{"accessLimitation":{"value":"superseded","availability":"Superseded","description":"Access to the resource is limited to users who have been authorised by the data custodian or data owner in accordance with applicable policies, agreements, or licensing conditions","uri":"http://vocab.nerc.ac.uk/collection/N07/current/RAUT/"},"authors":[{"familyName":"Leitão","givenName":"A.B.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"ac2016@cam.ac.uk","nameIdentifier":"https://orcid.org/0000-0001-9004-1831","fullName":"Leitão, A.B."},{"familyName":"Ramesh","givenName":"A.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"ar885@cam.ac.uk","nameIdentifier":"https://orcid.org/0000-0003-0050-3516","fullName":"Ramesh, A."},{"familyName":"Day","givenName":"J.P.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"jd642@cam.ac.uk","fullName":"Day, J.P."},{"familyName":"Hanna","givenName":"N.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"naeeh2@cam.ac.uk","fullName":"Hanna, N."},{"familyName":"Devi","givenName":"A.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"aarathidevi@hotmail.com","fullName":"Devi, A."},{"familyName":"Hayes","givenName":"M.P.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"mph51@cam.ac.uk","fullName":"Hayes, M.P."},{"familyName":"Jiggins","givenName":"F.M.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"fmj1001@cam.ac.uk","nameIdentifier":"https://orcid.org/0000-0001-7470-8157","fullName":"Jiggins, F.M."}],"availability":"Superseded","citation":{"authors":["Leitão, A.B.","Ramesh, A.","Day, J.P.","Hanna, N.","Devi, A.","Hayes, M.P.","Jiggins, F.M."],"bibtex":"https://catalogue.ceh.ac.uk/documents/2998b066-6a35-4e4f-ae39-16838781856b/citation?format=bib","day":1,"doi":"10.5285/2998b066-6a35-4e4f-ae39-16838781856b","month":10,"publisher":"NERC EDS Environmental Information Data Centre","resourceTypeGeneral":"nonGeographicDataset","ris":"https://catalogue.ceh.ac.uk/documents/2998b066-6a35-4e4f-ae39-16838781856b/citation?format=ris","title":"Changes in gene expression and encapsulation in Drosophila melanogaster caused by exposure to parasitoid wasps","url":"https://doi.org/10.5285/2998b066-6a35-4e4f-ae39-16838781856b","year":2021},"custodians":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"}],"datasetReferenceDate":{"publicationDate":"2021-10-01","supersededDate":"2022-04-04"},"description":"Data consists of gene expression estimates and encapsulation rates in wild caught Drosophila melanogaster larvae following exposure to different treatments. Treatments include injection with wasp homogenate, injection with oil and no injection. Also provided are functional enrichment categories for differentially expressed genes and library generation and read mapping metrics. \n\nThe data were produced under the grant: NE/P00184X/1","distributionFormats":[{"name":"Comma-separated values (CSV)","type":"text/csv","version":"unknown"},{"name":"Plain text","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"funding":[{"funderName":"Natural Environment Research Council","funderIdentifier":"https://ror.org/02b5d8509","awardTitle":"Why does Drosophila vary in susceptibility to parasitoid wasps?","awardNumber":"NE/P00184X/1","awardURI":"https://gtr.ukri.org/projects?ref=NE/P00184X/1","orcid":false,"ror":true}],"id":"2998b066-6a35-4e4f-ae39-16838781856b","incomingCitationCount":0,"keywordsOther":[{"value":"RNA-sequencing"},{"value":"Drosophila melanogaster","uri":"http://www.wikidata.org/entity/Q130888"},{"value":"Leptopilina boulardi","uri":"http://www.wikidata.org/entity/Q14636778"},{"value":"parasitoid wasp"},{"value":"differential gene expression"},{"value":"fat body"},{"value":"encapsulation"},{"value":"haemocyte"},{"value":"Evolutionary genetics"},{"value":"insect genomics"},{"value":"parasite","uri":"http://www.eionet.europa.eu/gemet/concept/6035"},{"value":"encapsulation","uri":"http://www.eionet.europa.eu/gemet/concept/2693"},{"value":"genetics","uri":"http://www.eionet.europa.eu/gemet/concept/3633"},{"value":"insect","uri":"http://www.eionet.europa.eu/gemet/concept/4358"}],"licences":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}],"lineage":"Drosophila melanogaster larvae obtained from a wild caught population were exposed injected with wasp homogenate, oil or no manipulation was made. Encapsulation rates were measured in each case in the fly larvae. For RNA-sequencing, hemocyte and fat body tissues were isolated from third instar larvae 24 hours post experimental manipulation. RNA was isolated for sequencing. Sequenced reads were trimmed, mapped to the dm6 genome, number of reads mapping to each gene was counted, differential expression tests were applied and functional enrichment was done.","metadataDate":"2026-06-29T15:48:49","notGEMINI":false,"pointsOfContact":[{"familyName":"Ramesh","givenName":"A.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"pointOfContact","email":"ar885@cam.ac.uk","nameIdentifier":"https://orcid.org/0000-0003-0050-3516","fullName":"Ramesh, A.","pointOfContact":"University of Cambridge"}],"publicationDate":"2021-10-01T00:00:00.000Z","publishers":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"}],"reasonChanged":"We relaxed the threshold for filtering out lowly expressed genes. Here we include genes that had counts per million > 2 in at least 4 out of 12 libraries, performed separately for fat body and hemocytes. This replaces the more stringent filter of counts per million > 10 in at least 4 libraries that was applied previously.","resourceIdentifiers":[{"code":"https://catalogue.ceh.ac.uk/id/2998b066-6a35-4e4f-ae39-16838781856b"},{"code":"10.5285/2998b066-6a35-4e4f-ae39-16838781856b","codeSpace":"doi"}],"resourceType":{"value":"nonGeographicDataset"},"responsibleParties":[{"familyName":"Leitão","givenName":"A.B.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"ac2016@cam.ac.uk","nameIdentifier":"https://orcid.org/0000-0001-9004-1831","fullName":"Leitão, A.B."},{"familyName":"Ramesh","givenName":"A.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"ar885@cam.ac.uk","nameIdentifier":"https://orcid.org/0000-0003-0050-3516","fullName":"Ramesh, A."},{"familyName":"Day","givenName":"J.P.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"jd642@cam.ac.uk","fullName":"Day, J.P."},{"familyName":"Hanna","givenName":"N.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"naeeh2@cam.ac.uk","fullName":"Hanna, N."},{"familyName":"Devi","givenName":"A.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"aarathidevi@hotmail.com","fullName":"Devi, A."},{"familyName":"Hayes","givenName":"M.P.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"mph51@cam.ac.uk","fullName":"Hayes, M.P."},{"familyName":"Jiggins","givenName":"F.M.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"author","email":"fmj1001@cam.ac.uk","nameIdentifier":"https://orcid.org/0000-0001-7470-8157","fullName":"Jiggins, F.M."},{"familyName":"Ramesh","givenName":"A.","organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"pointOfContact","email":"ar885@cam.ac.uk","nameIdentifier":"https://orcid.org/0000-0003-0050-3516","fullName":"Ramesh, A.","pointOfContact":"University of Cambridge"},{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"},{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"},{"organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"rightsHolder","email":"ar885@cam.ac.uk"}],"rightsHolders":[{"organisationName":"University of Cambridge","organisationIdentifier":"https://ror.org/013meh722","role":"rightsHolder","email":"ar885@cam.ac.uk"}],"temporalExtents":[{"begin":"2017-10-01","end":"2017-10-31"}],"title":"Changes in gene expression and encapsulation in Drosophila melanogaster caused by exposure to parasitoid wasps","topicCategories":[{"value":"biota","uri":"http://inspire.ec.europa.eu/metadata-codelist/TopicCategory/biota"}],"type":"nonGeographicDataset","uri":"https://catalogue.ceh.ac.uk/id/2998b066-6a35-4e4f-ae39-16838781856b","useConstraints":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}]}