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:acbe4aa4-4ed9-428a-b633-ddd1bc119e12
dcterms:title "Feather isotope ratios and sexing of oystercatchers breeding in Iceland, 2013-2017" ;
dcterms:identifier <https://catalogue.ceh.ac.uk/id/acbe4aa4-4ed9-428a-b633-ddd1bc119e12> ;
adms:identifier <https://doi.org/10.5285/acbe4aa4-4ed9-428a-b633-ddd1bc119e12> ;
dcterms:bibliographicCitation "Gill, J.A., Mendez, V., Gunnarsson, T.G., Alves, J.A., Thorrison, B. (2020). Feather isotope ratios and sexing of oystercatchers breeding in Iceland, 2013-2017. NERC Environmental Information Data Centre. https://doi.org/10.5285/acbe4aa4-4ed9-428a-b633-ddd1bc119e12" ;
dcterms:description "Dataset comprises of the delta-13C and delta-15N stable isotopic information from feather samples (for 552 individuals) and the sex (assigned by DNA-analysis of blood samples for 321 individuals) of oystercatchers (Haematopus ostralegus) breeding in Iceland during the summers of 2013-2017. The Icelandic oystercatcher population contains individuals that stay in Iceland year-round and individuals that migrate to mainland Europe in the non-breeding season, and feather isotope ratios provide a means of distinguishing between these migratory behaviours (as confirmed by observations of marked individuals). These data were collected by a collaborative team from the University of Iceland, University of East Anglia (UK) and the University of Aveiro (Portugal)." ;
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rdfs:label "Feathers were washed in a 2:1 chloroform/methanol solution, left to dry overnight in a fume cupboard and cut into small fragments. Each sample was then weighed and loaded into a combustion Costech elemental analyser coupled to a Thermo Scientific Delta XP continuous flow isotopic ratio mass spectrometer and a Conflo III interface. DNA was extracted from blood samples using a standard ammonium acetate technique (dx.doi.org/10.17504/protocols.io.knycvfw) and diluted to a working concentration of 10–50 ng/μL, and used to assign sex following the methods set out in Fridolfsson & Ellegren (1999: DOI: 10.2307/3677252)."
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