{"accessLimitation":{"value":"noLimitations","availability":"Available","description":"Access to the resource is not subject to any access limitations and is available to all users without restriction.","uri":"http://vocab.nerc.ac.uk/collection/N07/current/UNRS/"},"authors":[{"familyName":"Montgomery","givenName":"S.","organisationName":"University of Bristol","organisationIdentifier":"https://ror.org/0524sp257","role":"author","email":"s.montgomery@bristol.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-5474-5695","address":{"deliveryPoint":"Senate House, Tyndall Avenue","city":"Bristol","postalCode":"BS8 1TH","country":"United Kingdom"},"fullName":"Montgomery, S."},{"familyName":"Couto","givenName":"A.","organisationName":"University of Bristol","organisationIdentifier":"https://ror.org/0524sp257","role":"author","email":"antoine.couto@universite-paris-saclay.fr","nameIdentifier":"https://orcid.org/0000-0002-5528-7331","address":{"deliveryPoint":"Senate House, Tyndall Avenue","city":"Bristol","postalCode":"BS8 1TH","country":"United Kingdom"},"fullName":"Couto, A."}],"availability":"Available","boundingBoxes":[{"westBoundLongitude":-119.308,"eastBoundLongitude":-33.879,"southBoundLatitude":-56.207,"northBoundLatitude":33.669,"bounds":"{\"type\": \"Feature\",      \"properties\": {},      \"geometry\": {        \"type\": \"Polygon\",        \"coordinates\": [[[-119.308, -56.207], [-119.308, 33.669], [-33.879, 33.669], [-33.879, -56.207], [-119.308, -56.207]]]      }}","coordinates":"[[[-119.308, -56.207], [-119.308, 33.669], [-33.879, 33.669], [-33.879, -56.207], [-119.308, -56.207]]]"}],"citation":{"authors":["Montgomery, S.","Couto, A."],"bibtex":"https://catalogue.ceh.ac.uk/documents/de62eff7-6ea4-44d7-a015-2eb9b25cf882/citation?format=bib","day":15,"doi":"10.5285/de62eff7-6ea4-44d7-a015-2eb9b25cf882","month":11,"publisher":"NERC EDS Environmental Information Data Centre","resourceTypeGeneral":"dataset","ris":"https://catalogue.ceh.ac.uk/documents/de62eff7-6ea4-44d7-a015-2eb9b25cf882/citation?format=ris","title":"Taxonomic data, neuropil volume measurements, and mushroom body calyx kenyon cell and synapse counts for Heliconiini butterflies, Central and South America, 2012-2016","url":"https://doi.org/10.5285/de62eff7-6ea4-44d7-a015-2eb9b25cf882","year":2024},"custodians":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"}],"datasetReferenceDate":{"publicationDate":"2024-11-15"},"description":"This dataset contains taxanomic and neuropil volume measurements of wild caught heliconiine butterflies, as well as kenyon cell and synapse counts in the mushroom body calyx of insectory -reared heliconiine butterflies. \nWild caught butterflies were sampled from five locations in Central and South America between 2012 and 2016. Locations included Costa Rica, Panama, French Guiana, Ecuador, and Peru. These locations covered a range of habitats from dry to wet forest, at elevations from sea level to 2500m. Interspecific data from wild caught individuals include identification to a species level, sex, and body size, as well as volume measurements of the mushroom body structures, central brain, medulla, and antennal lobe. \n\nMeasurements of mushroom body calyx volume, and estimates of Kenyon cell and synapse counts are included for six species of  insectary-reared adult heliconiine butterflies (Agraulis vanilla, dryas iulia, eueides Isabella, heliconius melpmonene, heliconius Hortense, heliconius hecale). Two species (dryas iulia, heliconius charthonia) were also sampled for microglomeruli counts.\nThis dataset was created as part of a NERC Independent Research Fellowship NE/N014936/1, to analyse the evolutionary expansions in mushroom body size in Heliconius butterflies, compared to their closest relatives.\n","distributionFormats":[{"name":"Comma-separated values (CSV)","type":"text/csv","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"funding":[{"funderName":"Natural Environment Research Council","funderIdentifier":"https://ror.org/02b5d8509","awardTitle":"Neurological adaptation and ecological specialisation","awardNumber":"NE/N014936/2","awardURI":"https://gtr.ukri.org/projects?ref=NE/N014936/2","orcid":false,"ror":true}],"hasOnlineServiceAgreement":true,"id":"de62eff7-6ea4-44d7-a015-2eb9b25cf882","incomingCitationCount":0,"infoLinks":[{"url":"https://data-package.ceh.ac.uk/sd/de62eff7-6ea4-44d7-a015-2eb9b25cf882.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"inspireThemes":[{"theme":"Species Distribution","uri":"http://inspire.ec.europa.eu/theme/sd"}],"keywordsOther":[{"value":"lepidoptera"},{"value":"butterfly species abundance","uri":"http://vocabs.lter-europe.net/EnvThes/30062"},{"value":"heliconiini"},{"value":"kenyon cell number"},{"value":"brain","uri":"http://vocabs.lter-europe.net/EnvThes/20931"},{"value":"neuropil"},{"value":"mushroom body"},{"value":"mushroom body calyx"},{"value":"synapse density"}],"keywordsTheme":[{"value":"Biodiversity","uri":"http://onto.nerc.ac.uk/CEHMD/topic/2"}],"licences":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}],"lineage":"Samples were collected over multiple field seasons across central and south America using a hand-net, fixed in situ and returned to the UK. Fixed samples were then stained with Anti-SYNORF as a primary antibody, cy2 as a secondary antibody, and imaged using confocal microscopy. The resulting images, which highlight boundaries between brain regions, were then segmented using AMIRA software to quantify neuropil volumes. \n\nInsectary-reared samples were obtained using pupae from commercial supplies. Freshly eclosed butterflies were aged in a controlled temperature room at 28 degrees Celsius with 80% humidity with a 12-hour day/night cycle. The cage was enriched with a natural food resource, supplemented by artificial feeders. Butterflies were sampled six to ten days after emergence.\n\nKenyon cell counts were estimated using immunohistochemical staining against DAPI, imaged with confocal microscopy. Synapse density estimates were made using higher magnification imaging of Anti-SYNORF stained brains, focusing on the calyx. Total count data was obtained by estimating nuclei density/synapse density within sub-sampled regions of a structure, and then extrapolating across the total volume of the structure.\n","metadataDate":"2026-06-29T16:34:43","notGEMINI":false,"onlineResources":[{"url":"https://data-package.ceh.ac.uk/data/de62eff7-6ea4-44d7-a015-2eb9b25cf882","name":"Download the data","description":"Download a copy of this data","function":"download","type":"OTHER"},{"url":"https://data-package.ceh.ac.uk/sd/de62eff7-6ea4-44d7-a015-2eb9b25cf882.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"pointsOfContact":[{"familyName":"Montgomery","givenName":"S.","organisationName":"University of Bristol","organisationIdentifier":"https://ror.org/0524sp257","role":"pointOfContact","email":"s.montgomery@bristol.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-5474-5695","address":{"deliveryPoint":"Senate House, Tyndall Avenue","city":"Bristol","postalCode":"BS8 1TH","country":"United Kingdom"},"fullName":"Montgomery, S.","pointOfContact":"University of Bristol"}],"publicationDate":"2024-11-15T00:00:00.000Z","publishers":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"}],"resourceIdentifiers":[{"code":"https://catalogue.ceh.ac.uk/id/de62eff7-6ea4-44d7-a015-2eb9b25cf882"},{"code":"10.5285/de62eff7-6ea4-44d7-a015-2eb9b25cf882","codeSpace":"doi"}],"resourceType":{"value":"dataset"},"responsibleParties":[{"familyName":"Montgomery","givenName":"S.","organisationName":"University of Bristol","organisationIdentifier":"https://ror.org/0524sp257","role":"pointOfContact","email":"s.montgomery@bristol.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-5474-5695","address":{"deliveryPoint":"Senate House, Tyndall Avenue","city":"Bristol","postalCode":"BS8 1TH","country":"United Kingdom"},"fullName":"Montgomery, S.","pointOfContact":"University of Bristol"},{"familyName":"Montgomery","givenName":"S.","organisationName":"University of Bristol","organisationIdentifier":"https://ror.org/0524sp257","role":"author","email":"s.montgomery@bristol.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-5474-5695","address":{"deliveryPoint":"Senate House, Tyndall Avenue","city":"Bristol","postalCode":"BS8 1TH","country":"United Kingdom"},"fullName":"Montgomery, S."},{"familyName":"Couto","givenName":"A.","organisationName":"University of Bristol","organisationIdentifier":"https://ror.org/0524sp257","role":"author","email":"antoine.couto@universite-paris-saclay.fr","nameIdentifier":"https://orcid.org/0000-0002-5528-7331","address":{"deliveryPoint":"Senate House, Tyndall Avenue","city":"Bristol","postalCode":"BS8 1TH","country":"United Kingdom"},"fullName":"Couto, A."},{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"},{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"},{"organisationName":"University of Bristol","organisationIdentifier":"https://ror.org/0524sp257","role":"rightsHolder","email":"s.montgomery@bristol.ac.uk","address":{"deliveryPoint":"Senate House, Tyndall Avenue","city":"Bristol","administrativeArea":"Lancashire","postalCode":"BS8 1TH","country":"United Kingdom"}}],"rightsHolders":[{"organisationName":"University of Bristol","organisationIdentifier":"https://ror.org/0524sp257","role":"rightsHolder","email":"s.montgomery@bristol.ac.uk","address":{"deliveryPoint":"Senate House, Tyndall Avenue","city":"Bristol","administrativeArea":"Lancashire","postalCode":"BS8 1TH","country":"United Kingdom"}}],"spatialReferenceSystems":[{"code":"http://www.opengis.net/def/crs/EPSG/0/4326","title":"WGS 84"}],"spatialRepresentationTypes":["textTable"],"supplemental":[{"name":" Couto A, Young FJ, Atzeni D, Marty S, Melo‐Flórez L, Hebberecht L, Monllor M, Neal C, Cicconardi F, McMillan WO, Montgomery SH. Rapid expansion and visual specialisation of learning and memory centres in the brains of Heliconiini butterflies. Nature Communications. 2023, 14(1):4024. https://doi.org/10.1038/s41467-023-39618-8","url":"https://doi.org/10.1038/s41467-023-39618-8","function":"relatedArticle"}],"temporalExtents":[{"begin":"2012-01-01","end":"2016-12-31"}],"title":"Taxonomic data, neuropil volume measurements, and mushroom body calyx kenyon cell and synapse counts for Heliconiini butterflies, Central and South America, 2012-2016","topicCategories":[{"value":"biota","uri":"http://inspire.ec.europa.eu/metadata-codelist/TopicCategory/biota"}],"topics":["http://onto.nerc.ac.uk/CEHMD/topic/2"],"type":"dataset","uri":"https://catalogue.ceh.ac.uk/id/de62eff7-6ea4-44d7-a015-2eb9b25cf882","useConstraints":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}]}