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The bacteria were originally isolated from a nature reserve in Minnesota USA. Daily samples of spot cultures grown in adjacent pairs on solid ISP2 medium were taken from day 2 to 6 in triplicate. Metabolites were obtained by cold methanol extraction, and liquid chromatography-mass spectrometry data was collected in both positive and negative ionisation modes. This dataset contains raw LC-MS (liquid chromatography mass spectrometry) data converted to the open-source .mzML format, of each sample run in both positive and negative ionisation modes. This includes, mass-to-charge measurements, elution time, and ion count intensity. These data were collected to investigate how the metabolism of the interacting strains changes in response to neighbouring bacterial cultures.\nThis work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458).\n","distributionFormats":[{"name":".mzML","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"funding":[{"funderName":"Natural Environment Research Council","funderIdentifier":"https://ror.org/02b5d8509","awardNumber":"NE/T010959/1","orcid":false,"ror":true},{"funderName":"National Science Foundation","awardNumber":"1935458","awardURI":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=1935458","orcid":false,"ror":false}],"hasOnlineServiceAgreement":false,"id":"475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e","incomingCitationCount":0,"infoLinks":[{"url":"https://data-package.ceh.ac.uk/sd/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"keywordsOther":[{"value":"bacteria","uri":"http://vocabs.lter-europe.net/EnvThes/20662"},{"value":"microbiology","uri":"http://vocabs.lter-europe.net/EnvThes/21641"},{"value":"co-culture"},{"value":"microbiome"},{"value":"streptomyces"},{"value":"mass spectrometry"},{"value":"actinomycetes"},{"value":"metabolomics"},{"value":"untargeted"},{"value":"mzml"}],"keywordsTheme":[{"value":"Biodiversity","uri":"http://onto.nerc.ac.uk/CEHMD/topic/2"}],"licences":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}],"lineage":"The 4 Streptomyces strains were previously isolated from Minnesota soil as described in Schlatter, D.C. et al (2013), co-culturing, metabolite extraction and LC-MS experiments were performed in the Manchester Institute of Biotechnology, University of Manchester, UK. Data was collected on a Q-Exactive Mass Spectrometer (ThermoFisher). Outputs were converted to mzML format by the ProteoWizard MS converter. Pooled quality control samples were analysed at every 6th injection to assess for analytical drift over time. The sample injection sequence was randomised.","metadataDate":"2026-06-29T18:12:26","notGEMINI":false,"onlineResources":[{"url":"https://catalogue.ceh.ac.uk/datastore/eidchub/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e","name":"Download the data","description":"Download a copy of this data","function":"fileAccess","type":"OTHER"},{"url":"https://data-package.ceh.ac.uk/sd/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"pointsOfContact":[{"familyName":"Connolly","givenName":"J. 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