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50.767]]]"}],"citation":{"authors":["Goodall, T.I.","Griffiths, R.I.","Blaud, A.","Abadie, M.","Clark, I.M.","Hirsch, P.R.","Carswell, A.M.","Misselbrook, T.H.","Sánchez-Rodríguez, A.R.","Chadwick, D.R.","Jones, D.L.","Reinsch, S."],"bibtex":"https://catalogue.ceh.ac.uk/documents/391c0294-07f1-4856-b592-428bd44055ca/citation?format=bib","day":23,"doi":"10.5285/391c0294-07f1-4856-b592-428bd44055ca","month":4,"publisher":"NERC Environmental Information Data Centre","resourceTypeGeneral":"dataset","ris":"https://catalogue.ceh.ac.uk/documents/391c0294-07f1-4856-b592-428bd44055ca/citation?format=ris","title":"Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017)","url":"https://doi.org/10.5285/391c0294-07f1-4856-b592-428bd44055ca","year":2019},"custodians":[{"organisationName":"NERC EDS Environmental Information Data 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The sites were Rothamsted Research at North Wyke in Devon  and Bangor University at Henfaes Research Station in North Wales.\n\nAt each site measurements were taken from 15 plots, organised within a randomised complete block design where 5 plots did not receive fertilizers (controls), 5 plots received food-based digestate, and 5 plots received acidified food based digestate a nitrification inhibitor. Soil samples were taken within two weeks of digestate application and shortly before winter wheat harvest.\n\nSoil chemical parameters were: soil nitrate, ammonium, dissolved organic carbon and nitrogen, amino acids and peptides, soil organic matter content as loss-on-ignition, pH, sodium, potassium, calcium, magnesium, permanganate oxdisable carbon citric acid extractable phosphorous, Olsen-P and total carbon, nitrogen and phosphorus. Soil biological measure were: microbial biomass carbon and nitrogen. \n\nSoil samples were taken by members of staff from Centre of Ecology & Hydrology (Bangor), Bangor University, School of Environment, Natural Resources & Geography Sustainable Agricultural Sciences, and Rothamsted Research North Wyke. Measurements were carried out Rothamsted Research Harpenden and the Centre of Ecology & Hydrology (Wallingford). Soil physico-chemical parameters were measured on the same soil samples and are presented in a related dataset. https://catalogue.ceh.ac.uk/id/90df9dfa-a0c8-4ead-a13d-0a0a13cda7ab\n\nData was collected for the Newton Fund project “UK-China Virtual Joint Centre for Improved Nitrogen Agronomy”. Funded by Biotechnology and Biological Sciences Research Council (BBSRC) and NERC -  Ref BB/N013468/1","distributionFormats":[{"name":"Comma-separated values (CSV)","type":"text/csv","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"funding":[{"funderName":"Biotechnology and Biological Sciences Research Council","funderIdentifier":"https://ror.org/00cwqg982","awardTitle":"Newton Fund project - UK-China Virtual Joint Centre for Improved Nitrogen Agronomy (CINAg)","awardNumber":"BB/N013468/1","awardURI":"https://gtr.ukri.org/projects?ref=BB%2FN013468%2F1","orcid":false,"ror":true}],"id":"391c0294-07f1-4856-b592-428bd44055ca","incomingCitationCount":0,"infoLinks":[{"url":"https://data-package.ceh.ac.uk/sd/391c0294-07f1-4856-b592-428bd44055ca.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"inspireThemes":[{"theme":"Environmental Monitoring Facilities","uri":"http://inspire.ec.europa.eu/theme/ef"}],"keywordsOther":[{"value":"winter wheat"},{"value":"nitrogen"},{"value":"fertiliser"},{"value":"digestate"},{"value":"operational taxonomic unit","uri":"http://vocabs.lter-europe.net/EnvThes/21637"},{"value":"diversity index","uri":"http://vocabs.lter-europe.net/EnvThes/21513"},{"value":"bacterial community composition","uri":"http://vocabs.lter-europe.net/EnvThes/20714"}],"licences":[{"value":"This resource is made available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}],"lineage":"Soil samples were taken by members of staff from the different sites. Samples were sent to Rothamsted Research Harpenden for DNA extractions and extracts were analysed for nitrogen genes (nifH, amoA, nirK, nirS, nosZ, ureC). Extracts were also sent to CEH Wallingford for analyses of operational taxonomic units (OTUs').\n\nSoil community DNA was extracted from 0.25 grams soil using the MoBio DNA PowerSoil DNA isolation kit (Mo Bio Laboratories, Inc. Carlsbad, CA), following manufacturer’s protocol. Extracted DNA was quantified by fluorometer Qubit® 2.0 dsDNA BR Assay Kit (Thermo Fisher Scientific) and quality checked by nanodrop (Thermo Fisher Scientific).\n\nQuantitative-Polymerase chain reaction (qPCR) protocols were followed as detailed in De Sosa et al. 2018.\n\nExtracted DNA was supplied from Rothamsted Research in Harpenden for amplicon sequencing at the facilities of the Molecular Ecology Group, Centre for Ecology & Hydrology, Wallingford. <50 μL volumes of DNA extract were supplied in 96-well plate format, these were subject to 16S Ribosomal ribonucleic acid (rRNA) amplicon sequencing as described in Kozich 2013 and Internal transcribed spacer (ITS) rRNA amplicon sequencing employing the Kozich 2013 strategy and Ihrmark 2012.\n\nAll results were entered into a Microsoft Excel spreadsheet and converted to .csv files for ingestion into the Environmental Information Data Centre.","metadataDate":"2026-06-29T18:13:31","notGEMINI":false,"onlineResources":[{"url":"https://data-package.ceh.ac.uk/data/391c0294-07f1-4856-b592-428bd44055ca","name":"Download the data","description":"Download a copy of this data","function":"download","type":"OTHER"},{"url":"https://data-package.ceh.ac.uk/sd/391c0294-07f1-4856-b592-428bd44055ca.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"pointsOfContact":[{"familyName":"Reinsch","givenName":"S.","organisationName":"UK Centre for Ecology & Hydrology","organisationIdentifier":"https://ror.org/00pggkr55","role":"pointOfContact","email":"enquiries@ceh.ac.uk","nameIdentifier":"https://orcid.org/0000-0003-4649-0677","address":{"deliveryPoint":"Environment Centre Wales, Deiniol Road","city":"Bangor","administrativeArea":"Gwynedd","postalCode":"LL57 2UW","country":"United Kingdom"},"fullName":"Reinsch, 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Highlander, S. K., & Schloss, P.D. (2013). Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform. Applied and Environmental Microbiology, 79(17), 5112–5120.","url":"https://doi.org/10.1128/aem.01043-13","function":"relatedArticle"},{"description":"de Sosa, L.L., Glanville, H.C., Marshall, M.R., Schnepf, A., Cooper, D.M., Hill, P.W., … Jones, D.L. (2018). Stoichiometric constraints on the microbial processing of carbon with soil depth along a riparian hillslope. Biology and Fertility of Soils, 54(8), 949–963.","url":"https://doi.org/10.1007/s00374-018-1317-2","function":"relatedArticle"}],"temporalExtents":[{"begin":"2017-04-01","end":"2017-10-31"}],"title":"Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017)","topicCategories":[{"value":"farming","uri":"http://inspire.ec.europa.eu/metadata-codelist/TopicCategory/farming"}],"type":"dataset","uri":"https://catalogue.ceh.ac.uk/id/391c0294-07f1-4856-b592-428bd44055ca","useConstraints":[{"value":"This resource is made available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"},{"value":"© Rothamsted Research","code":"copyright"},{"value":"© UK Centre for Ecology & Hydrology","code":"copyright"},{"value":"© Bangor University","code":"copyright"}]}