOR IMPROVED NITROGEN AGRONOMY","Land use","Soil","Agriculture","nitrification inhibitor","digestate","Nitrogen use efficiency (NUE)","nitrogen","fertiliser","nitrification","winter wheat"],"keywordsOther":["nitrification inhibitor","digestate","Nitrogen use efficiency (NUE)","nitrogen","fertiliser","nitrification","winter wheat"],"keywordsProject":["CINAg","CENTRE FOR IMPROVED NITROGEN AGRONOMY"],"keywordsTheme":["Land use","Soil","Agriculture"],"licence":"Open government licence","lineage":"Cumulative ammonia (NH3) emissions were measured using a wind tunnel technique (with 0.02 M H3PO4 acid traps) at a daily resolution for seven days following digestate application, except the first day where NH3 volatilization was measured twice at Henfaes Farm and three times at North Wyke.\n\nNitrous oxide emissions were measured using a combination of static manual and static automatic (combined with an Isotopic  Nitrous Oxide (N2O) Analyser, Los Gatos Research Inc. San Jose, CA, USA) chambers at Henfaes Farm, and only static manual chambers at North Wyke. Using the manual static chambers, the resolution of Nitrous Oxide (N2O) measurements following Nitrogen (N) fertiliser application was 3 x weekly for weeks 1 and 2, 2 x weekly for weeks 3 and 4, and 1 x weekly thereafter. Using the automatic static chambers, the resolution of N2O measurements following N fertiliser application was approximately every six hours.\n\nSamples for soil ammonium (NH4+) and nitrate (NO3−) analyses were collected at the same temporal resolution as the N2O sampling using manual static chambers.\n\nWinter wheat yield and biomass measurements were done the day of harvest (fresh weight) and subsamples were used to determine grass and grain moisture after 24 hours at 80 degrees Celsius.\n\nAll results were entered into Excel spreadsheets providing individual datasets for each set of N parameters. Data were exported from Excel as .csv files for ingestion into the EIDC.","locations":["POLYGON((-4.022 50.767, -4.022 53.241, -3.892 53.241, -3.892 50.767, -4.022 50.767))"],"metadataDate":"2026-06-29T16:16:29.000Z","orcid":["https://orcid.org/0000-0003-4649-0677","https://orcid.org/0000-0001-8734-2035","https://orcid.org/0000-0003-0580-6698","https://orcid.org/0000-0002-1482-4209","https://orcid.org/0000-0002-4594-3606"],"organisation":["UK Centre for Ecology & Hydrology","Bangor University","Rothamsted Research","Centre for Ecology & Hydrology","NERC EDS Environmental Information Data Centre","NERC Environmental Information Data Centre"],"publicationDate":"2019-04-25T00:00:00.000Z","recordType":"Dataset","resourceIdentifier":["https://catalogue.ceh.ac.uk/id/836dfb2e-bc6f-47d2-b924-8261be671391","10.5285/836dfb2e-bc6f-47d2-b924-8261be671391","doi:10.5285/836dfb2e-bc6f-47d2-b924-8261be671391"],"resourceType":"Dataset","ror":["https://ror.org/00pggkr55","https://ror.org/006jb1a24","https://ror.org/0347fy350","https://ror.org/04xw4m193"],"shortenedDescription":"The data contains nitrogen (N) offtake, N emissions (ammonia and nitrous oxide), soil parameters (pH, EC, NH4+, NO3−), biomass and grain production from a winter wheat field experiment located at Bangor University (Henfaes Research Station in North Wales) and Rothamsted…","state":"published","supplementalDescription":["Project website"],"supplementalName":["THE UK-CHINA VIRTUAL JOINT CENTRE FOR IMPROVED NITROGEN AGRONOMY (CINAG)"],"title":"Greenhouse gas emissions, nitrogen use efficiency and biomass from digestate experiments on winter wheat at North Wyke and Henfaes, UK (2017)","topic":["0/Land use/","0/Soil/","0/Agriculture/"],"version":1.0,"view":["public","danor"]},{"authorAffiliation":["Centre for Ecology & Hydrology","Unaffiliated","Bangor University","Rothamsted Research"],"authorFamilyName":["Reinsch","Carswell","Cowan","Lebron","Sánchez-Rodríguez","Barrett","Carter","Chadwick","Cotton","Guyatt","Harvey","Hunt","Jones","Keenan","Lawlor","Marshall","Misselbrook","Patel","Pereira","Saunders","Shaw","Skiba","Tanna","Thompson","Emmett"],"authorFullName":["Reinsch, S.","Carswell, A.M.","Cowan, N.J.","Lebron, I.","Sánchez-Rodríguez, A.R.","Barrett, G.","Carter, H.T.","Chadwick, D.R.","Cotton, J.M.","Guyatt, H.J.","Harvey, R.","Hunt, A.","Jones, D.L.","Keenan, P.O.","Lawlor, A.J.","Marshall, M.R.","Misselbrook, T.H.","Patel, M.","Pereira, M.G.","Saunders, K.S.","Shaw, R.","Skiba, U.M.","Tanna, B.","Thompson, N.","Emmett, B.A."],"authorGivenName":["S.","A.M.","N.J.","I.","A.R.","G.","H.T.","D.R.","J.M.","H.J.","R.","A.","D.L.","P.O.","A.J.","M.R.","T.H.","M.","M.G.","K.S.","U.M.","B.","N.","B.A."],"authorOrcid":["https://orcid.org/0000-0003-4649-0677","https://orcid.org/0000-0003-0580-6698","https://orcid.org/0000-0001-8610-9717","https://orcid.org/0000-0001-8734-2035","https://orcid.org/0000-0002-1482-4209","https://orcid.org/0000-0003-2869-0616","https://orcid.org/0000-0002-4594-3606","https://orcid.org/0000-0001-8659-6092","https://orcid.org/0000-0002-2713-4389"],"authorRor":["https://ror.org/00pggkr55","https://ror.org/006jb1a24","https://ror.org/0347fy350"],"availability":"Available","catalogue":"eidc","dataFormat":["Comma-separated values (CSV)"],"description":"The data consist of soil physicochemical and biological data for three soil depths (0-15, 15-30 and 30-60 cm) from a three-cut silage plot trial located at three grassland sites within the UK collected between April 2016 and October 2016. The sites were Rothamsted Research at North Wyke in Devon, Bangor University at Henfaes Research Station in North Wales, and Easter Bush in Scotland.\n\nAt each site measurements were taken from sixteen plots, organised within a randomised complete block design: four (control) plots did not receive fertilizer, four plots received urea only,  four plots received urea and urea-inhibitors, and four plots received ammonium-nitrate (Nitram). Fertiliser was applied three times and three cuts were performed. All parameters were measured following fertiliser application. Samples were taken before fertilizer additions at peak growth and before the last silage cut.\n\nSoil physical parameters were: aggregate size distribution, aggregate stability, texture (sand/silt/clay) and soil moisture. Soil chemical parameters were: soil nitrate and ammonium, dissolved organic carbon and nitrogen, amino acids and peptides, soil organic matter content as loss-on-ignition, pH, sodium, potassium, calcium, magnesium, permanganate oxdisable carbon, citric acid extractable phosphorous, Olsen-P and total carbon, nitrogen and phosphorus. Soil biological measures were: microbial biomass, carbon and nitrogen. \n\nMicrobial community composition and nitrogen genes were measured on the same soil samples and are presented in a separate dataset (https://doi.org/10.5285/59f81d41-a789-4c5c-8ab8-36baa7ac2c55)\n\nMeasurements were undertaken by members of staff from the Centre of Ecology & Hydrology (Bangor, Edinburgh, Lancaster, Wallingford), Bangor University, School of Environment, Natural Resources & Geography and Rothamsted Research, Sustainable Agricultural Sciences, North Wyke.\n\nData was collected for the Newton Fund project \"UK-China Virtual Joint Centre for Improved Nitrogen Agronomy\". Funded by Biotechnology and Biological Sciences Research Council (BBSRC) and NERC -  Ref BB/N013468/1","documentType":"GEMINI_DOCUMENT","familyName":["Reinsch","Carswell","Cowan","Lebron","Sánchez-Rodríguez","Barrett","Carter","Chadwick","Cotton","Guyatt","Harvey","Hunt","Jones","Keenan","Lawlor","Marshall","Misselbrook","Patel","Pereira","Saunders","Shaw","Skiba","Tanna","Thompson","Emmett"],"fullName":["Reinsch, S.","Carswell, A.M.","Cowan, N.J.","Lebron, I.","Sánchez-Rodríguez, A.R.","Barrett, G.","Carter, H.T.","Chadwick, D.R.","Cotton, J.M.","Guyatt, H.J.","Harvey, R.","Hunt, A.","Jones, D.L.","Keenan, P.O.","Lawlor, A.J.","Marshall, M.R.","Misselbrook, T.H.","Patel, M.","Pereira, M.G.","Saunders, K.S.","Shaw, R.","Skiba, U.M.","Tanna, B.","Thompson, N.","Emmett, B.A."],"funder":["Biotechnology and Biological Sciences Research Council"],"givenName":["S.","A.M.","N.J.","I.","A.R.","G.","H.T.","D.R.","J.M.","H.J.","R.","A.","D.L.","P.O.","A.J.","M.R.","T.H.","M.","M.G.","K.S.","U.M.","B.","N.","B.A."],"grant":["BB/N013468/1"],"identifier":"7a87dde4-b54e-49b0-8751-1d59e8aebb90","incomingCitationCount":0,"keyword":["CENTRE FOR IMPROVED NITROGEN AGRONOMY (CINAG)","Soil","Grassland","grassland","fertiliser","nitrogen","Urease inhibitor","soil"],"keywordsOther":["Grassland","grassland","fertiliser","nitrogen","Urease inhibitor","soil"],"keywordsProject":["CENTRE FOR IMPROVED NITROGEN AGRONOMY (CINAG)"],"keywordsTheme":["Soil"],"licence":"Open government licence","lineage":"Soil samples were taken by members of the North Wyke, Easter Bush and Henfaes farm sites. At North Wyke and Henfaes farm, staff from CEH Bangor helped taking the samples. Two sets of soil cores were taken. One core was sent in cooling boxes on ice to CEH Bangor for measuring soil physicochemical properties. The second set was send to Rothamsted Research Harpenden for the analysis of nitrogen genes and microbial community composition (related dataset). The topsoil 0-15 cm were taken with the same type of corer across the sites, samples below 15 cm soil depth were sampled with corers available at the sites. Samples were wet sieved to 5 mm by staff from Bangor University and CEH Bangor. Subsamples of these were analysed for physicochemical and biological parameters.\n\nNitrate (NO3‒), Ammonium (NH4+) ,Dissolved Organic Carbon (DOC), Dissolved Organic Nitrogen (DON), amino acids, peptides and proteins: extraction with 0.5 M potassium sulphate (K2SO4), 5 g soil and 25 ml extractant, after sieving through 5 mm. The supernatant was stored at −20 degrees Celsius until analyses. (Bangor University)\n\nTotal dissolved organic carbon (DOC) and total dissolved N (TDN) were measured using a Multi N/C 2100/2100 analyser (AnalytikJena AG, Jena, Germany). Dissolved organic N (DON) was calculated by subtracting NH4+ and NO3− from the TDN value. (Bangor University)\n\nAmmonium in the supernatant was determined colorimetrically using the salicylate method of Mulvaney (1996) and NO3− following the salicylate method of Miranda et al. (2001) in an Epoch® microplate spectrophotometer (Bio Tek Instruments Inc., Winooski, VT). (Bangor University)\n\nMicrobial biomass carbon and nitrogen were calculated in a similar way than DOC and DON but the 5 grams of soil were kept for 7 days in a desiccator with chloroform (to kill the microorganisms). The difference between DOC, DON after these 7 days (and after extraction as explained before) and the initial values (calculated in the previous paragraph) between 0.45 for microbial C and 0.54 for microbial N were microbial C and microbial N, respectively. (Bangor University)\n\nAmino acids and peptides were measured using a Cary Eclipse Fluorescence Spectrophotometer with a ProStar Solvent Delivery Module (Varian, USA). (Bangor University)\nTexture. Particle size distribution was measured using a Beckman-Coulter LS 13320 laser diffraction particle size analyser.  We manually quarter 0.5 g subsample from the soil, remove organic matter using Hydrogen peroxide (H2O2) and transfer the sample into 250 mL bottle, we added 5 mL of 5 percent Calgon and shaked overnight. The entire contents of the bottle was introduced to the laser analyser for measuring particles size distribution. (CEH Bangor)\n\nSoil water content and loss on ignition (LOI). Soil initially dried at 25 degrees Celsius for 14 days, followed by weight a 10 gram subsample into a crucible and put into an oven at 105 degrees Celsius for 24 hours, the weight loss provides the soil water content. The same crucible with the dry soil is introduced into a Carbolite furnace at 375 degrees Celsius for 16 hours, this second weight loss is quantified and reported as loss on ignition (LOI). (CEH Bangor)\n\npH and electrical conductivity (EC). In a beaker we added 25ml deionised water into 10g field moist soil; we allowed 30 minutes equilibration time with stirring at time=0 and 15 minutes, we measured pH using Corning 220 pH meter, VWR combination electrode 662-1805. EC is measured after pH in the same beaker using a Jenway 4510 with a 662-1805 electrode. pH in CaCl2 is measured after EC following addition of 2 mL 0.125 moles (M) Calcium Chloride (CaCl2). (CEH Bangor)\n\nPermanganate oxidizable carbon (POXC). We manually quarter 2.5g of soil subsample and analyse for POXC using 0.2M KMnO4 following the method described in Weil et al., 2003. (CEH Bangor)\n\nCitrate-P. We manually quarter 0.5 grams of soil and analyse for citrate extractable phosphorous following the method described in DeLuca et al. (2015). (CEH Bangor)\n\nAggregate size distribution.  We sieved about 1 gram of air-dry soil through a stack of 2 mm and 1 mm sieves, the fraction collected into the 1 mm sieve was introduced into a Beckman-Coulter LS 13320 laser diffraction particle size analyser and measured for the size distribution. (CEH Bangor)\n\nTotal carbon and nitrogen was measured on a Vario EL which uses oxidative combustion followed by thermal conductivity detection (CEH Lancaster).\n\nTotal phosphorus was measured at CEH Lancaster. Total phosphorus is digested with a H2O2/Sulphuric Acid (H2SO4) based digestion reagent mix (along with selenium and lithium sulphate) and subsequent colorimetric analysis carried out on a Seal discrete analyser (CEH Lancaster).\n\nAll results were entered into Excel spreadsheets providing individual datasets for each set of N parameters. Data were exported from Excel as .csv files for ingestion into the EIDC. ","locations":["POLYGON((-4.022 50.767, -4.022 55.873, -3.191 55.873, -3.191 50.767, -4.022 50.767))"],"metadataDate":"2026-06-29T17:10:05.000Z","orcid":["https://orcid.org/0000-0003-4649-0677","https://orcid.org/0000-0003-0580-6698","https://orcid.org/0000-0001-8610-9717","https://orcid.org/0000-0001-8734-2035","https://orcid.org/0000-0002-1482-4209","https://orcid.org/0000-0003-2869-0616","https://orcid.org/0000-0002-4594-3606","https://orcid.org/0000-0001-8659-6092","https://orcid.org/0000-0002-2713-4389"],"organisation":["UK Centre for Ecology & Hydrology","Centre for Ecology & Hydrology","Unaffiliated","Bangor University","Rothamsted Research","NERC EDS Environmental Information Data Centre","NERC Environmental Information Data Centre"],"publicationDate":"2019-04-26T00:00:00.000Z","recordType":"Dataset","resourceIdentifier":["https://catalogue.ceh.ac.uk/id/7a87dde4-b54e-49b0-8751-1d59e8aebb90","10.5285/7a87dde4-b54e-49b0-8751-1d59e8aebb90","doi:10.5285/7a87dde4-b54e-49b0-8751-1d59e8aebb90"],"resourceType":"Dataset","ror":["https://ror.org/00pggkr55","https://ror.org/006jb1a24","https://ror.org/0347fy350","https://ror.org/04xw4m193"],"shortenedDescription":"The data consist of soil physicochemical and biological data for three soil depths (0-15, 15-30 and 30-60 cm) from a three-cut silage plot trial located at three grassland sites within the UK collected between April 2016 and October 2016. The sites were Rothamsted Research…","state":"published","supplementalDescription":["Project website","Related paper"],"supplementalName":["CENTRE FOR IMPROVED NITROGEN AGRONOMY"," Miranda et al. (2001)","Weil et al., (2003)","DeLuca et al. (2015)"],"title":"Soil chemical and physical properties from inorganic fertiliser additions to grassland at North Wyke, Henfaes and Easter Bush, UK (2016)","topic":["0/Soil/"],"version":1.0,"view":["public","danor"]},{"authorAffiliation":["UK Centre for Ecology & Hydrology"],"authorFamilyName":["Cowan"],"authorFullName":["Cowan, N."],"authorGivenName":["N."],"authorRor":["https://ror.org/00pggkr55"],"availability":"Available","catalogue":"eidc","dataFormat":["Comma-separated values (CSV)"],"description":"Data from 38 experimental sites across the UK and Ireland were collated resulting in 623 separate mineral fertiliser N2O emission factors (EF) estimates derived from field measurements. Data were either i) extracted from published studies in which one aim of the experimentation was to explicitly measure N2O and report EFs after a mineral fertiliser application, or ii) raw data were used from the Agricultural and Environmental Data Archive (AEDA).\n\nTo find the published data, a survey of literature was conducted using Google Scholar for articles considered ‘recent’ (20 years or fewer), i.e. published after January 1998 and submitted before April 2019. The following search terms and their variations were used: N2O, nitrous oxide, emission factor, mineral fertiliser, ammonium nitrate, urea, nitrification inhibitor, nitrogen use efficiency, agriculture, greenhouse gas, grassland and arable. This search based on keywords was complemented with a search through the literature cited in the articles found and known previous research.","documentType":"GEMINI_DOCUMENT","familyName":["Cowan"],"fullName":["Cowan, N."],"funder":["Biotechnology and Biological Sciences Research Council","Natural Environment Research Council"],"givenName":["N."],"grant":["BB/N013468/1","NE/S003614/1"],"identifier":"9948d1b9-caa1-4894-93e6-cc0f4326fced","incomingCitationCount":1,"keyword":["Agriculture","ammonium nitrate","nitrous oxide","urea","N2O","nitrification inhibitor","nitrogen use efficiency","greenhouse gas","arable farming","grassland","agriculture","mineral fertiliser","nitrogen oxides","nitrification","emission factor"],"keywordsOther":["ammonium nitrate","nitrous oxide","urea","N2O","nitrification inhibitor","nitrogen use efficiency","greenhouse gas","arable farming","grassland","agriculture","mineral fertiliser","nitrogen oxides","nitrification","emission factor"],"keywordsTheme":["Agriculture"],"licence":"Open government licence","lineage":"Raw data extracted from the Agricultural and Environmental Data Archive (AEDA) was used to calculate emission factors  (EFs) for 25 days after fertiliser application using the log-normal Bayesian approach as described in Levy et al., 2017, Cowan et al., 2019a, which accounts for the log-normal spatial and temporal distribution of N2O fluxes after fertiliser events. Fertilisers treated with nitrification and urease inhibitors in these studies were included for comparison.","locations":["POLYGON((-10.757 49.864, -10.757 60.861, 1.768 60.861, 1.768 49.864, -10.757 49.864))"],"metadataDate":"2026-06-27T19:22:33.000Z","organisation":["UK Centre for Ecology & Hydrology","NERC Environmental Information Data Centre","NERC EDS Environmental Information Data Centre"],"publicationDate":"2021-04-08T00:00:00.000Z","recordType":"Dataset","resourceIdentifier":["https://catalogue.ceh.ac.uk/id/9948d1b9-caa1-4894-93e6-cc0f4326fced","10.5285/9948d1b9-caa1-4894-93e6-cc0f4326fced","doi:10.5285/9948d1b9-caa1-4894-93e6-cc0f4326fced"],"resourceType":"Dataset","rightsHolder":["UK Centre for Ecology & Hydrology"],"ror":["https://ror.org/00pggkr55","https://ror.org/04xw4m193"],"shortenedDescription":"Data from 38 experimental sites across the UK and Ireland were collated resulting in 623 separate mineral fertiliser N2O emission factors (EF) estimates derived from field measurements. Data were either i) extracted from published studies in which one aim of the experimentation…","state":"published","supplementalDescription":["\n"],"supplementalName":["Levy, P.E., Cowan, N., van Oijen, M., Famulari, D., Drewer, J., Skiba, U., 2017. Estimation of cumulative fluxes of nitrous oxide: uncertainty in temporal upscaling and emission factors. European Journal of Soil Science 68, 400–411."],"title":" Nitrous oxide emission factors of mineral fertilisers in the UK and Ireland (1999-2019)","topic":["0/Agriculture/"],"version":1.0,"view":["public","crimar"]},{"authorAffiliation":["University of Birmingham"],"authorFamilyName":["Cambronero","Williams","Orsini","Colbourne"],"authorFullName":["Cambronero, M.C.","Williams, T.D.","Orsini, L.","Colbourne, J.K."],"authorGivenName":["M.C.","T.D.","L.","J.K."],"authorOrcid":["https://orcid.org/0000-0002-5342-2165","https://orcid.org/0000-0002-5857-3851","https://orcid.org/0000-0002-1716-5624","https://orcid.org/0000-0002-6966-2972"],"availability":"Available","catalogue":"eidc","dataFormat":["Comma-separated values (CSV)"],"description":"This dataset contains fitness-linked life history traits, environmental data, and protein and gene expression data for Daphnia magna.\n \nLife history data were measured in common garden experiments. For each clone of the same genotype across all experiments, size at maturity (distance between the head and the base of the tail spine), age at maturity (first time eggs were observed in the brood chamber), fecundity (total number of offspring released summing first and second brood), and mortality were measured. Critical thermal maximum (CTmax) was measured on the experimental animals.\n \nEnvironmental data consisted of Secchi disk depth (water transparency) ; total phosphorous; and total nitrogen for the years 1971–1999; a record of pesticides 1955–2010; temperature records over the past century 80 km from Lake Ring.\n \nOrganic and carbonate contents of the sediment was estimated using the loss on ignition (LOI) method.\n \nHb protein data consisted of constitutive Hb protein crude content animals reared in normoxic (saturated oxygen level) conditions at two experimental temperatures, 20 and 30°C.\n \nHeat shock protein expression was measured in four heat shock proteins (HSP20, HSP60, HSP70 and HSP90). Total RNA, qPCR, mean CT (cycle threshold) value per sample and per protein were collected.","documentType":"GEMINI_DOCUMENT","familyName":["Cambronero","Williams","Orsini","Colbourne"],"fullName":["Cambronero, M.C.","Williams, T.D.","Orsini, L.","Colbourne, J.K."],"funder":["Natural Environment Research Council"],"givenName":["M.C.","T.D.","L.","J.K."],"grant":["NE/N016777/1"],"identifier":"13f878dc-ef87-45d5-be8c-5733c88c30e9","incomingCitationCount":0,"keyword":["Resurrection ecology","Freshwater ecology","Experimental evolution","Daphnia magna","Fitness traits","pesticide","eutrophication"],"keywordsOther":["Resurrection ecology","Freshwater ecology","Experimental evolution","Daphnia magna","Fitness traits","pesticide","eutrophication"],"licence":"Open government licence","lineage":"Lineages (genotypes) of Daphnia were resurrected (revived) from biological archives of Lake Ring, a shallow mixed lake in Denmark (550 57´ 51.83´´ N, 9035´46.87´´E) with a known history of anthropogenic impact. The process by which lineages were obtained is as follows:\n1.\tSediment is sampled using a piston corer of approximately 1.5 m in length with an internal tube diameter of 14 cm\n2.\tThe sediment core is separated in incremental layers of 1 cm or less using a flat metal surface\n3.\tEach sediment layer is collected in a separate sampling bag and weighted using a precision scale\n4.\tEach layer of sediment is sieved and dormant Daphnia isolated after visual inspection\n5.\tUnder a stereo microscope, each dormant Daphnia encapsulated in an ephippium is opened using microdissection forceps to expose dormant embryos to light\n6.\tEach dormant embryos develops in a fully functional adult from which an isoclonal lineage is established and maintained in the laboratory indefinitely.\nFrom a total of 923 dormant Daphnia, 262 lineages were successfully hatched.  From these, a total of 40 randomly chosen lineages across the sediment core were used in the experiments and fitness-linked life-history traits measured. ","locations":["POLYGON((9.588 55.961, 9.588 55.968, 9.605 55.968, 9.605 55.961, 9.588 55.961))"],"metadataDate":"2026-06-27T18:49:00.000Z","orcid":["https://orcid.org/0000-0002-5342-2165","https://orcid.org/0000-0002-5857-3851","https://orcid.org/0000-0002-1716-5624","https://orcid.org/0000-0002-6966-2972"],"organisation":["University of Birmingham","NERC EDS Environmental Information Data Centre"],"publicationDate":"2022-02-03T00:00:00.000Z","recordType":"Dataset","resourceIdentifier":["https://catalogue.ceh.ac.uk/id/13f878dc-ef87-45d5-be8c-5733c88c30e9","10.5285/13f878dc-ef87-45d5-be8c-5733c88c30e9","doi:10.5285/13f878dc-ef87-45d5-be8c-5733c88c30e9"],"resourceType":"Dataset","rightsHolder":["University of Birmingham"],"ror":["https://ror.org/04xw4m193"],"shortenedDescription":"This dataset contains fitness-linked life history traits, environmental data, and protein and gene expression data for Daphnia magna.\n \nLife history data were measured in common garden experiments. For each clone of the same genotype across all experiments, size at…","state":"published","supplementalName":["Data from: Predictability of the impact of multiple stressors on the keystone species Daphnia","Data from: Evolution of thermal tolerance in multifarious environments","Evolutionary mechanisms underpinning fitness response to multiple stressors in Daphnia"],"title":"Phenotypic and environmental data for Lake Ring, Denmark, Daphnia magna population, 1955-2010","version":1.0,"view":["public","matnic"]},{"authorAffiliation":["Rothamsted Research","Bangor University","Unaffiliated","Centre for Ecology & Hydrology"],"authorFamilyName":["Carswell","Shaw","Sánchez-Rodríguez","Cotton","Chadwick","Jones","Misselbrook","Saunders","Hunt","Reinsch"],"authorFullName":["Carswell, A.M.","Shaw, R.","Sánchez-Rodríguez, A.R.","Cotton, J.M.","Chadwick, D.R.","Jones, D.L.","Misselbrook, T.H.","Saunders, K.S.","Hunt, J.","Reinsch, S."],"authorGivenName":["A.M.","R.","A.R.","J.M.","D.R.","D.L.","T.H.","K.S.","J.","S."],"authorOrcid":["https://orcid.org/0000-0003-0580-6698","https://orcid.org/0000-0001-8734-2035","https://orcid.org/0000-0002-1482-4209","https://orcid.org/0000-0002-4594-3606","https://orcid.org/0000-0003-4649-0677"],"authorRor":["https://ror.org/051h34p92","https://ror.org/006jb1a24","https://ror.org/00pggkr55"],"availability":"Available","catalogue":"eidc","dataFormat":["Comma-separated values (CSV)"],"description":"The data consists of nitrogen (N) offtake, N emissions and soil N parameters, and herbage quality parameters from a three-cut silage plot trial located at two grassland sites within the UK collected between April and October 2016. The sites were Rothamsted Research at North Wyke in Devon and Bangor University at Henfaes Research Station in North Wales. At each site measurements were taken from 16 plots, organised within a randomised complete block design. Fertiliser was applied three times and three cuts were performed, all parameters measured were following a fertiliser application. Nitrogen parameters measured were crude protein (CP) of herbage, ammonia (NH3) emissions, nitrous oxide (N2O) emissions, and soil ammonium (NH4) and nitrate (NO3). Herbage quality parameters measured were dry matter, acid-digestible fibre (ADF), ash, CP, metabolizable energy (ME), and non-digestible fibre (NDF) and digestibility (D) was calculated. \n\nNitrogen offtake, losses and fluxes were measured to determine the N use efficiency and the economic viability of different N fertilisers.\nMeasurements were undertaken by members of staff from Bangor University, School of Environment, Natural Resources & Geography and Rothamsted Research, Sustainable Agricultural Sciences – North Wyke.\n\nData was collected for the Newton Fund project \"UK-China Virtual Joint Centre for Improved Nitrogen Agronomy\". Funded by Biotechnology and Biological Sciences Research Council (BBSRC) and NERC -  Ref BB/N013468/1","documentType":"GEMINI_DOCUMENT","familyName":["Reinsch","Carswell","Shaw","Sánchez-Rodríguez","Cotton","Chadwick","Jones","Misselbrook","Saunders","Hunt"],"fullName":["Reinsch, S.","Carswell, A.M.","Shaw, R.","Sánchez-Rodríguez, A.R.","Cotton, J.M.","Chadwick, D.R.","Jones, D.L.","Misselbrook, T.H.","Saunders, K.S.","Hunt, J."],"funder":["Biotechnology and Biological Sciences Research Council"],"givenName":["Sabine","A.M.","R.","A.R.","J.M.","D.R.","D.L.","T.H.","K.S.","J.","S."],"grant":["BB/N013468/1"],"identifier":"4c7d4b3c-88f7-43ab-a50f-b6804474e568","incomingCitationCount":0,"keyword":["CINAg","Centre for Improved Nitrogen Agronomy","Agriculture","Climate and climate change","Pollution","Urease inhibitor","Nitrogen use efficiency (NUE)","GHG","fertiliser","nitrogen","grassland","greenhouse gas"],"keywordsOther":["Urease inhibitor","Nitrogen use efficiency (NUE)","GHG","fertiliser","nitrogen","grassland","greenhouse gas"],"keywordsProject":["CINAg","Centre for Improved Nitrogen Agronomy"],"keywordsTheme":["Agriculture","Climate and climate change","Pollution"],"licence":"Open government licence","lineage":"At each site measurements were taken from sixteen plots, organised within a randomised complete block design. Four plots did not receive fertilizers (controls), four plots received urea only, four plots received urea and urea-inhibitors, and 4 plots received Ammonium-nitrate (Nitram).\n\nCumulative NH3 emissions were measured using a wind tunnel technique, at a daily resolution for 21 days following each N fertiliser application. \nNitrous oxide emissions were measured using a combination of static manual and static automatic (combined with an Isotopic N2O Analyser, Los Gatos Research Inc. San Jose, CA, USA) chambers. Using the manual static chambers, the resolution of N2O measurements following N fertiliser application was 3 x weekly for weeks 1 and 2, 2 x weekly for weeks 3 and 4, and 1 x weekly thereafter. Using the automatic static chambers, the resolution of N2O measurements following N fertiliser application was approximately every six hours.\n\nSamples for soil NH4 and NO3 analyses were collected at the same temporal resolution as the N2O sampling using manual static chambers.\nHerbage yield measurements were carried out on the day of cutting, and samples of the fresh-cut herbage were couriered within 24 h to external contractors (Trouw Nutrition GB, Blenheim House, Ashbourne, UK and Sciantec Analytical Laboratories, Stockbridge Technology Centre, York, UK) for quality analyses of (CP, ME, NDF, ADF and DM). Digestibility was calculated.\n\nAll results were entered into Excel spreadsheets providing individual datasets for each set of N parameters. Data were exported from Excel as .csv files for ingestion into the EIDC.","locations":["POLYGON((-4.022 50.767, -4.022 53.241, -3.892 53.241, -3.892 50.767, -4.022 50.767))"],"metadataDate":"2026-06-29T16:27:16.000Z","orcid":["https://orcid.org/0000-0003-0580-6698","https://orcid.org/0000-0001-8734-2035","https://orcid.org/0000-0002-1482-4209","https://orcid.org/0000-0002-4594-3606","https://orcid.org/0000-0003-4649-0677"],"organisation":["UK Centre for Ecology & Hydrology","Rothamsted Research","Bangor University","Unaffiliated","Centre for Ecology & Hydrology","NERC EDS Environmental Information Data Centre","NERC Environmental Information Data Centre"],"publicationDate":"2019-04-16T00:00:00.000Z","recordType":"Dataset","resourceIdentifier":["https://catalogue.ceh.ac.uk/id/4c7d4b3c-88f7-43ab-a50f-b6804474e568","10.5285/4c7d4b3c-88f7-43ab-a50f-b6804474e568","doi:10.5285/4c7d4b3c-88f7-43ab-a50f-b6804474e568"],"resourceType":"Dataset","ror":["https://ror.org/00pggkr55","https://ror.org/051h34p92","https://ror.org/006jb1a24","https://ror.org/04xw4m193"],"shortenedDescription":"The data consists of nitrogen (N) offtake, N emissions and soil N parameters, and herbage quality parameters from a three-cut silage plot trial located at two grassland sites within the UK collected between April and October 2016. The sites were Rothamsted Research…","state":"published","supplementalDescription":["Project web site"],"supplementalName":["THE UK-CHINA VIRTUAL JOINT CENTRE FOR IMPROVED NITROGEN AGRONOMY (CINAG)"],"title":"Greenhouse gas emissions, nitrogen use efficiency and biomass from inorganic fertiliser additions to grassland at North Wyke and Henfaes, UK (2016)","topic":["0/Agriculture/","0/Climate and climate change/","0/Pollution/"],"version":1.0,"view":["public","danor"]},{"authorAffiliation":["Rothamsted Research","Centre for Ecology & Hydrology","Bangor University"],"authorFamilyName":["Blaud","Abadie","Clark","Hirsch","Griffiths","Goodall","Carswell","Misselbrook","Shaw","Sánchez-Rodríguez","Chadwick","Jones","Cowan","Skiba","Reinsch"],"authorFullName":["Blaud, A.","Abadie, M.","Clark, I.M.","Hirsch, P.R.","Griffiths, R.I.","Goodall, T.I.","Carswell, A.M.","Misselbrook, T.H.","Shaw, R.","Sánchez-Rodríguez, A.R.","Chadwick, D.R.","Jones, D.L.","Cowan, N.J.","Skiba, U.","Reinsch, S."],"authorGivenName":["A.","M.","I.M.","P.R.","R.I.","T.I.","A.M.","T.H.","R.","A.R.","D.R.","D.L.","N.J.","U.","S."],"authorOrcid":["https://orcid.org/0000-0003-0860-8080","https://orcid.org/0000-0002-1589-5420","https://orcid.org/0000-0002-3341-4547","https://orcid.org/0000-0003-0580-6698","https://orcid.org/0000-0002-4594-3606","https://orcid.org/0000-0001-8734-2035","https://orcid.org/0000-0002-1482-4209","https://orcid.org/0000-0001-8659-6092","https://orcid.org/0000-0003-4649-0677"],"authorRor":["https://ror.org/0347fy350","https://ror.org/00pggkr55","https://ror.org/006jb1a24"],"availability":"Available","catalogue":"eidc","dataFormat":["Comma-separated values (CSV)"],"description":"The data nitrogen gene data, soil biodiversity indices and microbial community composition, for three soil depths (0-15, 15-30 and 30-60 cm) from a three-cut silage plot trial located at three grassland sites within the UK collected between April 2016 and October 2016. The sites were Rothamsted Research at North Wyke in Devon, Bangor University at Henfaes Research Station in North Wales and Easter Bush in Scotland.\n\nAt each site measurements were taken from 16 plots, organised within a randomised complete block design where 4 plots did not receive fertilizers (controls), 4 plots received urea only, 4 plots received urea and urea-inhibitors, and 4 plots received ammonium-nitrate (Nitram). Fertiliser was applied three times and three cuts were performed, all parameters measured were following a fertiliser application. Samples were taken before the fertilizer additions, at peak growth and before the last silage cut.\n\nSoil chemical parameters were: soil nitrate, ammonium, dissolved organic carbon and nitrogen, amino acids and peptides, soil organic matter content as loss-on-ignition, pH, sodium, potassium, calcium, magnesium, permanganate oxdisable carbon citric acid extractable phosphorous, Olsen-P and total carbon, nitrogen and phosphorus. Soil biological measure were: microbial biomass carbon and nitrogen. Soil physico-chemical parameters were measured on the same samples and are available in a related dataset measured on the same soil samples and are presented in a separate dataset in the project data series (https://catalogue.ceh.ac.uk/id/7a87dde4-b54e-49b0-8751-1d59e8aebb90).\n\nMeasurements were undertaken by members of staff from the Centre of Ecology & Hydrology (Bangor, Edinburgh, Lancaster, Wallingford), Bangor University, School of Environment, Natural Resources & Geography and Rothamsted Research, Sustainable Agricultural Sciences, North Wyke.\n\nData was collected for the Newton Fund project \"UK-China Virtual Joint Centre for Improved Nitrogen Agronomy\". Funded by Biotechnology and Biological Sciences Research Council (BBSRC) and NERC -  Ref BB/N013468/1","documentType":"GEMINI_DOCUMENT","familyName":["Reinsch","Blaud","Abadie","Clark","Hirsch","Griffiths","Goodall","Carswell","Misselbrook","Shaw","Sánchez-Rodríguez","Chadwick","Jones","Cowan","Skiba"],"fullName":["Reinsch, S.","Blaud, A.","Abadie, M.","Clark, I.M.","Hirsch, P.R.","Griffiths, R.I.","Goodall, T.I.","Carswell, A.M.","Misselbrook, T.H.","Shaw, R.","Sánchez-Rodríguez, A.R.","Chadwick, D.R.","Jones, D.L.","Cowan, N.J.","Skiba, U."],"funder":["Biotechnology and Biological Sciences Research Council"],"givenName":["S.","A.","M.","I.M.","P.R.","R.I.","T.I.","A.M.","T.H.","R.","A.R.","D.R.","D.L.","N.J.","U."],"grant":["BB/N013468/1"],"identifier":"59f81d41-a789-4c5c-8ab8-36baa7ac2c55","incomingCitationCount":0,"keyword":["CINAg","Centre for improved Nitrogen agronomy","Soil","Urease Inhibitor","Soil properties","grassland","nitrogen","fertiliser","soil"],"keywordsOther":["Urease Inhibitor","Soil properties","grassland","nitrogen","fertiliser","soil"],"keywordsProject":["CINAg","Centre for improved Nitrogen agronomy"],"keywordsTheme":["Soil"],"licence":"Open government licence","lineage":"Soil samples were taken by members of the sites. At North Wyke and Henfaes farm, staff from CEH Bangor helped taking the samples. Two sets of soil cores were taken. One was sent in cooling boxes on ice to CEH Bangor for measuring soil physicochemical properties. The second set was sent to Rothamsted Research Harpenden for the analysis of nitrogen genes and microbial community composition (related dataset). The topsoil 0-15 cm were taken with the same type of corer across the sites, samples below 15 cm soil depth were sampled with corers available at the sites. Samples were wet sieved to 5 mm by staff from Bangor University and CEH Bangor. Subsamples of these were analysed for physicochemical and biological parameters.\n\nDNA extractions were performed by Rothamsted Research Harpenden. Extracts were analyzed for nitrogen genes at Rothamsted Research in Harpenden. Extracts were also sent to CEH Wallingford for analyses of Operational Taxonomic Units (OTU's).\n\nSoil community DNA was extracted from 0.25 gams soil using the MoBio DNA PowerSoil DNA isolation kit (Mo Bio Laboratories, Inc. Carlsbad, CA), following manufacturer’s protocol. Extracted DNA was quantified by fluorometer Qubit® 2.0 dsDNA BR Assay Kit (Thermo Fisher Scientific) and quality checked by nanodrop (Thermo Fisher Scientific).\n\nQuantitative-polymerase chain reaction (qPCR) protocols were as detailed in De Sosa et al. 2018.\n\nMicrobial nitrogen cycling gene abundance was investigated by qPCR targeting specific genes or genetic regions. Bacterial and archaeal communities were targeted via the 16S rRNA genes, while the fungal community abundance was targeted by the ITS region. The different genes involved in soil nitrogen cycling were investigated: \n•\tNitrogen fixation (nifH gene)\n•\tNitrification by targeting the ammonia oxidising bacteria (AOB) and archaea (AOA) (amoA gene)\n•\tDenitrifiers via the nitrite reductase (nirK and nirS genes) and the nitrous oxide reductase (nosZ genes clade I and II)\n•\tDegradation of urea was also assessed by targeting the ureC gene\n\nqPCR amplifications were performed in 10 μL volumes containing 5 μL of QuantiFast (Qiagen, Manchester, UK), 2.8 μL of nuclease-free water (Severn Biotech, Kidderminster, UK), 0.1 μL of each primer (1 μM) and 2 μL of template DNA at 5 ng μL−1, using a CFX384 Touch® Real-Time PCR Detection System (Bio-Rad, Hemel Hempstead, UK). \n\nStandards for each molecular target were obtained using a 10 fold serial dilution of PCR products amplified from an environmental reference DNA (also used as positive control) and purified by gel extraction using the Wizard® SV Gel and PCR Clean Up System (Promega, Southampton, UK) following the manufacturer's instruction and quantified by fluorometer Qubit® 2.0 dsDNA BR Assay Kit (Thermo FisherScientific). \n\nStandard curve template DNA and the negative/positive controls were amplified in triplicate. Amplification conditions for all qPCR assays consisted in 2 steps: first denaturation at 95 degrees Celsius for 5 minutes followed by 40 cycles at 95 degrees Celsius for 10 seconds and 60 degrees Celsius for 30 seconds that included annealing, elongation and reading. Each amplification was followed by melting curve (increase in temperature from 60 degrees Celsius to 95 degrees Celsius, with a reading every 0.5degrees Celsius) to assess the specificity of each assay.\n\nThe efficiency of the qPCR varied between 85.2 percent and 95.9 percent (except nozII 74.4 percent, amplicon 746 bp long), and R2 between 0.987 and 0.999. The melting curves showed specificity for all the genes, except as expected for the fungal Internal Transcribed Spacer (ITS), that showed the amplification of products of different lengths, due to the variability in length of the ITS region between different fungal taxa (Manter and Vivanco, 2007).\n\nMicrobial diversity indices:\nExtracted DNA was supplied from Rothamsted Research in Harpenden (see section 5.4.13 in main supporting document) for amplicon sequencing at the facilities of the Molecular Ecology Group, Centre for Ecology & Hydrology, Wallingford. <50 μL volumes of DNA extract were supplied in 96-well plate format, these were subject to:\n•\t16S rRNA amplicon sequencing as per Kozich 2013 (Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform), utilising V3-V4 region primers\n•\tITS rRNA amplicon sequencing employing the Kozich 2013 strategy, utilizing ITS primers by Ihrmark 2012 (New primers to amplify the fungal ITS2 region--evaluation by 454-sequencing of artificial and natural communities)\n\nAmplicons were sequenced on Illumina MiSeq platform with V3 chemistry. Resulting sequences were demultiplexed using Illumina Basespace. 16S amplicons were processed using in-house developed pipelines for quality filtering, merging and taxonomic assignment (GreenGenes database) of operational taxonomic units (OTUs). ITS amplicons were processed using the R package Dada2 for quality filtering, merging. \nR package Vegan was used to analyse amplicon sequences. Bacterial and fungal Shannon’s Index diversity scores for each sample were produced from rarefied data using diversity function. Species richness for each sample were similarly produced using ‘specnumber’ function.\n\nBacterial phylum level frequencies were produced from taxonomically identified OTU sequences (using Greengenes database), these values are a percentage of the rarefied total.\n\nAll results were entered into CSV spreadsheet.","locations":["POLYGON((-4.022 50.767, -4.022 55.873, -3.191 55.873, -3.191 50.767, -4.022 50.767))"],"metadataDate":"2026-06-29T16:59:02.000Z","orcid":["https://orcid.org/0000-0003-4649-0677","https://orcid.org/0000-0003-0860-8080","https://orcid.org/0000-0002-1589-5420","https://orcid.org/0000-0002-3341-4547","https://orcid.org/0000-0003-0580-6698","https://orcid.org/0000-0002-4594-3606","https://orcid.org/0000-0001-8734-2035","https://orcid.org/0000-0002-1482-4209","https://orcid.org/0000-0001-8659-6092"],"organisation":["UK Centre for Ecology & Hydrology","Rothamsted Research","Centre for Ecology & Hydrology","Bangor University","NERC EDS Environmental Information Data Centre","NERC Environmental Information Data Centre"],"publicationDate":"2019-04-24T00:00:00.000Z","recordType":"Dataset","resourceIdentifier":["https://catalogue.ceh.ac.uk/id/59f81d41-a789-4c5c-8ab8-36baa7ac2c55","10.5285/59f81d41-a789-4c5c-8ab8-36baa7ac2c55","doi:10.5285/59f81d41-a789-4c5c-8ab8-36baa7ac2c55"],"resourceType":"Dataset","ror":["https://ror.org/00pggkr55","https://ror.org/0347fy350","https://ror.org/006jb1a24","https://ror.org/04xw4m193"],"shortenedDescription":"The data nitrogen gene data, soil biodiversity indices and microbial community composition, for three soil depths (0-15, 15-30 and 30-60 cm) from a three-cut silage plot trial located at three grassland sites within the UK collected between April 2016 and October 2016.…","state":"published","supplementalDescription":["Manter, D. K., & Vivanco, J. M. (2007). Use of the ITS primers, ITS1F and ITS4, to characterize fungal abundance and diversity in mixed-template samples by qPCR and length heterogeneity analysis. Journal of Microbiological Methods, 71(1), 7–14.","Kozich, J.J., Westcott, S.L., Baxter, N.T., Highlander, S.K., & Schloss, P.D. (2013). Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform. Applied and Environmental Microbiology, 79(17), 5112–5120.","de Sosa, L.L., Glanville, H.C., Marshall, M.R., Schnepf, A., Cooper, D.M., Hill, P.W., … Jones, D.L. (2018). Stoichiometric constraints on the microbial processing of carbon with soil depth along a riparian hillslope. Biology and Fertility of Soils, 54(8), 949–963. "],"supplementalName":["Kozich 2013 ","GreenGenes database","The UK-China Virtual Joint Centre for Improved Nitrogen Agronomy (CINAG)"],"title":"Soil biological properties from an inorganic fertiliser (grass trial) experiment at North Wyke, Henfaes Farm and Easter Bush, UK (2016)","topic":["0/Soil/"],"version":1.0,"view":["public","danor"]},{"authorAffiliation":["Forest Research","University of Surrey"],"authorFamilyName":["Moseley","Marcu","Marzano","O'Brien"],"authorFullName":["Moseley, D.","Marcu, A.","Marzano, M.","O'Brien, L."],"authorGivenName":["Darren","Afrodita","Mariella","Liz"],"authorRor":["https://ror.org/03wcc3744","https://ror.org/00ks66431"],"availability":"Available","catalogue":"eidc","dataFormat":["Microsoft Excel","Shapefile"],"description":"This dataset consists of tick sampling and microclimate data from Exmoor, Richmond and New Forest study sites; as well as ARCGIS risk maps that model tick abundance driven by climate surfaces and host abundance. Tick sampling data (91 files, each representing a day of sampling) indicate tick abundance (distinguishing larvae, nymphs, adult males and adult females), vegetation height, soil moisture, temperature and relative humidity. Static risk map files indicate modeled tick abundance: 251 landcover files for the three sites, as well as 36 ArcView map files. The study is part of the NERC Rural Economy and Land Use (RELU) programme. Many people take pleasure from activities in forests and wild lands in the UK and others are being encouraged to participate. Unfortunately, there are risks and one of the most insidious is the possibility (albeit tiny) of acquiring a disease from wild animals; for example, ticks can be vectors of the bacterial infection leading to Lyme Disease. Both diagnosis and treatment can be problematic so prevention of acquiring such disease is highly desirable. Surprisingly little is known about how best to warn countryside users about the potential for disease without scaring them away or spoiling their enjoyment. Answering such questions was the goal of this project, and required the integration of a diverse set of scientific skills, and an understanding of the views of those who manage countryside, those who have contracted zoonotic diseases and those who access the land. This project combined knowledge from three strands of work, namely risk assessment, risk perception and communication, and scenario analysis. The study sites were selected to provide a range of environmental conditions and countryside use. Peri-urban parkland, accessible lowland forest and heath and remote upland forest were chosen as represented by Richmond Park on the fringe of Greater London, the New Forest in Southern England, and Exmoor in South West England. The following additional data from this same research project are available at the UK Data Archive under study number 6892 (see online resources): Lyme disease risk perception data resulting from tick imagery vignette experiments, Lyme disease patient interviews and surveys, residents and countryside staff focus groups, forest manager interviews, and multiple scoring procedures of animal social representation; as well as Lyme and tick risk communication data resulting from interviews with organisations and content analysis of risk warning information leaflets, Further documentation for this study may be found through the RELU Knowledge Portal and the project's ESRC funding award web page (see Supplemental).","documentType":"GEMINI_DOCUMENT","familyName":["Moseley","Quine","Randolph","Uzell","Barnett","Marcu","Marzano","O'Brien"],"fullName":["Moseley, D.","Quine, C.","Randolph, S.","Uzell, D.","Barnett, J.","Marcu, A.","Marzano, M.","O'Brien, L."],"funder":["Economic and Social Research Council"],"givenName":["Darren","Chris","Sarah","David","Julie","Afrodita","Mariella","Liz"],"grant":["RES-229-25-0007"],"identifier":"ac9175da-921c-4a46-a6bb-b31d73eab8a6","incomingCitationCount":0,"keyword":["Rural Economy and Land Use Programme (RELU)","Assessing and communicating animal disease risks for countryside users"],"keywordsOther":["Assessing and communicating animal disease risks for countryside users"],"keywordsProject":["Rural Economy and Land Use Programme (RELU)"],"licence":"Non-Open government licence","lineage":"Research funded by Economic and Social Research Council, Natural Environment Research Council and Biotechnology and Biological Sciences Research Council. Award Number: RES-229-25-0007 Tick sampling data (91 files, each representing a day of sampling) indicate tick abundance (distinguishing larvae, nymphs, adult males and adult females), vegetation height, soil moisture, temperature and relative humidity. Static risk map files indicate modeled tick abundance: 251 landcover files for the three sites, as well as 36 ArcView map files.","locations":["POLYGON((-3.671 50.861, -3.671 51.44, -0.278 51.44, -0.278 50.861, -3.671 50.861))"],"metadataDate":"2026-06-29T15:34:03.000Z","organisation":["Forest Research","Forest Research. Centre for Human and Ecological Sciences","University of Oxford","University of Surrey","Brunel University","NERC EDS Environmental Information Data Centre"],"publicationDate":"2013-07-25T00:00:00.000Z","recordType":"Dataset","resourceIdentifier":["1374764152990","CEH:EIDC::1374764152990","https://catalogue.ceh.ac.uk/id/ac9175da-921c-4a46-a6bb-b31d73eab8a6"],"resourceType":"Dataset","ror":["https://ror.org/03wcc3744","https://ror.org/052gg0110","https://ror.org/00ks66431","https://ror.org/04xw4m193"],"shortenedDescription":"This dataset consists of tick sampling and microclimate data from Exmoor, Richmond and New Forest study sites; as well as ARCGIS risk maps that model tick abundance driven by climate surfaces and host abundance. Tick sampling data (91 files, each representing a day…","state":"published","supplementalDescription":["Data collection record for social science data resulting from the same research project","Project information, publications and outputs accessible via the Relu Knowledge Portal"],"supplementalName":["Relu Knowledge Portal project page"],"title":"Tick ecology data and risk maps, 2007-2010 - RELU Assessing and communicating animal disease risks for countryside users","version":1.0,"view":["public","rstu"]},{"authorAffiliation":["Queen Mary University of London","Natural History Museum of Denmark","Universiti of Malaysia Sabah","Muséum national d'Histoire naturelle"],"authorFamilyName":["Drinkwater","Schnell","Bohmann","Bernard","Veron","Clare","Gilbert","Rossiter"],"authorFullName":["Drinkwater, R.","Schnell, I.D.","Bohmann, K.","Bernard, H.","Veron, G.","Clare, E.","Gilbert, T.P.","Rossiter, S.J."],"authorGivenName":["R.","I.D.","K.","H.","G.","E.","T.P.","S.J."],"authorOrcid":["https://orcid.org/0000-0001-6892-1664","https://orcid.org/0000-0002-0436-785X","https://orcid.org/0000-0001-7907-064X","https://orcid.org/0000-0003-4247-3976","https://orcid.org/0000-0002-6563-3365","https://orcid.org/0000-0002-5805-7195"],"authorRor":["https://ror.org/026zzn846","https://ror.org/040v70252","https://ror.org/03wkt5x30"],"availability":"Available","catalogue":"eidc","dataFormat":["Comma-separated values (CSV)"],"description":"This dataset contains the results from a metabarcoding study of terrestrial leech blood meals to detect differences in the diets of two leech species, Haemadipsa picta and Haemadipsa sumatrana. Mammal taxa were identified using metabarcoding of 16s rRNA and comparisons of operational taxonomic units (OTUs) to a curated reference database from NCBI (National Center for Biotechnology Information) GenBank. All leeches were collected from the Stability of Altered Forest Ecosystems project (SAFE; www.SAFEproject.net) as part of the NERC Human Modified tropical Forest Programme and the LOMBOK consortia (Land-use Options for Maintaining BiOdiversity & eKosystem functions). Leech samples were collected at different sites across a habitat gradient, to assess these invertebrates as molecular sampling tools for mammals. Individuals were pooled before amplicon sequencing with Illumina MiSeq 150-200bp x2. The resultant raw sequences were filtered and clustered at 97%, curated and then assigned to the reference database using BLAST and MEGAN programmes. ","documentType":"GEMINI_DOCUMENT","familyName":["Drinkwater","Schnell","Bohmann","Bernard","Veron","Clare","Gilbert","Rossiter"],"fullName":["Drinkwater, R.","Schnell, I.D.","Bohmann, K.","Bernard, H.","Veron, G.","Clare, E.","Gilbert, T.P.","Rossiter, S.J."],"funder":["Natural Environment Research Council"],"givenName":["Rosie","R.","I.D.","K.","H.","G.","E.","T.P.","S.J."],"grant":["NE/K016148/1"],"identifier":"3affed0d-fe6f-4916-89e3-e672639191e5","incomingCitationCount":0,"keyword":["Sabah","Borneo","Biodiversity","Haemadipsa picta","Haemadipsa sumatrana"],"keywordsOther":["Haemadipsa picta","Haemadipsa sumatrana"],"keywordsPlace":["Sabah","Borneo"],"keywordsTheme":["Biodiversity"],"licence":"Open government licence","lineage":"Individuals of both species were collected at 25m2 vegetation plots in the forest blocks (A-F, LFE, LF1-3 & VJR) at the SAFE project Sabah (corresponding to second order points). Plots are approximately 173m apart and all plots were sampled 4 times between February 2015 and June 2015. Sampling consists of searching the leaf litter and undergrowth for leeches for a fixed time of 20 minutes. Individuals are kept in RNALater to stabilise DNA until extraction. DNA extraction of pools of ten individual leeches was undertaken using a digestion buffer with modified QiaQuick (Qiagen) protocol and dual tagged PCR in triplicate reactions (details in Drinkwater et al. in review).  After screening samples for successful PCR amplification, a total of 41 samples of pooled Haemadipsa sumatrana and 49 pooled Haemadipsa picta from six sites remained for sequencing (B, F, D, LF, LFE, VJR). Amplicons were pooled for library build following Carøe et al, 2017) and sequencing with Illumina MiSeq 150-200bp x2. Sumaclust was used for OTU clustering (Mercier et al., 2013) and LULU was used for post-clustering filtering (Frøslev et al., 2017). This resulted in the final contingency table comparing mammal detections with leech pool. \n\nCarøe, C., Gopalakrishnan, S., Vinner, L., Mak, S. S. T., Sinding, M. H. S., Samaniego, J. A., Wales, N., Sicheritz-Ponten, M., Gilbert, M. T. P. (2017). Single-tube library preparation for degraded DNA. Methods in Ecology and Evolution, 9(2), 410-419. https://doi.org/10.1111/2041-210X.12871\n\nFrøslev, T. G., Kjøller, R., Bruun, H. H., Ejrnæs, R., Brunbjerg, A. K., Pietroni, C., & Hansen, A. J. (2017). Algorithm for post-clustering curation of DNA amplicon data yields reliable biodiversity estimates. Nature Communications, 8(1), 1188. https://doi.org/10.1038/s41467-017-01312-x\n\nMercier, C., Boyer, F., Bonin, A., & Coissac, E. (2013). SUMATRA and SUMACLUST: fast and exact comparison and clustering of sequences. In Programs and Abstracts of the SeqBio workshop (pp. 27-29).","locations":["POLYGON((113.75 4.182, 113.75 7.111, 119.353 7.111, 119.353 4.182, 113.75 4.182))"],"metadataDate":"2026-06-29T16:28:16.000Z","orcid":["https://orcid.org/0000-0001-6892-1664","https://orcid.org/0000-0002-0436-785X","https://orcid.org/0000-0001-7907-064X","https://orcid.org/0000-0003-4247-3976","https://orcid.org/0000-0002-6563-3365","https://orcid.org/0000-0002-5805-7195"],"organisation":["Queen Mary University of London","Natural History Museum of Denmark","Universiti of Malaysia Sabah","Muséum national d'Histoire naturelle","NERC EDS Environmental Information Data Centre","NERC Environmental Information Data Centre"],"publicationDate":"2018-08-08T00:00:00.000Z","recordType":"Dataset","resourceIdentifier":["https://catalogue.ceh.ac.uk/id/3affed0d-fe6f-4916-89e3-e672639191e5","10.5285/3affed0d-fe6f-4916-89e3-e672639191e5","doi:10.5285/3affed0d-fe6f-4916-89e3-e672639191e5"],"resourceType":"Dataset","ror":["https://ror.org/026zzn846","https://ror.org/040v70252","https://ror.org/03wkt5x30","https://ror.org/04xw4m193"],"shortenedDescription":"This dataset contains the results from a metabarcoding study of terrestrial leech blood meals to detect differences in the diets of two leech species, Haemadipsa picta and Haemadipsa sumatrana. Mammal taxa were identified using metabarcoding of 16s rRNA and comparisons…","state":"published","supplementalName":["SAFE project website"],"title":"Mammal occurrence identified from metabarcoding of two leech species from Sabah, Malaysia","topic":["0/Biodiversity/"],"version":1.0,"view":["public","phtr"]}],"rows":20,"url":"http://catalogue.ceh.ac.uk:443/eidc/documents?facet=grant%7C(BBS%20OR%20BB/N013468/1%20OR%20RES-229-25-0007%20OR%20NE/N016777/1%20OR%20NE/K016148/1%20OR%20NE/X012018/1)"}