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","organisationName":"Natural History Museum, University of Oslo, Norway","role":"author","email":"rafale.assis@gmail.com","fullName":"Assis, R.L."},{"familyName":"Hartley","givenName":"I.P.","organisationName":"University of Exeter","role":"author","email":"I.Hartley@exeter.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-9183-6617","fullName":"Hartley, I.P."}],"availability":"Available","boundingBoxes":[{"westBoundLongitude":-68.5,"eastBoundLongitude":-31.7,"southBoundLatitude":-24.6,"northBoundLatitude":5.1,"bounds":"{\"type\": \"Feature\",      \"properties\": {},      \"geometry\": {        \"type\": \"Polygon\",        \"coordinates\": [[[-68.5, -24.6], [-68.5, 5.1], [-31.7, 5.1], [-31.7, -24.6], [-68.5, -24.6]]]      }}","coordinates":"[[[-68.5, -24.6], [-68.5, 5.1], [-31.7, 5.1], [-31.7, -24.6], [-68.5, -24.6]]]"}],"citation":{"authors":["Lugli, L.F.","Quesada, C.A.N.","Andersen, K.M.","Mercado, L.M.","Di Ponzio, R.","Ribeiro, G.","Assis, R.L.","Hartley, I.P."],"bibtex":"https://catalogue.ceh.ac.uk/documents/b9a2523e-09ba-43c7-acfa-a894a231b133/citation?format=bib","day":2,"doi":"10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133","month":4,"publisher":"NERC Environmental Information Data Centre","resourceTypeGeneral":"dataset","ris":"https://catalogue.ceh.ac.uk/documents/b9a2523e-09ba-43c7-acfa-a894a231b133/citation?format=ris","title":"Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018","url":"https://doi.org/10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133","year":2020},"custodians":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"}],"datasetReferenceDate":{"publicationDate":"2020-04-02"},"description":"The data consists of potential activity of enzymes released by microorganisms in soils of old growth forests in the Central Amazon. Soils were sampled in January 2018, seven months after nutrient addition in a large-scale nutrient fertilisation experiment. Enzyme activities are given as a mean of the microbial community per plot, where five points inside each plot were sampled in the 0-5 cm and 5-10 cm soil layers. The dataset  depicts the potential activities of the following enzymes: ß-glucosidase, N-acetyl ß-glucosaminidase and phosphomonoesterase at two different soil depths. \n\nSamples were collected at the AFEX project area in Manaus, Brazil at the Biological Dynamics of Forest Fragments Project (BDFFP/ INPA). \n\nThe study was funded by NERC, BDFFP (logistical support) and Brazilian government (student scholarship).","distributionFormats":[{"name":"Comma-separated values (CSV)","type":"text/csv","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"funding":[{"funderName":"Natural Environment Research Council","funderIdentifier":"https://ror.org/02b5d8509","orcid":false,"ror":true}],"id":"b9a2523e-09ba-43c7-acfa-a894a231b133","incomingCitationCount":0,"infoLinks":[{"url":"https://data-package.ceh.ac.uk/sd/b9a2523e-09ba-43c7-acfa-a894a231b133.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"keywordsOther":[{"value":"soil","uri":"http://www.eionet.europa.eu/gemet/concept/7843"},{"value":"fertiliser","uri":"http://www.eionet.europa.eu/gemet/concept/3169"}],"keywordsProject":[{"value":"Biological Dynamics of Forest Fragments Project (BDFFP)"}],"keywordsTheme":[{"value":"Soil","uri":"http://onto.nerc.ac.uk/CEHMD/topic/17"},{"value":"Environmental survey","uri":"http://onto.nerc.ac.uk/CEHMD/topic/7"}],"licences":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}],"lineage":"Potential activities of 4-Methylumbelliferyl-β-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS), enzymes involved in the cycling of carbon, nitrogen and phosphorus were measured using microplate fluorimetric assays. After centrifugation with buffer solution, samples were incubated for one hour using 4-Methylumbelliferyl-β-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS) as substrates. Fluorescence was read on a fluorometer (Tecan Infinite® 200 PRO, Grödig, Austria), at 365 nm excitation and 450 nm emission. Enzyme activities are presented in nmol MUF g soil dry weight-1 hour-1. ","metadataDate":"2026-06-29T18:19:36","notGEMINI":true,"onlineResources":[{"url":"https://data-package.ceh.ac.uk/data/b9a2523e-09ba-43c7-acfa-a894a231b133","name":"Download the data","description":"Download a copy of this data","function":"download","type":"OTHER"},{"url":"https://data-package.ceh.ac.uk/sd/b9a2523e-09ba-43c7-acfa-a894a231b133.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"pointsOfContact":[{"familyName":"Lugli","givenName":"Laynara","organisationName":"National Institute of Amazonian Research, Brazil","role":"pointOfContact","email":"laynaralugli@gmail.com","fullName":"Lugli, L.","pointOfContact":"National Institute of Amazonian Research, Brazil"},{"honorificPrefix":"Professor","familyName":"Hartley","givenName":"Iain","organisationName":"University of Exeter","role":"pointOfContact","email":"I.Hartley@exeter.ac.uk","fullName":"Hartley, I.","pointOfContact":"University of Exeter"}],"publicationDate":"2020-04-02T00:00:00.000Z","publishers":[{"organisationName":"NERC Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"}],"relationships":[{"relation":"http://purl.org/dc/terms/isPartOf","target":"79c9df71-af3b-4ca8-8df8-330fc5fc3507"}],"resourceIdentifiers":[{"code":"https://catalogue.ceh.ac.uk/id/b9a2523e-09ba-43c7-acfa-a894a231b133"},{"code":"10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133","codeSpace":"doi"}],"resourceType":{"value":"dataset"},"responsibleParties":[{"familyName":"Lugli","givenName":"L.F.","organisationName":"National Institute of Amazonian Research, Brazil","role":"author","email":"laynaralugli@gmail.com","nameIdentifier":"https://orcid.org/0000-0001-8404-4841","fullName":"Lugli, L.F."},{"familyName":"Quesada","givenName":"C.A.N.","organisationName":"National Institute of Amazonian Research, Brazil","role":"author","email":"quesada.beto@gmail.com","fullName":"Quesada, C.A.N."},{"familyName":"Andersen","givenName":"K.M.","organisationName":"Nanyang Technological University, Singapore","role":"author","email":"kelly.andersen@ntu.edu.sg","nameIdentifier":"https://orcid.org/0000-0002-1460-9904","fullName":"Andersen, K.M."},{"familyName":"Mercado","givenName":"L. 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