Operational taxonomic units for epilithon eukaryotes in the rivers Wolf and Tamar catchments
type: Dataset
citation: Griffiths, R., Edwards, F., Scarlett, P., Webb, G., Sarlej, R., Thomson, B. (2014). Operational taxonomic units for epilithon eukaryotes in the rivers Wolf and Tamar catchments. NERC Environmental Information Data Centre. (Dataset). https://doi.org/10.5285/18023db5-25d8-44f7-b291-61869f937367
description: This dataset contains operational taxonomic units for epilithon eukaryotes (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wolf River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.
is accessible for free: true
date created: 2010-01-01
date published: 2014-05-06
keywords: Environmental monitoring facilities
keywords: microbiology
keywords: South West project
keywords: Species distribution
keywords: Tellus
keywords: Biodiversity
keywords: genetics
keywords: hydrography
keywords: soil
creator: Griffiths, R.
creator: Edwards, F.
creator: Scarlett, P.
creator: Webb, G.
creator: Sarlej, R.
creator: Thomson, B.
temporal coverage: 2013-10-01/2014-03-20
spatial coverage: bbox0
license: Open Government Licence v3
distribution: distribution0
publisher: NERC EDS Environmental Information Data Centre
provider: NERC EDS Environmental Information Data Centre
included in data catalog: Environmental Information Data Centre

Biodiversity
type: DefinedTerm

genetics
type: DefinedTerm
in defined term set: https://www.eionet.europa.eu/gemet/

hydrography
type: DefinedTerm
in defined term set: https://www.eionet.europa.eu/gemet/

microbiology
type: DefinedTerm
in defined term set: https://www.eionet.europa.eu/gemet/

soil
type: DefinedTerm
in defined term set: https://www.eionet.europa.eu/gemet/

Environmental Information Data Centre
type: DataCatalog
alternate name: EIDC

NERC EDS Environmental Information Data Centre
type: Organization

Open Government Licence v3
type: CreativeWork
alternate name: OGL-UK-3.0

geoshape0
type: GeoShape
box: -4.378 50.351, -3.906 50.785

Griffiths, R.
type: Person
family name: Griffiths
given name: R.
affiliation: UK Centre for Ecology & Hydrology

Edwards, F.
type: Person
family name: Edwards
given name: F.
affiliation: UK Centre for Ecology & Hydrology

Scarlett, P.
type: Person
family name: Scarlett
given name: P.
affiliation: UK Centre for Ecology & Hydrology

Webb, G.
type: Person
family name: Webb
given name: G.
affiliation: UK Centre for Ecology & Hydrology

Sarlej, R.
type: Person
family name: Sarlej
given name: R.
affiliation: UK Centre for Ecology & Hydrology

Thomson, B.
type: Person
family name: Thomson
given name: B.
affiliation: UK Centre for Ecology & Hydrology

distribution0
type: DataDownload
encoding format: application/zip

UK Centre for Ecology & Hydrology
type: Organization