Temperature and copper effects on the nematode Caenorhabditis elegans
bibliographic citation: Cedergreen, N., Nørhave, N.J., Svendsen, C., Spurgeon, D.J. (2015). Temperature and copper effects on the nematode Caenorhabditis elegans . NERC Environmental Information Data Centre. https://doi.org/10.5285/af125e27-3b70-4f0a-81fb-a7eb10f64ef7
description: Data comprise body length (micrometres) of nematode (Caenorhabditis elegans) offspring from a laboratory study in which animals were exposed to control (0 copper) or copper dosed agar at different average temperatures (8 to 24 °C)) and under fluctuation conditions of low (plus or minus 4 °C) and high (plus or minus 8 °C) amplitude (average temperatures of 12, 16, 20 °C and 16 °C respectively)
provenance: ProvenanceStatement
  label: Experimental design Culture conditions for test organism: C. elegans (N2 Bristol strain) from the C. elegans Genetics Centre, University of Minnesota) were cultivated in darkness at 20°C on nematode growth medium (NGM) agar plates and fed Escherichia coli of uracil deficient strain OP50. At least 2 weeks before the start of all experiments new cultures were initiated at the selected test temperatures to acclimatize the worms through several generations. Cultures were maintained by transferring a chunk of agar and associated eggs, juvenile and adult worms from an existing culture to freshly prepared NGM agar plates weekly. Set-up of toxicity tests: Exposures were conducted at steady temperatures of 8, 12, 16, 20 and 24°C and variable temperature scenarios with daily fluctuations of +/- 4°C in the ranges 8-16°C (12°C average), 12-20°C (16°C average) and 16-24°C (20°C average) and daily fluctuations of +/- 8°C in the ranges 8-24°C (16°C average). Temperatures were changed with a rate of 4°C per hour. Separate toxicity tests with copper were conducted under all temperature regimes using concentration known to result in changes in measured traits. Copper concentrations used were 0, 1, 3, 8, 20 and 40 mg Cu/L agar. The highest concentrations equate to levels 2-3 orders of magnitude greater than those found in surface waters and soil pore waters from uncontaminated sites, but are comparable to soil pore water concentrations in heavily polluted soils. A stock solution of CuCl2 in demineralized water (2 g Cu/L) was made and used throughout the experiment. This copper stock solution was added to the NGM agar while liquid and mixed. Batches of NGM were produced every five days during the course of the experiment to minimize immobilization of copper in the agar. All tests were conducted using synchronized cohorts of nematodes produced from adults selected from the main stock cultures acclimatized at each temperature scenario. To generate these cohorts, adult hermaphrodite worms…
temporal: PeriodOfTime
  start date: 2013-06-01
  end date: 2013-10-31
contact point: Svendsen, C.
publisher: NERC Environmental Information Data Centre
is referenced by: journal.pone.0140277
subject: Environmental risk
subject: Pollution
subject: Caenorhabditis elegans
type: Dataset
available: 2015-10-06
landing page: https://doi.org/10.5285/af125e27-3b70-4f0a-81fb-a7eb10f64ef7
license: OGL-UK-3.0.ttl
rights: RightsStatement
  copyright notice: copyright UK Centre for Ecology & Hydrology
access rights: UNRS
theme: Environmental risk
theme: Pollution
distribution:
  access URL: https://data-package.ceh.ac.uk/data/af125e27-3b70-4f0a-81fb-a7eb10f64ef7
  license: OGL-UK-3.0.ttl
  rights: RightsStatement
    copyright notice: copyright UK Centre for Ecology & Hydrology
  access rights: UNRS
  format: Comma-separated values (CSV)
creator: Spurgeon, D.J.
language: en

Svendsen, C.
type: Person
family name: Svendsen
given name: Claus
member: UK Centre for Ecology & Hydrology

NERC Environmental Information Data Centre
type: Organization

Cedergreen, N.
type: Person
family name: Cedergreen
given name: N.
member: University of Copenhagen

Nørhave, N.J.
type: Person
family name: Nørhave
given name: N.J.
member: University of Copenhagen

Svendsen, C.
type: Person
family name: Svendsen
given name: C.
member: UK Centre for Ecology & Hydrology

Spurgeon, D.J.
type: Person
family name: Spurgeon
given name: D.J.
member: UK Centre for Ecology & Hydrology

journal.pone.0140277
type: Expression
bibliographic citation: Cedergreen, N., Nørhave, N.J., Svendsen, C., & Spurgeon, D.J. (2016). Variable Temperature Stress in the Nematode Caenorhabditis elegans (Maupas) and Its Implications for Sensitivity to an Additional Chemical Stressor. PLOS ONE, 11(1), e0140277.

Environmental risk
type: Concept

Pollution
type: Concept

Caenorhabditis elegans
type: Concept

UK Centre for Ecology & Hydrology
type: Organization

University of Copenhagen
type: Organization