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The sampling sites were determined from maps and in field to provide good spatial coverage of the Wold River through to the Tamar River. A 5 litre bucket was filled with stream water. An Ultrameter multiprobe was used to measure water temperature, conductivity, Ph and organic reactive phosphorous (ORP) in the field. A 300 ml sample of water from the bucket was taken in a plastic bottle, constituting the 'unfiltered' sample. A second 300ml bottle was filled with water filtered through a syringe and filter (GFC 0.45­μm),constituting the 'filtered' sample. Samples were kept in the cold and removed to the laboratory for analyses. 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