Operational taxonomic units for soil bacteria in the Wolf and Tamar catchments
identifier: CEH:EIDC:/1395928628829
bibliographic citation: Griffiths, R., Pywell, R.F., Peyton, J., Nuttall, P., Sarlej, R., Thomson, B. (2014). Operational taxonomic units for soil bacteria in the Wolf and Tamar catchments. NERC Environmental Information Data Centre. https://doi.org/10.5285/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b
description: This dataset contains operational taxonomic units for soil bacteria collected from various land use categories in the Wolf and Tamar catchments in South west England. A range of soils were targeted from the Tamar region comprising a range of land uses. Approximate location of sampling sites was determined from maps to provide good spatial coverage of the catchment. Exact sites were determined in the field, considering accessibility and other logistic, and soils taken.
provenance: ProvenanceStatement
  label: Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.
spatial: Location
temporal: PeriodOfTime
  start date: 2013-10-01
  end date: 2014-03-20
publisher: NERC Environmental Information Data Centre
subject: Tellus
subject: South West project
subject: microbiology
subject: genetic information
type: Dataset
available: 2014-05-06
landing page: https://doi.org/10.5285/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b
license: OGL-UK-3.0.ttl
rights: RightsStatement
  copyright notice: copyright UK Centre for Ecology & Hydrology
access rights: UNRS
distribution:
  access URL: https://data-package.ceh.ac.uk/data/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b
  license: OGL-UK-3.0.ttl
  rights: RightsStatement
    copyright notice: copyright UK Centre for Ecology & Hydrology
  access rights: UNRS
  format: Comma-separated values (CSV)
creator: Pywell, R.F.
creator: Peyton, J.
language: en

Griffiths, R.
type: Person
family name: Griffiths
given name: Robert
member: UK Centre for Ecology & Hydrology

NERC Environmental Information Data Centre
type: Organization

Griffiths, R.
type: Person
family name: Griffiths
given name: R.
member: UK Centre for Ecology & Hydrology

Pywell, R.F.
type: Person
family name: Pywell
given name: R.F.
member: UK Centre for Ecology & Hydrology

Peyton, J.
type: Person
family name: Peyton
given name: J.
member: UK Centre for Ecology & Hydrology

Nuttall, P.
type: Person
family name: Nuttall
given name: P.
member: UK Centre for Ecology & Hydrology

Sarlej, R.
type: Person
family name: Sarlej
given name: R.
member: UK Centre for Ecology & Hydrology

Thomson, B.
type: Person
family name: Thomson
given name: B.
member: UK Centre for Ecology & Hydrology

microbiology
type: Concept

genetic information
type: Concept

UK Centre for Ecology & Hydrology
type: Organization