PREFIX rdf: PREFIX rdfs: PREFIX skos: PREFIX owl: PREFIX xsd: PREFIX dcterms: PREFIX dcat: PREFIX dcmitype: PREFIX foaf: PREFIX geo: PREFIX vcard: PREFIX prov: PREFIX odrs: PREFIX sosa: PREFIX sdo: PREFIX ef: PREFIX doo: PREFIX adms: PREFIX : :3ca94e73-57dc-4fcd-afe2-82a4e491970a dcterms:title "River phytoplankton growth responses to organic nutrients with associated nutrient data from 25 rivers in GB" ; dcterms:identifier "https://catalogue.ceh.ac.uk/id/3ca94e73-57dc-4fcd-afe2-82a4e491970a","https://doi.org/10.5285/3ca94e73-57dc-4fcd-afe2-82a4e491970a" ; dcterms:bibliographicCitation "Mackay, E.B., Feuchtmayr, H., Thackeray, S.J., Callaghan, N., Marshall, M., Rhodes, G., Yates, C., Johnes, P.J., Maberly, S.C. (2025). River phytoplankton growth responses to organic nutrients with associated nutrient data from 25 rivers in GB. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/3ca94e73-57dc-4fcd-afe2-82a4e491970a" ; dcterms:description "The data are from bioassay experiments from 25 river sites across Great Britain to investigate the growth response of river phytoplankton to different inorganic and organic nutrient additions and compare that to in situ nutrient concentrations. The data includes calculated growth responses to inorganic and organic nutrient additions, based on experimental incubations and background nutrient data collected from sites at the same time as water for the experimental incubations." ; dcterms:provenance [ a dcterms:ProvenanceStatement ; rdfs:label "Raw water samples were collected at each site on a single sampling occasion and kept at 4°C in the dark and transported to the laboratory within 48 hours. They were acclimatised in a constant temperature room at 20°C. Water was filtered through a 100 µm mesh to remove any zooplankton or large detritus present, 35 mL of sample was transferred to 50 mL boiling tubes. 12 different nutrient treatments were applied at approximately Redfield ratio [N: 90 µmol L-1 (1.261 mg N L-1) and P: 6 µmol L-1 (0.186 mg P L-1)] in triplicate to quantify growth responses to both organic N and P. These included: 1) a control with no addition of nutrients, 2) inorganic N only, 3) inorganic P only and 4) inorganic N and P, 5-8) four organic N additions and 9- 12) four organic P additions. Tubes were stoppered with foam bungs enabling gas exchange and incubated for 14 days at 20°C under a photon irradiance of 80 – 120 µmol m-2 s-1 (photosynthetically available radiation, Macam Q102) and an 18 h light, 6 h dark cycle. After the incubation period, tube contents including biofilm were resuspended by scraping and vortex mixing before being filtered onto a Whatman GF/C glass fibre filter (nominal pore size 1.2 µm) and frozen. Filters were later defrosted, and chlorophyll a extracted in hot methanol and measured spectrophotometrically. Growth response was determined as a change in the concentration of chlorophyll a in the treatment relative to the relevant control calculated as a natural log response ratio. For the water chemistry data (measured in mg/L), a Skalar++ multi-channel continuous flow autoanalyzer (Skalar Analytical B.V., The Netherlands) was used to carry out inorganic nutrient analysis, including total and total dissolved N and P (TN or TP, TDN or TDP) following persulphate digestion of unfiltered and filtered (pre-leached 0.45 µm cellulose nitrate) samples respectively, and dissolved inorganic N (DIN), including total ammoniacal nitrogen (NH3 and NH4), or soluble reactive P (SRP) on filtered samples. Dissolved organic nitrogen (DON) and dissolved organic phosphorus (DOP), were determined by difference between TDN and DIN or TDP and SRP, respectively. Data from these analyses were checked and entered into spreadsheets for later analysis." ] ; dcterms:spatial [ a dcterms:Location ; dcat:bbox "POLYGON((-8.648 49.864, -8.648 60.861, 1.768 60.861, 1.768 49.864, -8.648 49.864))"^^geo:wktLiteral ; ] ; dcterms:temporal [ a dcterms:PeriodOfTime ; dcat:startDate "2017-09-05"^^xsd:date ; dcat:endDate "2017-10-26"^^xsd:date ; ] ; dcat:contactPoint :3ca94e73-57dc-4fcd-afe2-82a4e491970a_c0 ; dcterms:publisher ; dcterms:subject "Great Britain", , "Bioassay", "Organic nutrients", "River phytoplankton", "Nutrient limitation", "DOMAINE", , ; prov:wasGeneratedBy :3ca94e73-57dc-4fcd-afe2-82a4e491970a_fund0 ; a dcat:Dataset; dcterms:type dcmitype:Dataset ; dcterms:available "2025-03-27"^^xsd:date ; dcat:landingPage , ; dcterms:license ; dcterms:accessRights ; dcterms:theme ; dcat:distribution [ dcat:accessURL ; dcterms:license ; dcterms:accessRights ; dcterms:format [ a dcterms:IMT ; rdf:value "Comma-separated values (CSV)" ; rdfs:label "Comma-separated values (CSV)" ] ; ]; dcterms:creator , :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a1, :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a2, :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a3, :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a4, :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a5, :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a6, :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a7, :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a8 ; dcterms:language . :3ca94e73-57dc-4fcd-afe2-82a4e491970a_c0 a foaf:Person ; foaf:name "Mackay, E.B." ; foaf:familyName "Mackay" ; foaf:givenName "E.B." ; vcard:hasEmail "enquiries@ceh.ac.uk" ; foaf:member ; . a foaf:Organization ; foaf:name "NERC EDS Environmental Information Data Centre" ; vcard:hasEmail "info@eidc.ac.uk" ; foaf:member ; . a foaf:Person ; foaf:name "Mackay, E.B." ; foaf:familyName "Mackay" ; foaf:givenName "E.B." ; vcard:hasEmail "enquiries@ceh.ac.uk" ; foaf:member ; . :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a1 a foaf:Person ; foaf:name "Feuchtmayr, H." ; foaf:familyName "Feuchtmayr" ; foaf:givenName "H." ; vcard:hasEmail "enquiries@ceh.ac.uk" ; foaf:member ; . :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a2 a foaf:Person ; foaf:name "Thackeray, S.J." ; foaf:familyName "Thackeray" ; foaf:givenName "S.J." ; vcard:hasEmail "enquiries@ceh.ac.uk" ; foaf:member ; . :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a3 a foaf:Person ; foaf:name "Callaghan, N." ; foaf:familyName "Callaghan" ; foaf:givenName "N." ; vcard:hasEmail "enquiries@ceh.ac.uk" ; foaf:member ; . :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a4 a foaf:Person ; foaf:name "Marshall, M." ; foaf:familyName "Marshall" ; foaf:givenName "M." ; vcard:hasEmail "m.marshall@bangor.ac.uk" ; foaf:member ; . :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a5 a foaf:Person ; foaf:name "Rhodes, G." ; foaf:familyName "Rhodes" ; foaf:givenName "G." ; vcard:hasEmail "enquiries@ceh.ac.uk" ; foaf:member ; . :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a6 a foaf:Person ; foaf:name "Yates, C." ; foaf:familyName "Yates" ; foaf:givenName "C." ; vcard:hasEmail "Christopher.Yates@atkinsrealis.com" ; foaf:member [foaf:name "Atkins Realis"] ; . :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a7 a foaf:Person ; foaf:name "Johnes, P.J." ; foaf:familyName "Johnes" ; foaf:givenName "P.J." ; vcard:hasEmail "penny.johnes@bristol.ac.uk" ; foaf:member ; . :3ca94e73-57dc-4fcd-afe2-82a4e491970a_a8 a foaf:Person ; foaf:name "Maberly, S.C." ; foaf:familyName "Maberly" ; foaf:givenName "S.C." ; vcard:hasEmail "enquiries@ceh.ac.uk" ; foaf:member ; . a skos:Concept; skos:prefLabel "Water quality"; rdfs:label "Water quality" . a skos:Concept; skos:prefLabel "nutrient"; rdfs:label "nutrient" . a skos:Concept; skos:prefLabel "phytoplankton"; rdfs:label "phytoplankton" . a foaf:Organization ; foaf:name "UK Centre for Ecology & Hydrology" . a foaf:Organization ; foaf:name "Bangor University" . a foaf:Organization ; foaf:name "Bristol University" . :3ca94e73-57dc-4fcd-afe2-82a4e491970a_fund0 a prov:Activity ; .