Growth of poplar seedlings grown in chambers following variations in photoperiod and temperature
type: Dataset
citation: Hellmann, E., Friend, A.D. (2025). Growth of poplar seedlings grown in chambers following variations in photoperiod and temperature. NERC EDS Environmental Information Data Centre. (Dataset). https://doi.org/10.5285/46c23be6-63ba-400d-859b-050dcb207898
description: This dataset contains information on how variations on temperature and photoperiod affects poplar seedling growth in Eco chambers. Plants were propagated on sterile media (1/2MS, 0.7% Agar, 1mg/L IAA) by cutting the shoot tip and 3-4 leaves and placing them in a weck jar on new medium. Once the explants rooted after incubation at 200 mmol light, 21C day/18C night, long day conditions (16:8) and ambient humidity, they were transplanted onto soil (6:2:1 Peat-free: sand-vermiculite, fertilizer: 4 g/L Osmocote exact standard 15-9-12) and grown first under protective covers and then open in 24C, long day conditions (LD), 65% humidity, 350mmol light (experiments R1-3) or transferred into the Eco chamber (temperature as indicated in experiment, 80-98% humidity, 350mmol LED light, long day conditions). After 3-4 weeks, plants of R1-R3 were transferred in the conditions described in the experiment (R1: 24C SD vs 15C LD; R2: 15C SD vs 15C LD; R3: 15C LD vs 24C LD) and grown for a month before moving them into 5C low light conditions which resulted in senescence and leaf drop. Eco chamber plants were grown in the Eco chamber for about four weeks and after not showing senescence, harvested directly after the experiment without cold incubation. All plants were measured in height and diameter (6cm above soil), leaf number and area regularly and gas exchanged was measured with a TARGAS-1 instrument (Hansatech) at 500mmol light intensity. After the experiment, cross-sections were taken and embedded in resin for further microscopic analysis (data not ready and not included).
is accessible for free: true
date published: 2025-04-17
keywords: day-length
keywords: growth
keywords: Poplar
keywords: photosynthesis
keywords: temperature
creator: Hellmann, E.
creator: Friend, A.D.
temporal coverage: 2023-01-01/2023-12-31
license: Open Government Licence v3
distribution: distribution0
publisher: NERC EDS Environmental Information Data Centre
provider: NERC EDS Environmental Information Data Centre
included in data catalog: Environmental Information Data Centre

photosynthesis
type: DefinedTerm
in defined term set: https://www.eionet.europa.eu/gemet/

temperature
type: DefinedTerm
in defined term set: https://www.eionet.europa.eu/gemet/

Environmental Information Data Centre
type: DataCatalog
alternate name: EIDC

NERC EDS Environmental Information Data Centre
type: Organization

Open Government Licence v3
type: CreativeWork
alternate name: OGL-UK-3.0

Hellmann, E.
type: Person
family name: Hellmann
given name: E.
affiliation: Department of Geography, Cambridge, The Sainsbury Laboratory, Cambrigde

Friend, A.D.
type: Person
family name: Friend
given name: A. D.
affiliation: Department of Geography, Cambridge

distribution0
type: DataDownload
encoding format: application/zip