PREFIX rdf: PREFIX rdfs: PREFIX skos: PREFIX owl: PREFIX xsd: PREFIX dcterms: PREFIX dcat: PREFIX dcmitype: PREFIX foaf: PREFIX geo: PREFIX prov: PREFIX odrs: PREFIX sosa: PREFIX sdo: PREFIX ef: PREFIX doo: PREFIX adms: PREFIX frapo: PREFIX pro: PREFIX scoro: PREFIX : :3ca94e73-57dc-4fcd-afe2-82a4e491970a dcterms:title "River phytoplankton growth responses to organic nutrients with associated nutrient data from 25 rivers in GB" ; dcterms:identifier ; adms:identifier ; dcterms:bibliographicCitation "Mackay, E.B., Feuchtmayr, H., Thackeray, S.J., Callaghan, N., Marshall, M., Rhodes, G., Yates, C., Johnes, P.J., Maberly, S.C. (2025). River phytoplankton growth responses to organic nutrients with associated nutrient data from 25 rivers in GB. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/3ca94e73-57dc-4fcd-afe2-82a4e491970a" ; dcterms:description "The data are from bioassay experiments from 25 river sites across Great Britain to investigate the growth response of river phytoplankton to different inorganic and organic nutrient additions and compare that to in situ nutrient concentrations. The data includes calculated growth responses to inorganic and organic nutrient additions, based on experimental incubations and background nutrient data collected from sites at the same time as water for the experimental incubations." ; dcterms:provenance [ a dcterms:ProvenanceStatement ; rdfs:label "Raw water samples were collected at each site on a single sampling occasion and kept at 4°C in the dark and transported to the laboratory within 48 hours. They were acclimatised in a constant temperature room at 20°C. Water was filtered through a 100 µm mesh to remove any zooplankton or large detritus present, 35 mL of sample was transferred to 50 mL boiling tubes. 12 different nutrient treatments were applied at approximately Redfield ratio [N: 90 µmol L-1 (1.261 mg N L-1) and P: 6 µmol L-1 (0.186 mg P L-1)] in triplicate to quantify growth responses to both organic N and P. These included: 1) a control with no addition of nutrients, 2) inorganic N only, 3) inorganic P only and 4) inorganic N and P, 5-8) four organic N additions and 9- 12) four organic P additions. Tubes were stoppered with foam bungs enabling gas exchange and incubated for 14 days at 20°C under a photon irradiance of 80 – 120 µmol m-2 s-1 (photosynthetically available radiation, Macam Q102) and an 18 h light, 6 h dark cycle. After the incubation period, tube contents including biofilm were resuspended by scraping and vortex mixing before being filtered onto a Whatman GF/C glass fibre filter (nominal pore size 1.2 µm) and frozen. Filters were later defrosted, and chlorophyll a extracted in hot methanol and measured spectrophotometrically. Growth response was determined as a change in the concentration of chlorophyll a in the treatment relative to the relevant control calculated as a natural log response ratio. For the water chemistry data (measured in mg/L), a Skalar++ multi-channel continuous flow autoanalyzer (Skalar Analytical B.V., The Netherlands) was used to carry out inorganic nutrient analysis, including total and total dissolved N and P (TN or TP, TDN or TDP) following persulphate digestion of unfiltered and filtered (pre-leached 0.45 µm cellulose nitrate) samples respectively, and dissolved inorganic N (DIN), including total ammoniacal nitrogen (NH3 and NH4), or soluble reactive P (SRP) on filtered samples. Dissolved organic nitrogen (DON) and dissolved organic phosphorus (DOP), were determined by difference between TDN and DIN or TDP and SRP, respectively. Data from these analyses were checked and entered into spreadsheets for later analysis." ] ; dcterms:spatial [ a dcterms:Location ; dcat:bbox "POLYGON((-8.648 49.864, -8.648 60.861, 1.768 60.861, 1.768 49.864, -8.648 49.864))"^^geo:wktLiteral ; ] ; dcterms:temporal [ a dcterms:PeriodOfTime ; dcat:startDate "2017-09-05"^^xsd:date ; dcat:endDate "2017-10-26"^^xsd:date ; ] ; dcat:contactPoint :person_1ed45344c6789c24 ; dcterms:publisher ; dcterms:subject "Great Britain", , "Bioassay", "Organic nutrients", "River phytoplankton", "Nutrient limitation", "DOMAINE", , ; prov:wasGeneratedBy :grant_4817c127b503c2aa ; a dcat:Dataset; dcterms:type dcmitype:Dataset ; dcterms:available "2025-03-27"^^xsd:date ; dcat:landingPage , ; dcterms:license ; dcterms:accessRights ; dcterms:theme ; dcat:distribution [ dcat:accessURL ; dcterms:license ; dcterms:accessRights ; dcterms:format ; ]; dcterms:creator ,:person_8266099aec48ad12,:person_8144b3e3f8f9d87e,:person_e635be0b16c6bd39,:person_42154ec5cb1e8dbf,:person_10cf6c75451149a6,:person_a7490fa20f4174a0,:person_d0012c4e505204c1,:person_77be7afc35138d7f ; dcterms:language . :person_1ed45344c6789c24 a foaf:Person ; foaf:name "Mackay, E.B." ; foaf:familyName "Mackay" ; foaf:givenName "E.B." ; foaf:member ; . a foaf:Organization ; . a foaf:Person ; foaf:member ; . pro:holdsRoleInTime :role_bfcb5fb4345f38c2 . :role_bfcb5fb4345f38c2 a pro:RoleInTime ; pro:withRole pro:author ; pro:relatesToEntity :3ca94e73-57dc-4fcd-afe2-82a4e491970a . :person_8266099aec48ad12 a foaf:Person ; foaf:name "Feuchtmayr, H." ; foaf:familyName "Feuchtmayr" ; foaf:givenName "H." ; foaf:member ; . :person_8266099aec48ad12 pro:holdsRoleInTime :role_662585c8c2a2be84 . :role_662585c8c2a2be84 a pro:RoleInTime ; pro:withRole pro:author ; pro:relatesToEntity :3ca94e73-57dc-4fcd-afe2-82a4e491970a . :person_8144b3e3f8f9d87e a foaf:Person ; foaf:name "Thackeray, S.J." ; foaf:familyName "Thackeray" ; foaf:givenName "S.J." ; foaf:member ; . :person_8144b3e3f8f9d87e pro:holdsRoleInTime :role_4af1d99ebbdc94dc . :role_4af1d99ebbdc94dc a pro:RoleInTime ; pro:withRole pro:author ; pro:relatesToEntity :3ca94e73-57dc-4fcd-afe2-82a4e491970a . :person_e635be0b16c6bd39 a foaf:Person ; foaf:name "Callaghan, N." ; foaf:familyName "Callaghan" ; foaf:givenName "N." ; foaf:member ; . :person_e635be0b16c6bd39 pro:holdsRoleInTime :role_c2c1f14f18d97637 . :role_c2c1f14f18d97637 a pro:RoleInTime ; pro:withRole pro:author ; pro:relatesToEntity :3ca94e73-57dc-4fcd-afe2-82a4e491970a . :person_42154ec5cb1e8dbf a foaf:Person ; foaf:name "Marshall, M." ; foaf:familyName "Marshall" ; foaf:givenName "M." ; foaf:member ; . :person_42154ec5cb1e8dbf pro:holdsRoleInTime :role_a53fd6c0962a722d . :role_a53fd6c0962a722d a pro:RoleInTime ; pro:withRole pro:author ; pro:relatesToEntity :3ca94e73-57dc-4fcd-afe2-82a4e491970a . :person_10cf6c75451149a6 a foaf:Person ; foaf:name "Rhodes, G." ; foaf:familyName "Rhodes" ; foaf:givenName "G." ; foaf:member ; . :person_10cf6c75451149a6 pro:holdsRoleInTime :role_b21a500b45771320 . :role_b21a500b45771320 a pro:RoleInTime ; pro:withRole pro:author ; pro:relatesToEntity :3ca94e73-57dc-4fcd-afe2-82a4e491970a . :person_a7490fa20f4174a0 a foaf:Person ; foaf:name "Yates, C." ; foaf:familyName "Yates" ; foaf:givenName "C." ; foaf:member :organisation_9676b1b1fb6d1320 ; . :organisation_9676b1b1fb6d1320 a foaf:Organization ; foaf:name "Atkins Realis" . :person_a7490fa20f4174a0 pro:holdsRoleInTime :role_bbc39ba0d60cf4ca . :role_bbc39ba0d60cf4ca a pro:RoleInTime ; pro:withRole pro:author ; pro:relatesToEntity :3ca94e73-57dc-4fcd-afe2-82a4e491970a . :person_d0012c4e505204c1 a foaf:Person ; foaf:name "Johnes, P.J." ; foaf:familyName "Johnes" ; foaf:givenName "P.J." ; foaf:member ; . :person_d0012c4e505204c1 pro:holdsRoleInTime :role_b2399a5f2f94bd76 . :role_b2399a5f2f94bd76 a pro:RoleInTime ; pro:withRole pro:author ; pro:relatesToEntity :3ca94e73-57dc-4fcd-afe2-82a4e491970a . :person_77be7afc35138d7f a foaf:Person ; foaf:name "Maberly, S.C." ; foaf:familyName "Maberly" ; foaf:givenName "S.C." ; foaf:member ; . :person_77be7afc35138d7f pro:holdsRoleInTime :role_77be43ff1e4b6c9f . :role_77be43ff1e4b6c9f a pro:RoleInTime ; pro:withRole pro:author ; pro:relatesToEntity :3ca94e73-57dc-4fcd-afe2-82a4e491970a . a skos:Concept . a skos:Concept . a skos:Concept . a foaf:Organization . a foaf:Organization . a foaf:Organization . a dcterms:IMT . :grant_4817c127b503c2aa a frapo:Grant, prov:Activity ; frapo:hasGrantNumber "NE/K010603/1" ; frapo:funds :3ca94e73-57dc-4fcd-afe2-82a4e491970a ; . a frapo:FundingAgency ; frapo:awards :grant_4817c127b503c2aa .