{"accessLimitation":{"value":"noLimitations","availability":"Available","description":"Access to the resource is not subject to any access limitations and is available to all users without restriction.","uri":"http://vocab.nerc.ac.uk/collection/N07/current/UNRS/"},"authors":[{"familyName":"Erezyilmaz","givenName":"D.F.","organisationName":"University of Oxford","organisationIdentifier":"https://ror.org/052gg0110","role":"author","email":"deniz.erezyilmaz@dpag.ox.ac.uk","nameIdentifier":"https://orcid.org/0000-0001-8416-8561","fullName":"Erezyilmaz, D.F."},{"familyName":"Lu","givenName":"K.","organisationName":"State University of New York","organisationIdentifier":"https://ror.org/01q1z8k08","role":"author","email":"kenneth81891@yahoo.com","fullName":"Lu, K."}],"availability":"Available","citation":{"authors":["Erezyilmaz, D.F.","Lu, K."],"bibtex":"https://catalogue.ceh.ac.uk/documents/871c537d-2a84-432f-8597-7e895e0ffdcb/citation?format=bib","day":13,"doi":"10.5285/871c537d-2a84-432f-8597-7e895e0ffdcb","month":4,"publisher":"NERC EDS Environmental Information Data Centre","resourceTypeGeneral":"nonGeographicDataset","ris":"https://catalogue.ceh.ac.uk/documents/871c537d-2a84-432f-8597-7e895e0ffdcb/citation?format=ris","title":"Genotype-phenotype association for mate choice in a Drosophila simulans-sechellia backcross population","url":"https://doi.org/10.5285/871c537d-2a84-432f-8597-7e895e0ffdcb","year":2022},"custodians":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"}],"datasetReferenceDate":{"publicationDate":"2022-04-13","releasedDate":"2022-05-01"},"description":"The data resource contains Drosophila simulans-sechellia backcross female mate choice (phenotype) aligned with whole-genome genotypes of 692 female backcross progeny. The data was generated in a laboratory at Stony Brook University New York and each test was conducted in 28.5 x 95mm plastic vials. Phenotype data was collected in 2015 and the final analysis was completed in 2021. Illumina libraries were sequenced at University of Oregon G3 Genomics Center, and the multiplexed shotgun genotyping (MSG) software was run at Janelia Research Campus Virginia.\n\nThe experiments were conducted to align the genotype of females with behavioural phenotype, female preference. Three test females were allowed to choose from three males of either species. ‘Choice’ was determined by copulation, and the species of ‘chosen’ male was recorded before gDNA was extracted from the female. \n\nStandard molecular biology was used to generate Illumina libraries from individual backcross females for sequencing. Deniz Erezyilmaz was responsible for data collection and interpretation. Those genomes with ambiguous genotypes at more than 25% of markers due to sparse reads/coverage were eliminated from the analysis.\n\nThe research was funded through NERC grant NE/S010351/1. Investigating the dual role of mate choice genes in behavioural isolation and hybridization.","distributionFormats":[{"name":"Comma-separated values (CSV)","type":"text/csv","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"funding":[{"funderName":"Natural Environment Research Council","funderIdentifier":"https://ror.org/02b5d8509","awardTitle":"Investigating the dual role of mate choice genes in behavioural isolation and hybridization","awardNumber":"NE/S010351/1","awardURI":"https://gtr.ukri.org/search/project?term=NE%2FS010351%2F1","orcid":false,"ror":true}],"id":"871c537d-2a84-432f-8597-7e895e0ffdcb","incomingCitationCount":0,"infoLinks":[{"url":"https://data-package.ceh.ac.uk/sd/871c537d-2a84-432f-8597-7e895e0ffdcb.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"keywordsOther":[{"value":"Drosophila simulans","uri":"http://www.wikidata.org/entity/Q310061"},{"value":"Drosophila sechellia ","uri":"http://www.wikidata.org/entity/Q3040032"}],"licences":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}],"lineage":"The Drosophila simulans-sechellia backcross genotypes were obtained by Multiplexed Shotgun Genotyping (Andolfatto et al, 2011), and the files were thinned using David Stern’s (Janelia Research Campus) script, pull_thin (github.com/dstern/pull_thin) were obtained by two-choice assay, females choosing between D. simulans and D. sechellia males.\n\nStandard molecular biology was used to generate Illumina libraries from individual backcross females for sequencing, as described in: Andolfatto, P., Davison, D., Erezyilmaz, D., Hu, T.T., Mast, J., Sunayama-Morita, T., & Stern, D.L. (2011). Multiplexed shotgun genotyping for rapid and efficient genetic mapping. In Genome Research (Vol. 21, Issue 4, pp. 610–617). Cold Spring Harbor Laboratory. https://doi.org/10.1101/gr.115402.110","metadataDate":"2026-06-29T16:10:01","notGEMINI":false,"onlineResources":[{"url":"https://data-package.ceh.ac.uk/data/871c537d-2a84-432f-8597-7e895e0ffdcb","name":"Download the data","description":"Download a copy of this data","function":"download","type":"OTHER"},{"url":"https://data-package.ceh.ac.uk/sd/871c537d-2a84-432f-8597-7e895e0ffdcb.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"pointsOfContact":[{"honorificPrefix":"Dr","familyName":"Erezyilmaz","givenName":"Deniz","organisationName":"University of Oxford","organisationIdentifier":"https://ror.org/052gg0110","role":"pointOfContact","email":"deniz.erezyilmaz@dpag.ox.ac.uk","nameIdentifier":"https://orcid.org/0000-0001-8416-8561","fullName":"Erezyilmaz, D.","pointOfContact":"University of Oxford"}],"publicationDate":"2022-04-13T00:00:00.000Z","publishers":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"}],"relationships":[{"relation":"http://purl.org/dc/terms/isPartOf","target":"8cc21ce8-662c-470e-8b61-9bfeef9a1f05"}],"resourceIdentifiers":[{"code":"https://catalogue.ceh.ac.uk/id/871c537d-2a84-432f-8597-7e895e0ffdcb"},{"code":"10.5285/871c537d-2a84-432f-8597-7e895e0ffdcb","codeSpace":"doi"}],"resourceMaintenance":[{"frequencyOfUpdate":"notPlanned"}],"resourceType":{"value":"nonGeographicDataset"},"responsibleParties":[{"honorificPrefix":"Dr","familyName":"Erezyilmaz","givenName":"Deniz","organisationName":"University of Oxford","organisationIdentifier":"https://ror.org/052gg0110","role":"pointOfContact","email":"deniz.erezyilmaz@dpag.ox.ac.uk","nameIdentifier":"https://orcid.org/0000-0001-8416-8561","fullName":"Erezyilmaz, D.","pointOfContact":"University of Oxford"},{"familyName":"Erezyilmaz","givenName":"D.F.","organisationName":"University of Oxford","organisationIdentifier":"https://ror.org/052gg0110","role":"author","email":"deniz.erezyilmaz@dpag.ox.ac.uk","nameIdentifier":"https://orcid.org/0000-0001-8416-8561","fullName":"Erezyilmaz, D.F."},{"familyName":"Lu","givenName":"K.","organisationName":"State University of New York","organisationIdentifier":"https://ror.org/01q1z8k08","role":"author","email":"kenneth81891@yahoo.com","fullName":"Lu, K."},{"familyName":"Erezyilmaz","givenName":"D.F.","organisationName":"University of Oxford","organisationIdentifier":"https://ror.org/052gg0110","role":"rightsHolder","email":"deniz.erezyilmaz@dpag.ox.ac.uk","fullName":"Erezyilmaz, D.F."},{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"},{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"}],"rightsHolders":[{"familyName":"Erezyilmaz","givenName":"D.F.","organisationName":"University of Oxford","organisationIdentifier":"https://ror.org/052gg0110","role":"rightsHolder","email":"deniz.erezyilmaz@dpag.ox.ac.uk","fullName":"Erezyilmaz, D.F."}],"supplemental":[{"description":"Andolfatto, P., Davison, D., Erezyilmaz, D., Hu, T.T., Mast, J., Sunayama-Morita, T., & Stern, D.L. (2011). Multiplexed shotgun genotyping for rapid and efficient genetic mapping. In Genome Research (Vol. 21, Issue 4, pp. 610–617). Cold Spring Harbor Laboratory.","url":"https://doi.org/10.1101/gr.115402.110","function":"relatedArticle"}],"title":"Genotype-phenotype association for mate choice in a Drosophila simulans-sechellia backcross population","topicCategories":[{"value":"biota","uri":"http://inspire.ec.europa.eu/metadata-codelist/TopicCategory/biota"}],"type":"nonGeographicDataset","uri":"https://catalogue.ceh.ac.uk/id/871c537d-2a84-432f-8597-7e895e0ffdcb","useConstraints":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}]}