{"accessLimitation":{"value":"noLimitations","availability":"Available","description":"Access to the resource is not subject to any access limitations and is available to all users without restriction.","uri":"http://vocab.nerc.ac.uk/collection/N07/current/UNRS/"},"authors":[{"familyName":"MacDonald","givenName":"H.C.","organisationName":"Cardiff University","organisationIdentifier":"https://ror.org/03kk7td41","role":"author","email":"Hannah.c.burton@gmail.com","address":{"deliveryPoint":"Sir Martin Evans Building, Museum Avenue","city":"Cardiff","country":"United Kingdom"},"fullName":"MacDonald, H.C."},{"familyName":"Bruford","givenName":"M.W.","organisationName":"Cardiff University","organisationIdentifier":"https://ror.org/03kk7td41","role":"author","email":"BrufordMW@cardiff.ac.uk","address":{"deliveryPoint":"Sir Martin Evans Building, Museum Avenue","city":"Cardiff","postalCode":"CF10 3AX","country":"United Kingdom"},"fullName":"Bruford, M.W."},{"familyName":"Ormerod","givenName":"S.","organisationName":"Cardiff University","organisationIdentifier":"https://ror.org/03kk7td41","role":"author","email":"Ormerod@cardiff.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-8174-302X","address":{"deliveryPoint":"Sir Martin Evans Building, Museum Avenue","city":"Cardiff","postalCode":"CF10 3AX","country":"United Kingdom"},"fullName":"Ormerod, S."},{"familyName":"Jones","givenName":"T.H.","organisationName":"Cardiff University","organisationIdentifier":"https://ror.org/03kk7td41","role":"author","email":"JonesTH@cardiff.ac.uk","address":{"deliveryPoint":"Sir Martin Evans Building, Museum Avenue","city":"Cardiff","postalCode":"CF10 3AX","country":"United Kingdom"},"fullName":"Jones, T.H."}],"availability":"Available","boundingBoxes":[{"westBoundLongitude":-4.073,"eastBoundLongitude":-3.08,"southBoundLatitude":51.921,"northBoundLatitude":53.123,"bounds":"{\"type\": \"Feature\",      \"properties\": {},      \"geometry\": {        \"type\": \"Polygon\",        \"coordinates\": [[[-4.073, 51.921], [-4.073, 53.123], [-3.08, 53.123], [-3.08, 51.921], [-4.073, 51.921]]]      }}","coordinates":"[[[-4.073, 51.921], [-4.073, 53.123], [-3.08, 53.123], [-3.08, 51.921], [-4.073, 51.921]]]"}],"citation":{"authors":["MacDonald, H.C.","Bruford, M.W.","Ormerod, S.","Jones, T.H."],"bibtex":"https://catalogue.ceh.ac.uk/documents/27da4400-9835-4241-9df9-a3b598f8d274/citation?format=bib","day":20,"doi":"10.5285/27da4400-9835-4241-9df9-a3b598f8d274","month":9,"publisher":"NERC Environmental Information Data Centre","resourceTypeGeneral":"dataset","ris":"https://catalogue.ceh.ac.uk/documents/27da4400-9835-4241-9df9-a3b598f8d274/citation?format=ris","title":"Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013)","url":"https://doi.org/10.5285/27da4400-9835-4241-9df9-a3b598f8d274","year":2017},"custodians":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"}],"datasetReferenceDate":{"creationDate":"2013-06-15","publicationDate":"2017-09-20"},"description":"The resource consists of genotype data (genetic constitution of an individual organism) for three freshwater macroinvertebrate species, Amphinemura sulcicollis, Isoperla grammatica and Baetis rhodani collected at sites in upland Wales during May 2012 and May 2013. New nucleotide tandem repeats in DNA sequences i.e. Novel microsatellites were developed for this project so that genetic analysis could be carried out within the three species. Extra data on how microsatellite scores were grouped i.e. binned into unique alleles is provided, to aid replication. The data was generated for Hannah Macdonald's PhD thesis: to study genetic structure and diversity, demographic species history and resilience in freshwater ecosystems. \n\nThe work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was funded by the Natural Environment Research Council (NERC) under the Biodiversity and Ecosystem Service Sustainability (BESS) programme.","distributionFormats":[{"name":"Comma-separated values (CSV)","type":"text/csv","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"funding":[{"funderName":"Natural Environment Research Council","funderIdentifier":"https://ror.org/02b5d8509","awardTitle":"Diversity in Upland Rivers for Ecosystem Service Sustainability - DURESS","awardNumber":"NE/J014818/1","awardURI":"http://gtr.ukri.org/projects?ref=NE/J014818/1","orcid":false,"ror":true}],"id":"27da4400-9835-4241-9df9-a3b598f8d274","incomingCitationCount":0,"infoLinks":[{"url":"https://data-package.ceh.ac.uk/sd/27da4400-9835-4241-9df9-a3b598f8d274.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"inspireThemes":[{"theme":"Habitats and Biotopes","uri":"http://inspire.ec.europa.eu/theme/hb"}],"keywordsOther":[{"value":"Amphinemura sulcicollis","uri":"http://www.wikidata.org/entity/Q10408946"},{"value":"Isoperla grammatica","uri":"http://www.wikidata.org/entity/Q6385304"},{"value":"Baetis rhodani","uri":"http://www.wikidata.org/entity/Q732867"}],"keywordsProject":[{"value":"Diversity of Upland Rivers for Ecosystem Service Sustainability (DURESS)"}],"licences":[{"value":"This resource is made available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}],"lineage":"Samples of each invertebrate were collected by kicksampling in May in 2012 and 2013, samples were sorted and identified to genus level on site and stored in absolute ethanol. In the laboratory each larva was identified to species level using Elliott et al. (1988) and Hynes (1977). DNA was then extracted from tissue samples (see supporting information for extraction methods). Baetis rhodani samples contained cryptic species which had to be removed before genotyping, no cryptic species were identified for Amphinemura sulcicollis or Isoperla grammatica. Samples were sent for fragment analysis (Dundee Biosciences, UK) using ROX500 as the internal size standard. Fragment analysis products were visualised and scored using Genemarker v 1.91 (Holland and Parson 2011). These results were downloaded from Genemarker and allele sizes were binned by hand using the 'Binning' information (provided in the metadata) in an Excel spreadsheet. The data were then complied into master Excel spreadsheets for each invertebrate. The data were then exported as comma separated value files for ingestion into the EIDC.","metadataDate":"2026-06-29T16:32:10","notGEMINI":false,"onlineResources":[{"url":"https://data-package.ceh.ac.uk/data/27da4400-9835-4241-9df9-a3b598f8d274","name":"Download the data","description":"Download a copy of this data","function":"download","type":"OTHER"},{"url":"https://data-package.ceh.ac.uk/sd/27da4400-9835-4241-9df9-a3b598f8d274.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"pointsOfContact":[{"familyName":"MacDonald","givenName":"Hannah","organisationName":"Cardiff University","organisationIdentifier":"https://ror.org/03kk7td41","role":"pointOfContact","email":"Hannah.c.burton@gmail.com","address":{"deliveryPoint":"Sir Martin Evans Building, Museum Avenue","city":"Cardiff","postalCode":"CF10 3AX","country":"United Kingdom"},"fullName":"MacDonald, H.","pointOfContact":"Cardiff University"}],"publicationDate":"2017-09-20T00:00:00.000Z","publishers":[{"organisationName":"NERC Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"}],"relationships":[{"relation":"http://purl.org/dc/terms/isPartOf","target":"af32b249-e1a5-4c03-8fe2-4fb0e4f474bd"}],"resourceIdentifiers":[{"code":"https://catalogue.ceh.ac.uk/id/27da4400-9835-4241-9df9-a3b598f8d274"},{"code":"10.5285/27da4400-9835-4241-9df9-a3b598f8d274","codeSpace":"doi"}],"resourceMaintenance":[{"frequencyOfUpdate":"notPlanned"}],"resourceType":{"value":"dataset"},"responsibleParties":[{"familyName":"MacDonald","givenName":"Hannah","organisationName":"Cardiff University","organisationIdentifier":"https://ror.org/03kk7td41","role":"pointOfContact","email":"Hannah.c.burton@gmail.com","address":{"deliveryPoint":"Sir Martin Evans Building, Museum Avenue","city":"Cardiff","postalCode":"CF10 3AX","country":"United Kingdom"},"fullName":"MacDonald, H.","pointOfContact":"Cardiff University"},{"familyName":"MacDonald","givenName":"H.C.","organisationName":"Cardiff University","organisationIdentifier":"https://ror.org/03kk7td41","role":"author","email":"Hannah.c.burton@gmail.com","address":{"deliveryPoint":"Sir Martin Evans Building, Museum Avenue","city":"Cardiff","country":"United Kingdom"},"fullName":"MacDonald, H.C."},{"familyName":"Bruford","givenName":"M.W.","organisationName":"Cardiff University","organisationIdentifier":"https://ror.org/03kk7td41","role":"author","email":"BrufordMW@cardiff.ac.uk","address":{"deliveryPoint":"Sir Martin Evans Building, Museum Avenue","city":"Cardiff","postalCode":"CF10 3AX","country":"United Kingdom"},"fullName":"Bruford, M.W."},{"familyName":"Ormerod","givenName":"S.","organisationName":"Cardiff University","organisationIdentifier":"https://ror.org/03kk7td41","role":"author","email":"Ormerod@cardiff.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-8174-302X","address":{"deliveryPoint":"Sir Martin Evans Building, Museum Avenue","city":"Cardiff","postalCode":"CF10 3AX","country":"United Kingdom"},"fullName":"Ormerod, S."},{"familyName":"Jones","givenName":"T.H.","organisationName":"Cardiff University","organisationIdentifier":"https://ror.org/03kk7td41","role":"author","email":"JonesTH@cardiff.ac.uk","address":{"deliveryPoint":"Sir Martin Evans Building, Museum Avenue","city":"Cardiff","postalCode":"CF10 3AX","country":"United Kingdom"},"fullName":"Jones, T.H."},{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"},{"organisationName":"NERC Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"},{"organisationName":"Cardiff University","organisationIdentifier":"https://ror.org/03kk7td41","role":"rightsHolder"}],"rightsHolders":[{"organisationName":"Cardiff University","organisationIdentifier":"https://ror.org/03kk7td41","role":"rightsHolder"}],"spatialReferenceSystems":[{"code":"http://www.opengis.net/def/crs/EPSG/0/27700","title":"OSGB 1936 / British National Grid"}],"spatialRepresentationTypes":["textTable"],"temporalExtents":[{"begin":"2012-06-15","end":"2013-06-15"}],"title":"Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013)","topicCategories":[{"value":"biota","uri":"http://inspire.ec.europa.eu/metadata-codelist/TopicCategory/biota"}],"type":"dataset","uri":"https://catalogue.ceh.ac.uk/id/27da4400-9835-4241-9df9-a3b598f8d274","useConstraints":[{"value":"This resource is made available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"},{"value":"© Cardiff University","code":"copyright"}]}