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","organisationName":"University of California Riverside","role":"author","email":"van.moresco@gmail.com","address":{"country":"United States of America"},"fullName":"Moresco, V."},{"familyName":"Ormsby","givenName":"M.J.","organisationName":"University of Stirling","role":"author","email":"Michael.ormsby1@stir.ac.uk","fullName":"Ormsby, M.J."},{"familyName":"Oliver","givenName":"D.O.","organisationName":"University of Stirling","role":"author","email":"David.oliver@stir.ac.uk","fullName":"Oliver, D.O."},{"familyName":"Quilliam","givenName":"R. ","organisationName":"University of Stirling","role":"author","email":"Richard.quilliam@stir.ac.uk","nameIdentifier":"https://orcid.org/0000-0001-7020-4410","fullName":"Quilliam, R."}],"availability":"Available","boundingBoxes":[{"westBoundLongitude":-3.878,"eastBoundLongitude":-2.747,"southBoundLatitude":55.848,"northBoundLatitude":56.277,"bounds":"{\"type\": \"Feature\",      \"properties\": {},      \"geometry\": {        \"type\": \"Polygon\",        \"coordinates\": [[[-3.878, 55.848], [-3.878, 56.277], [-2.747, 56.277], [-2.747, 55.848], [-3.878, 55.848]]]      }}","coordinates":"[[[-3.878, 55.848], [-3.878, 56.277], [-2.747, 56.277], [-2.747, 55.848], [-3.878, 55.848]]]"}],"citation":{"authors":["Metcalf, R.","White, H.L.","Moresco, V.","Ormsby, M.J.","Oliver, D.O.","Quilliam, R."],"bibtex":"https://catalogue.ceh.ac.uk/documents/9d0a6437-f01e-4cf3-9f83-86d2f4ead869/citation?format=bib","day":9,"doi":"10.5285/9d0a6437-f01e-4cf3-9f83-86d2f4ead869","month":8,"publisher":"NERC EDS Environmental Information Data Centre","resourceTypeGeneral":"dataset","ris":"https://catalogue.ceh.ac.uk/documents/9d0a6437-f01e-4cf3-9f83-86d2f4ead869/citation?format=ris","title":"Concentrations and antibiotic resistance of faecal indicator organisms and human pathogens associated with sewage waste on Scottish beaches, 2021","url":"https://doi.org/10.5285/9d0a6437-f01e-4cf3-9f83-86d2f4ead869","year":2024},"custodians":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"}],"datasetReferenceDate":{"publicationDate":"2024-08-09"},"description":"The data includes concentrations of faecal indicator organisms isolated from the plastic waste and natural substrates found on Scottish beaches, as well as the antibiotic resistance of selected strains of these organisms.\nSewage-associated plastic waste, and naturally occurring substrates (seaweed and sand), were collected in 2021 from ten beaches along the Firth of Forth estuary (Scotland, UK). The concentrations of faecal indicator organisms (FIOs), E. coli and intestinal enterococci (IE), and potential human pathogens (Vibrio spp.) on the plastic waste and natural substrates were  analysed using selective media. Minimum inhibitory concentration (MIC) analysis was used to determine antibiotic resistance in selected strains.\n","distributionFormats":[{"name":"Comma-separated values (CSV)","type":"text/csv","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"id":"9d0a6437-f01e-4cf3-9f83-86d2f4ead869","incomingCitationCount":0,"infoLinks":[{"url":"https://data-package.ceh.ac.uk/sd/9d0a6437-f01e-4cf3-9f83-86d2f4ead869.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"inspireThemes":[{"theme":"Habitats and Biotopes","uri":"http://inspire.ec.europa.eu/theme/hb"}],"keywordsOther":[{"value":"Beach pollution"},{"value":"Environmental pathogens"},{"value":"Plastic pollution"},{"value":"Plastisphere"},{"value":"Sewage discharge"},{"value":"Wet wipes"}],"licences":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}],"lineage":"Sewage-associated plastic waste (wipes, cotton bud sticks and sanitary products) was collected from ten beaches. All samples were stored at 4 °C and processed within 48 h.\nReplicate composite samples of wipes, cotton bud sticks and seaweed from each site were pre-enriched in non-selective media Luria-Bertani (LB) broth or Vibrio selective Bile Peptone broth at 37 °C in a shaking incubator for 18 h. Following pre-enrichment, serial dilutions were carried out in phosphate buffered saline (PBS) and dilutions were plated onto selective media. All plates were inverted and incubated. The presence or absence of target bacteria on all sewage-associated plastic waste samples was determined by enumerating colony forming units (CFUs).\nMinimum inhibitory concentration (MIC): Representative individual colonies of E. coli, IE and Vibrio spp. isolated from seaweed, wet wipe, and cotton bud stick samples, were picked off agar plates, grown further overnight in LB broth, and subjected to MIC analysis to determine antibiotic resistance to  amoxicillin, ampicillin, streptomycin, cephalexin, and tetracycline at varying concentrations. Two-fold serial dilutions of each antibiotic were made in LB broth in 96-well plates. Bacterial cultures were adjusted to an optical density at a wavelength of 600nm (OD600nm) of 0.1, and 10 μL added to each well of a 96-well plate. Samples were incubated overnight at 37 °C.\n","metadataDate":"2026-06-29T15:43:10","notGEMINI":false,"onlineResources":[{"url":"https://data-package.ceh.ac.uk/data/9d0a6437-f01e-4cf3-9f83-86d2f4ead869","name":"Download the data","description":"Download a copy of this data","function":"download","type":"OTHER"},{"url":"https://data-package.ceh.ac.uk/sd/9d0a6437-f01e-4cf3-9f83-86d2f4ead869.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"pointsOfContact":[{"familyName":"Metcalf","givenName":"R.","organisationName":"University of Stirling","role":"pointOfContact","email":"Rebecca.metcalf@stir.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-1463-427X","address":{"country":"United Kingdom"},"fullName":"Metcalf, R.","pointOfContact":"University of Stirling"}],"publicationDate":"2024-08-09T00:00:00.000Z","publishers":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"}],"relationships":[{"relation":"http://purl.org/dc/terms/relation","target":"fdd72810-a57f-444f-83cf-f2351ef4c940"},{"relation":"http://purl.org/dc/terms/isPartOf","target":"0649e4b5-1bda-467b-9620-bc4c9cb48be4"}],"resourceIdentifiers":[{"code":"https://catalogue.ceh.ac.uk/id/9d0a6437-f01e-4cf3-9f83-86d2f4ead869"},{"code":"10.5285/9d0a6437-f01e-4cf3-9f83-86d2f4ead869","codeSpace":"doi"}],"resourceType":{"value":"dataset"},"responsibleParties":[{"familyName":"Metcalf","givenName":"R.","organisationName":"University of Stirling","role":"author","email":"Rebecca.metcalf@stir.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-1463-427X","fullName":"Metcalf, R."},{"familyName":"White","givenName":"H.L.","organisationName":"University of Stirling","role":"author","email":"Hannah.white@stir.ac.uk","fullName":"White, H.L."},{"familyName":"Moresco","givenName":"V. ","organisationName":"University of California Riverside","role":"author","email":"van.moresco@gmail.com","address":{"country":"United States of America"},"fullName":"Moresco, V."},{"familyName":"Ormsby","givenName":"M.J.","organisationName":"University of Stirling","role":"author","email":"Michael.ormsby1@stir.ac.uk","fullName":"Ormsby, M.J."},{"familyName":"Oliver","givenName":"D.O.","organisationName":"University of Stirling","role":"author","email":"David.oliver@stir.ac.uk","fullName":"Oliver, D.O."},{"familyName":"Quilliam","givenName":"R. ","organisationName":"University of Stirling","role":"author","email":"Richard.quilliam@stir.ac.uk","nameIdentifier":"https://orcid.org/0000-0001-7020-4410","fullName":"Quilliam, R."},{"familyName":"Metcalf","givenName":"R.","organisationName":"University of Stirling","role":"pointOfContact","email":"Rebecca.metcalf@stir.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-1463-427X","address":{"country":"United Kingdom"},"fullName":"Metcalf, R.","pointOfContact":"University of Stirling"},{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"},{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"},{"organisationName":"University of Stirling","role":"rightsHolder"}],"rightsHolders":[{"organisationName":"University of Stirling","role":"rightsHolder"}],"spatialReferenceSystems":[{"code":"http://www.opengis.net/def/crs/EPSG/0/27700","title":"OSGB 1936 / British National Grid"}],"spatialRepresentationTypes":["textTable"],"temporalExtents":[{"begin":"2021-12-01","end":"2021-12-31"}],"title":"Concentrations and antibiotic resistance of faecal indicator organisms and human pathogens associated with sewage waste on Scottish beaches, 2021","topicCategories":[{"value":"environment","uri":"http://inspire.ec.europa.eu/metadata-codelist/TopicCategory/environment"}],"type":"dataset","uri":"https://catalogue.ceh.ac.uk/id/9d0a6437-f01e-4cf3-9f83-86d2f4ead869","useConstraints":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}]}