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:12f2f235-8e18-40c7-9113-acb85de9edd7
dcterms:title "Phenotypic traits of Rhizobium leguminosarum TRX19 clones evolved in the presence of different lytic bacteriophages" ;
dcterms:identifier "https://catalogue.ceh.ac.uk/id/12f2f235-8e18-40c7-9113-acb85de9edd7","https://doi.org/10.5285/12f2f235-8e18-40c7-9113-acb85de9edd7" ;
dcterms:bibliographicCitation "Pain, C., Harrison, E. (2026). Phenotypic traits of Rhizobium leguminosarum TRX19 clones evolved in the presence of different lytic bacteriophages. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/12f2f235-8e18-40c7-9113-acb85de9edd7" ;
dcterms:description "This dataset comprises phenotypic data of Rhizobium leguminosarum TRX19 clones evolved in the presence or absence of lytic phages. The clones assayed were isolated from populations of R. leguminosarum which had been evolved in lab media for twenty 73hr 'transfers'. Clones were assessed for their resistance to the ancestral phages (reduction in bacterial growth), their ability to form symbioses (plant biomass, nitrogen content and nodule counts) and traits associated with colonisation (biofilm production, swimming and swarming motility and gelatinase activity). This experiment tests the hypothesis that evolution for phage resistance will drive the loss of symbiosis functions in rhizobia due to antagonistic pleiotropy." ;
dcterms:provenance [
a dcterms:ProvenanceStatement ;
rdfs:label "This dataset contains assay data from an evolution experiment assessing the impact of evolution with different lytic phages on the symbiotic performance of rhizobia. Briefly, strain Rhizobium leguminosarum TRX19 was evolved in lab media with 1 of 3 phages or in the absence of phage. Clones were isolated from evolved populations and screened for resistance, symbiotic function and phenotypes related to colonisation. Most of the data are raw values acquired from assays of the evolved clones. These include: growth with host plant - collecting plant biomass, nodule counts and nitrogen content via Isotope ratio mass spectrometry. Phenotypic analysis - biofilm production, swimming and swarming motility, growth rate in liquid broth and gelatinase assays Phage resistance data is presented as RBG - reduction in bacterial growth - generated as the ratio of the density (OD600) of cultures grown in a microtitre plate either with or without phages. The ratio of phage exposed to phage free bacterial growth indicates the severity of the infection."
] ;
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<http://onto.nerc.ac.uk/CEHMD/topic/19>,
"Bacteriophage",
"Rhizobia",
"Antagonistic coevolution",
<http://www.eionet.europa.eu/gemet/concept/8259> ;
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foaf:name "Ellie Harrison" ;
vcard:hasEmail "ellie.harrison@sheffield.ac.uk" ;
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foaf:name "Pain, C." ;
foaf:familyName "Pain" ;
foaf:givenName "Charly" ;
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skos:prefLabel "Evolutionary ecology"; rdfs:label "Evolutionary ecology"
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skos:prefLabel "symbiosis"; rdfs:label "symbiosis"
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