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:2ae5e8d3-be36-4517-b80c-c6b91792b769
dcterms:title "Hourly temperature, environmental parameter and Daphnia magna phenotypic and genetic change in mesocosms with manipulated experimental parameters, 2017-2019" ;
dcterms:identifier <https://catalogue.ceh.ac.uk/id/2ae5e8d3-be36-4517-b80c-c6b91792b769> ;
adms:identifier <https://doi.org/10.5285/2ae5e8d3-be36-4517-b80c-c6b91792b769> ;
dcterms:bibliographicCitation "Brunner, F.S., Reynolds, A., Price, S., White, N., Sadler, D.E., Wilson, I.W., Atkinson, D.A., Paterson, S., Plaistow, S.J. (2022). Hourly temperature, environmental parameter and Daphnia magna phenotypic and genetic change in mesocosms with manipulated experimental parameters, 2017-2019. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/2ae5e8d3-be36-4517-b80c-c6b91792b769" ;
dcterms:description "This dataset contains information about hourly temperature variation, phenotypic and genetic change, and change in environmental parameters in a two-year mesocosm study designed to tease apart the impact that phenotypic plasticity and genetic diversity have on rate of adaptation to experimental heatwaves. All data were collected between 2017 and 2019. Thermal data was collected continuously using data loggers. The frequency of natural heatwaves was manipulated using a programmable aquatic mesocosm facility using data collected from real heatwaves from 2006. Phenotypic evolution was tracked using intermittent common garden life-history studies while changes in clone frequency were determined using microsatellite markers to track changes in clone frequency in manipulated populations over two years. Experimental data on zooplankton community dynamics were monitored using intermittent depth integrated sampling of communities in each mesocosm over two years. The work was supported by the Natural Environment Research Council (Grant NE/N016017/1)." ;
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rdfs:label "Daphnia magna clones used in the experiment were isolated from Brown Moss in Shropshire (52°57'01'N 2°39'05'W) and Lake Ring in Denmark (55°57′51.83″N, 9°35′46.87″E) as hatched resting eggs. How plastic each clone is was assayed by rearing clones in common garden studies using four different environments. Forty-two mesocosms with different numbers of plastic and non-plastic Daphnia magna clones were established at Ness botanic gardens (53°16'20'N 3°02'34'W). Heatwave induction and hourly temperature data were managed using heating elements and temperature sensors at 50cm depth connected to Campbell Scientific CR6 Wi-Fi data loggers connected to a virtual server. Environmental parameter data were collected using hand-held meters every 2-weeks. Water chemistry data were collected every 3-months between 2017-2019 and analysed using a spectrophotometer (see supporting doc for details). The life-history and thermal tolerance of D. magna in mesocosms was assayed 5 times a year before and after heatwaves (September 2017 March 2018, June 2018, August 2018, and October 2018) by sampling animals from each mesocosm and rearing an offspring in common garden study. Clone-frequency changes were monitored in a subset of mesocosms at 8 timepoints between 2017-2019 using four microsatellite markers. Community dynamics in each mesocosm were assessed at 8-timepoints between 2017-2019 using depth integrated samples from four locations across a mesocosm for a total of 10L of water, which was then filtered through 100 micromitre sieves and stored in 15% glycerol at 20 oC prior to counting. All data were checked, verified and processed for analysis."
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<http://www.eionet.europa.eu/gemet/concept/3007>,
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