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dcterms:title "Compositional and growth assay data for tree-hole bacteria communities under differing pH conditions, sampled summer 2021, Wytham Woods, Oxford" ;
dcterms:identifier "https://catalogue.ceh.ac.uk/id/9d64f2bd-2918-416f-b08c-3369edbc1b30","https://doi.org/10.5285/9d64f2bd-2918-416f-b08c-3369edbc1b30" ;
dcterms:bibliographicCitation "Barraclough, T.G., Sant, D.G., Wong, E.L.Y. (2025). Compositional and growth assay data for tree-hole bacteria communities under differing pH conditions, sampled summer 2021, Wytham Woods, Oxford. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/9d64f2bd-2918-416f-b08c-3369edbc1b30" ;
dcterms:description "The data comprises both compositional data and growth assay data from an experiment of wild bacterial communities in Wytham Woods, Oxford. The communities live in puddles of water in tree-holes formed by the roots of beech trees. We manipulated the pH of tree-holes during the experiment for comparison with control tree-holes that were unmanipulated. Compositional data comprises the frequency of different Amplicon Sequencing Variants and derived measures of species diversity for samples taken over time (up to 12 weeks from the start of the experiment). The growth assay data comprises measurements of growth rates of community samples and isolates back in the laboratory, when grown on beech tea media manipulated to have different pHs." ;
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rdfs:label "A field experiment was conducted in summer 2021 that surveyed bacterial communities in tree-hole puddles formed by beech tree roots at Wytham Woods, Oxford. The experiment added lime (alkali) to a set of tree-holes and compared changes in bacterial communities to control tree-holes without liming. Samples were collected before and after the manipulation, and some of these were used for growth assays in the laboratory: growing different combinations of isolates and communities on beech tea medium with modified pH."
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