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All 323 genotypes were planted in two different Locations: 1) University of Agriculture, Faisalabad (31.4396° N, 73.0768° E), and 2) National Institute of Genomics and Advanced Biotechnology (NIGAB), Islamabad (33.6736° N, 73.1240° E). The experiments were laid down for two years; Yr1) Rabi-2021-22 and Yr2) Rabi-2022-23. Alpha lattice was used as an experimental design with 4 replications at location 1 and 3 replications at location 2. Treatments were applied as 100% of the recommended N dose at a rate of 120 kg ha-1 (N100) and 50% of the recommended dose at a rate of 60 kg ha-1(N50). Urea was used as N source and applied in three splits as basal dose, at stem elongation stage and at the heading stage. Standard irrigation and crop management practices were followed for all environments. The following parameters were measured: days to flowering, flag leaf area (cm2), peduncle length (cm), spike length (cm), plant height (cm), straw yield (g), grain yield (g) and thousand grain weight (g). The work was supported by the Natural Environment Research Council (Grant NE/S009019/1).","distributionFormats":[{"name":"Comma-separated values (CSV)","type":"text/csv","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"funding":[{"funderName":"Natural Environment Research Council","funderIdentifier":"https://ror.org/02b5d8509","awardTitle":"GCRF South Asian Nitrogen Hub","awardNumber":"NE/S009019/1","awardURI":"https://sanh.inms.international/","orcid":false,"ror":true}],"hasOnlineServiceAgreement":true,"id":"7aa32225-f277-4ff6-bc73-7fe6123748cc","incomingCitationCount":0,"infoLinks":[{"url":"https://data-package.ceh.ac.uk/sd/7aa32225-f277-4ff6-bc73-7fe6123748cc.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"keywordsOther":[{"value":"nitrogenous fertiliser","uri":"http://www.eionet.europa.eu/gemet/concept/11209"},{"value":"nitrogen use efficiency"},{"value":"Pakistan"},{"value":"wheat"}],"keywordsTheme":[{"value":"Agriculture","uri":"http://onto.nerc.ac.uk/CEHMD/topic/1"}],"licences":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}],"lineage":"The following parameters were measured: days to flowering, flag leaf area (cm2), peduncle length (cm), spike length (cm), plant height (cm), straw yield (g/plant), grain yield (g/plant) and thousand grain weight (g). \nDays to flowering were recorded as the number of days from planting when more than 50% of the plants in each plot showed spike emergence. Spike length was recorded after harvesting randomly selected three spikes of each genotype.  \nFlag leaf area was measured at the flowering stage using the formula Leaf Area = Length × Maximum Width × 0.75.\nPeduncle length was measured from the flag leaf node to the base of the spike (excluding the spike itself) on the main tiller of each plant. \nPlant height was recorded at physiological maturity as the average three of randomly selected plants of each genotype by measuring from the soil surface to the spike tip excluding awns.\nDry biomass weight of the above-ground plant material remaining after grain harvest (g/plant).\nGrain yield was recorded as the total weight of grains harvested from each plant at physiological maturity.  (g/plant)\nSeeds was taken from the harvested and dried grains. 1000 grains were manually counted using a seed counter then weighed using a precision electronic balance.  (g)","metadataDate":"2026-06-29T18:07:12","notGEMINI":true,"onlineResources":[{"url":"https://data-package.ceh.ac.uk/data/7aa32225-f277-4ff6-bc73-7fe6123748cc","name":"Download the data","description":"Download a copy of this data","function":"download","type":"OTHER"},{"url":"https://data-package.ceh.ac.uk/sd/7aa32225-f277-4ff6-bc73-7fe6123748cc.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"pointsOfContact":[{"familyName":"Prins","givenName":"A.","organisationName":"Rothamsted Research","organisationIdentifier":"https://ror.org/0347fy350","role":"pointOfContact","email":"anneke.prins@rothamsted.ac.uk","fullName":"Prins, A.","pointOfContact":"Rothamsted Research"}],"publicationDate":"2026-01-06T00:00:00.000Z","publishers":[{"organisationName":"NERC EDS Environmental Information Data 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