PREFIX rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#>
PREFIX rdfs: <http://www.w3.org/2000/01/rdf-schema#>
PREFIX skos: <http://www.w3.org/2004/02/skos/core#>
PREFIX owl: <http://www.w3.org/2002/07/owl#>
PREFIX xsd: <http://www.w3.org/2001/XMLSchema#>
PREFIX dcterms: <http://purl.org/dc/terms/>
PREFIX dcat: <http://www.w3.org/ns/dcat#>
PREFIX dcmitype: <http://purl.org/dc/dcmitype/>
PREFIX foaf: <http://xmlns.com/foaf/0.1/>
PREFIX geo: <http://www.opengis.net/ont/geosparql#>
PREFIX prov: <http://www.w3.org/ns/prov#>
PREFIX odrs: <http://schema.theodi.org/odrs#>
PREFIX sosa: <http://www.w3.org/ns/sosa/>
PREFIX sdo: <https://schema.org/>
PREFIX ef: <http://www.w3.org/2015/03/inspire/ef/>
PREFIX doo: <https://digital.ceh.ac.uk/ontology/doo/>
PREFIX adms: <http://www.w3.org/ns/adms#>
PREFIX frapo: <http://purl.org/cerif/frapo/>
PREFIX pro: <http://purl.org/spar/pro/>
PREFIX scoro: <http://purl.org/spar/scoro/>
PREFIX : <https://catalogue.ceh.ac.uk/id/>
:475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
dcterms:title "Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA" ;
dcterms:identifier <https://catalogue.ceh.ac.uk/id/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e> ;
adms:identifier <https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e> ;
dcterms:bibliographicCitation "Connolly, J.A., Del Carratore, F., Schmidt, K., Bisesi, A., Martinson, J.N.V., Chua, J., Kuhs, M., Boneza, M., Heinsch, S., Kinkel, L., Smanski, M., Harcombe, W.R., Breitling, R., Takano, E. (2025). Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e" ;
dcterms:description "This dataset contains untargeted metabolomic profiles of paired cultures of 4 interacting Streptomyces bacteria. The bacteria were originally isolated from a nature reserve in Minnesota USA. Daily samples of spot cultures grown in adjacent pairs on solid ISP2 medium were taken from day 2 to 6 in triplicate. Metabolites were obtained by cold methanol extraction, and liquid chromatography-mass spectrometry data was collected in both positive and negative ionisation modes. This dataset contains raw LC-MS (liquid chromatography mass spectrometry) data converted to the open-source .mzML format, of each sample run in both positive and negative ionisation modes. This includes, mass-to-charge measurements, elution time, and ion count intensity. These data were collected to investigate how the metabolism of the interacting strains changes in response to neighbouring bacterial cultures. This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458)." ;
dcterms:provenance [
a dcterms:ProvenanceStatement ;
rdfs:label "The 4 Streptomyces strains were previously isolated from Minnesota soil as described in Schlatter, D.C. et al (2013), co-culturing, metabolite extraction and LC-MS experiments were performed in the Manchester Institute of Biotechnology, University of Manchester, UK. Data was collected on a Q-Exactive Mass Spectrometer (ThermoFisher). Outputs were converted to mzML format by the ProteoWizard MS converter. Pooled quality control samples were analysed at every 6th injection to assess for analytical drift over time. The sample injection sequence was randomised."
] ;
dcat:contactPoint :person_18a783b473ab56c0 ;
dcterms:publisher <https://ror.org/04xw4m193> ;
dcterms:relation <https://catalogue.ceh.ac.uk/id/4921f306-5efa-4bf7-a15e-a157c2665889> ;
dcterms:subject
<http://onto.nerc.ac.uk/CEHMD/topic/2>,
<http://vocabs.lter-europe.net/EnvThes/20662>,
<http://vocabs.lter-europe.net/EnvThes/21641>,
"co-culture",
"microbiome",
"streptomyces",
"mass spectrometry",
"actinomycetes",
"metabolomics",
"untargeted",
"mzml" ;
prov:wasGeneratedBy :grant_ccd9cc12206d6ff0,:grant_da512f785731e9cf ;
a dcat:Dataset;
dcterms:type dcmitype:Dataset ;
dcterms:available "2025-02-12"^^xsd:date ;
dcat:landingPage <https://catalogue.ceh.ac.uk/id/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e>, <https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e> ;
dcterms:license <https://spdx.org/licenses/OGL-UK-3.0.ttl>
;
dcterms:accessRights <http://vocab.nerc.ac.uk/collection/N07/current/UNRS/> ;
dcterms:theme
<http://onto.nerc.ac.uk/CEHMD/topic/2> ;
dcterms:creator <https://orcid.org/0000-0003-3643-5578>,<https://orcid.org/0000-0003-1647-7818>,:person_f326ce3672be8a04,:person_4c20729fbe68c16e,:person_84740e16e0588f99,:person_cbc9db15512a1f5f,:person_595fb93330e67ed5,:person_c6781ccb6fcd27b8,:person_50edd2f34a34ebf1,<https://orcid.org/0000-0002-5331-5589>,:person_a11b4bb4bd5a0e57,:person_0d4da2d9cdee82de,<https://orcid.org/0000-0001-7173-0922>,<https://orcid.org/0000-0002-6791-3256> ;
dcterms:language <http://id.loc.gov/vocabulary/iso639-1/en> .
:person_18a783b473ab56c0 a foaf:Person ;
foaf:name "Connolly, J.A." ;
foaf:familyName "Connolly" ;
foaf:givenName "J. A." ;
foaf:member :organisation_47ce0218bbead4aa ;
.
:organisation_47ce0218bbead4aa a foaf:Organization ;
foaf:name "University of Manchester" .
<https://ror.org/04xw4m193> a foaf:Organization ;
.
<https://orcid.org/0000-0003-3643-5578> a foaf:Person ;
foaf:member <https://ror.org/027m9bs27> ;
.
<https://orcid.org/0000-0003-3643-5578> pro:holdsRoleInTime :role_bc8ab5f3a8457c6f .
:role_bc8ab5f3a8457c6f a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
<https://orcid.org/0000-0003-1647-7818> a foaf:Person ;
foaf:member <https://ror.org/027m9bs27> ;
.
<https://orcid.org/0000-0003-1647-7818> pro:holdsRoleInTime :role_7c0c4edb4a47603c .
:role_7c0c4edb4a47603c a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
:person_f326ce3672be8a04 a foaf:Person ;
foaf:name "Schmidt, K." ;
foaf:familyName "Schmidt" ;
foaf:givenName "K." ;
foaf:member <https://ror.org/027m9bs27> ;
.
:person_f326ce3672be8a04 pro:holdsRoleInTime :role_613e4a854d153cd8 .
:role_613e4a854d153cd8 a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
:person_4c20729fbe68c16e a foaf:Person ;
foaf:name "Bisesi, A." ;
foaf:familyName "Bisesi" ;
foaf:givenName "A." ;
foaf:member <https://ror.org/017zqws13> ;
.
:person_4c20729fbe68c16e pro:holdsRoleInTime :role_bdfd2da692ecb765 .
:role_bdfd2da692ecb765 a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
:person_84740e16e0588f99 a foaf:Person ;
foaf:name "Martinson, J.N.V." ;
foaf:familyName "Martinson" ;
foaf:givenName "J. N. V." ;
foaf:member <https://ror.org/017zqws13> ;
.
:person_84740e16e0588f99 pro:holdsRoleInTime :role_28181fb7a725c510 .
:role_28181fb7a725c510 a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
:person_cbc9db15512a1f5f a foaf:Person ;
foaf:name "Chua, J." ;
foaf:familyName "Chua" ;
foaf:givenName "J." ;
foaf:member <https://ror.org/027m9bs27> ;
.
:person_cbc9db15512a1f5f pro:holdsRoleInTime :role_bfcdde7435a3da78 .
:role_bfcdde7435a3da78 a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
:person_595fb93330e67ed5 a foaf:Person ;
foaf:name "Kuhs, M." ;
foaf:familyName "Kuhs" ;
foaf:givenName "M." ;
foaf:member <https://ror.org/017zqws13> ;
.
:person_595fb93330e67ed5 pro:holdsRoleInTime :role_f9fae594aa6997d8 .
:role_f9fae594aa6997d8 a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
:person_c6781ccb6fcd27b8 a foaf:Person ;
foaf:name "Boneza, M." ;
foaf:familyName "Boneza" ;
foaf:givenName "M." ;
foaf:member <https://ror.org/017zqws13> ;
.
:person_c6781ccb6fcd27b8 pro:holdsRoleInTime :role_b12598ab4d9b3be1 .
:role_b12598ab4d9b3be1 a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
:person_50edd2f34a34ebf1 a foaf:Person ;
foaf:name "Heinsch, S." ;
foaf:familyName "Heinsch" ;
foaf:givenName "S." ;
foaf:member <https://ror.org/017zqws13> ;
.
:person_50edd2f34a34ebf1 pro:holdsRoleInTime :role_193fb455e96b60db .
:role_193fb455e96b60db a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
<https://orcid.org/0000-0002-5331-5589> a foaf:Person ;
foaf:member <https://ror.org/017zqws13> ;
.
<https://orcid.org/0000-0002-5331-5589> pro:holdsRoleInTime :role_0c390b72a3348633 .
:role_0c390b72a3348633 a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
:person_a11b4bb4bd5a0e57 a foaf:Person ;
foaf:name "Smanski, M." ;
foaf:familyName "Smanski" ;
foaf:givenName "M." ;
foaf:member <https://ror.org/017zqws13> ;
.
:person_a11b4bb4bd5a0e57 pro:holdsRoleInTime :role_f92ed690166b722b .
:role_f92ed690166b722b a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
:person_0d4da2d9cdee82de a foaf:Person ;
foaf:name "Harcombe, W.R." ;
foaf:familyName "Harcombe" ;
foaf:givenName "W. R." ;
foaf:member <https://ror.org/017zqws13> ;
.
:person_0d4da2d9cdee82de pro:holdsRoleInTime :role_30ca64e2cbdf8559 .
:role_30ca64e2cbdf8559 a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
<https://orcid.org/0000-0001-7173-0922> a foaf:Person ;
foaf:member <https://ror.org/027m9bs27> ;
.
<https://orcid.org/0000-0001-7173-0922> pro:holdsRoleInTime :role_75702cb13c3893a5 .
:role_75702cb13c3893a5 a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
<https://orcid.org/0000-0002-6791-3256> a foaf:Person ;
foaf:member <https://ror.org/027m9bs27> ;
.
<https://orcid.org/0000-0002-6791-3256> pro:holdsRoleInTime :role_8748432e4206863b .
:role_8748432e4206863b a pro:RoleInTime ;
pro:withRole pro:author ;
pro:relatesToEntity :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e .
<http://onto.nerc.ac.uk/CEHMD/topic/2> a skos:Concept .<http://vocabs.lter-europe.net/EnvThes/20662> a skos:Concept .<http://vocabs.lter-europe.net/EnvThes/21641> a skos:Concept .
<https://ror.org/027m9bs27> a foaf:Organization .
<https://ror.org/017zqws13> a foaf:Organization .
:grant_ccd9cc12206d6ff0 a frapo:Grant, prov:Activity ;
frapo:hasGrantNumber "NE/T010959/1" ;
frapo:funds :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e ;
.
<https://ror.org/02b5d8509> a frapo:FundingAgency ;
frapo:awards :grant_ccd9cc12206d6ff0 .
:grant_da512f785731e9cf a frapo:Grant, prov:Activity ;
frapo:hasGrantNumber "1935458" ;
owl:sameAs <https://www.nsf.gov/awardsearch/show-award?AWD_ID=1935458> ;
frapo:funds :475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e ;
.