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:8dde3529-1cf7-4b0f-907d-a1631f38afd7
dcterms:title "Genotype-phenotype association for mate choice in progeny of Drosophila simulans backcross" ;
dcterms:identifier "https://catalogue.ceh.ac.uk/id/8dde3529-1cf7-4b0f-907d-a1631f38afd7","https://doi.org/10.5285/8dde3529-1cf7-4b0f-907d-a1631f38afd7" ;
dcterms:bibliographicCitation "Erezyilmaz, D.F., Lu, K. (2022). Genotype-phenotype association for mate choice in progeny of Drosophila simulans backcross. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/8dde3529-1cf7-4b0f-907d-a1631f38afd7" ;
dcterms:description "The data resource contains Drosophila simulans backcross female mate choice (phenotype) aligned with whole-genome genotypes of 784 female backcross progeny. The data were generated in a laboratory at Stony Brook University, New York and each test was conducted in 28.5 x 95mm plastic vials. Phenotype data was collected in 2015 and the final analysis was completed in 2021. Illumina libraries were sequenced at University of Oregon G3 Genomics Center, and the multiplexed shotgun genotyping (MSG) software was run at Janelia Research Campus, Virginia. The experiments were conducted to align the genotype of females with behavioural phenotype, female preference. Three test females were allowed to choose from three males of either species. ‘Choice’ was determined by copulation, and the species of ‘chosen’ male was recorded before gDNA was extracted from the female. Standard molecular biology was used to generate Illumina libraries from individual backcross females for sequencing. Deniz Erezyilmaz was responsible for data collection and interpretation. Those genomes with incomplete or inconsistent genomes due to sparse reads/coverage were eliminated from the analysis. The research was funded through NERC grant NE/S010351/1. Investigating the dual role of mate choice genes in behavioural isolation and hybridization." ;
dcterms:provenance [
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rdfs:label "The Drosophila simulans backcross genotypes were obtained by Multiplexed Shotgun Genotyping (Andolfatto et al, 2011), and the files were thinned using David Stern’s (Janelia Research Campus) script, pull_thin (Github) were obtained by two-choice assay, females choosing between D. simulans and D. sechellia males. Standard molecular biology was used to generate Illumina libraries from individual backcross females for sequencing., as described in Andolfatto, P., Davison, D., Erezyilmaz, D., Hu, T.T., Mast, J., Sunayama-Morita, T., & Stern, D.L. (2011). Multiplexed shotgun genotyping for rapid and efficient genetic mapping. In Genome Research (Vol. 21, Issue 4, pp. 610–617). Cold Spring Harbor Laboratory. https://doi.org/10.1101/gr.115402.110"
] ;
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<https://doi.org/10.1093/g3journal/jkae279> ;
dcterms:subject
"QTL analysis",
"Assortative Hybridization",
"Quantitative trait locus (QTL) analysis",
<http://www.eionet.europa.eu/gemet/concept/4080>,
<http://vocabs.lter-europe.net/EnvThes/21028>,
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foaf:name "Erezyilmaz, D." ;
foaf:familyName "Erezyilmaz" ;
foaf:givenName "Deniz" ;
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dcterms:bibliographicCitation "Lu, K., & Erezyilmaz, D. (2024). Two major-effect loci influence interspecific mating in females of the sibling species, Drosophila simulans and D. sechellia. In S. Macdonald (Ed.), G3: Genes, Genomes, Genetics. Oxford University Press (OUP)";
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