{"accessLimitation":{"value":"embargoed","availability":"Embargoed","description":"Access to the resource is restricted until a specified date or the occurrence of a defined condition, after which access may be granted in accordance with applicable policies or licensing conditions","uri":"http://vocab.nerc.ac.uk/collection/N07/current/EMBG/"},"authors":[{"honorificPrefix":"Dr","familyName":"Parker","givenName":"Megan E.","organisationName":"University of Liverpool","organisationIdentifier":"https://ror.org/04xs57h96","role":"author","email":"m.e.parker@liverpool.ac.uk","nameIdentifier":"https://orcid.org/0009-0007-4602-8382","fullName":"Parker, M.E."},{"honorificPrefix":"Dr","familyName":"Zytynska","givenName":"Sharon E.","organisationName":"University of Manchester","organisationIdentifier":"https://ror.org/027m9bs27","role":"author","email":"sharon.zytynska@manchester.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-0174-3303","fullName":"Zytynska, S.E."}],"availability":"Embargoed","citation":{"authors":["Parker, M.E.","Zytynska, S.E."],"bibtex":"https://catalogue.ceh.ac.uk/documents/e65881be-9dca-4111-8a3b-fb84cf78cf15/citation?format=bib","day":13,"doi":"10.5285/e65881be-9dca-4111-8a3b-fb84cf78cf15","month":11,"publisher":"NERC EDS Environmental Information Data Centre","resourceTypeGeneral":"nonGeographicDataset","ris":"https://catalogue.ceh.ac.uk/documents/e65881be-9dca-4111-8a3b-fb84cf78cf15/citation?format=ris","title":"Metabolomic data from the whole-leaf and headspace of barley plants inoculated with rhizobacteria and infested with aphids","url":"https://doi.org/10.5285/e65881be-9dca-4111-8a3b-fb84cf78cf15","year":2025},"custodians":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"}],"datasetReferenceDate":{"creationDate":"2025-08-21","publicationDate":"2025-11-13","releasedDate":"2027-01-04"},"description":"This dataset comprises whole-leaf and headspace metabolomics of barley plants (Hordeum vulgare) that were root-inoculated with three rhizobacterial treatments: Acidovorax radicis N35, Bacillus subtilis B171, or no bacteria (control). Plants were also either infested with Sitobion avenae aphids or left uninfested as controls. Plants were grown under controlled conditions in glasshouse (leaf extracts) or climate chamber (headspace analysis). \n\nThe purpose of these experiments was to compare metabolomic profiles across rhizobacterial treatments, and when aphids were feeding on plants, as an assessment of wider-community effects on rhizosphere inoculation of barley plants.","distributionFormats":[{"name":"Comma-separated values (CSV)","type":"text/csv","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"funding":[{"funderName":"Natural Environment Research Council","funderIdentifier":"https://ror.org/02b5d8509","awardNumber":"NE/X011763/1","orcid":false,"ror":true}],"hasOnlineServiceAgreement":true,"id":"e65881be-9dca-4111-8a3b-fb84cf78cf15","incomingCitationCount":0,"keywordsOther":[{"value":"plant ecology","uri":"http://www.eionet.europa.eu/gemet/concept/8910"},{"value":"chemical analysis","uri":"http://www.eionet.europa.eu/gemet/concept/1298"},{"value":"insect","uri":"http://www.eionet.europa.eu/gemet/concept/4358"},{"value":"predator","uri":"http://www.eionet.europa.eu/gemet/concept/6577"}],"keywordsTheme":[{"value":"Biodiversity","uri":"http://onto.nerc.ac.uk/CEHMD/topic/2"},{"value":"Agriculture","uri":"http://onto.nerc.ac.uk/CEHMD/topic/1"},{"value":"Ecosystem services","uri":"http://onto.nerc.ac.uk/CEHMD/topic/6"}],"licences":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}],"lineage":"Barley plants (Hordeum vulgare, cultivars Irina (KWS seeds) and Firefoxx (Elsoms seeds)), were treated with Acidovorax radicis N35, Bacillus subtilis B171, or a control, and either infested with Sitobion avenae aphids or left uninfested. \nIn the first experiment,  whole leaves were sampled, metabolites extracted with hexane, derivatised and analysed using an Agilent 7250 GC-qToF-MS equipped with an HP-5MS column (30 m × 0.25 mm × 0.25 μm). \nIn the second experiment, plants were enclosed in transparent PET film bags (31 cm × 30 cm; Toppits® Bratschlauch, Melitta Group, Germany) sealed at both ends and headspace volatiles were collected using Tenax probes and analysed on a GC-qToF-MS coupled with thermal desorption. Data were processed using MS-DIAL, with blank subtraction, internal standard normalisation, QC correction, and log transformation. Annotated peaks were matched against spectral libraries. \nFeatures were assigned metabolite numbers to enable unbiased comparisons, including unknowns, across both experiments.","metadataDate":"2026-06-27T19:32:36","notGEMINI":true,"pointsOfContact":[{"displayName":"Sharon Zytynska","organisationName":"University of Manchester","organisationIdentifier":"https://ror.org/027m9bs27","role":"pointOfContact","email":"sharon.zytynska@manchester.ac.uk","fullName":"Sharon Zytynska","pointOfContact":"University of Manchester"}],"publicationDate":"2025-11-13T00:00:00.000Z","publishers":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"}],"resourceIdentifiers":[{"code":"https://catalogue.ceh.ac.uk/id/e65881be-9dca-4111-8a3b-fb84cf78cf15"},{"code":"10.5285/e65881be-9dca-4111-8a3b-fb84cf78cf15","codeSpace":"doi"}],"resourceType":{"value":"nonGeographicDataset"},"responsibleParties":[{"displayName":"Sharon Zytynska","organisationName":"University of Manchester","organisationIdentifier":"https://ror.org/027m9bs27","role":"pointOfContact","email":"sharon.zytynska@manchester.ac.uk","fullName":"Sharon Zytynska","pointOfContact":"University of Manchester"},{"honorificPrefix":"Dr","familyName":"Parker","givenName":"Megan E.","organisationName":"University of Liverpool","organisationIdentifier":"https://ror.org/04xs57h96","role":"author","email":"m.e.parker@liverpool.ac.uk","nameIdentifier":"https://orcid.org/0009-0007-4602-8382","fullName":"Parker, M.E."},{"honorificPrefix":"Dr","familyName":"Zytynska","givenName":"Sharon E.","organisationName":"University of Manchester","organisationIdentifier":"https://ror.org/027m9bs27","role":"author","email":"sharon.zytynska@manchester.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-0174-3303","fullName":"Zytynska, S.E."},{"organisationName":"University of Liverpool","organisationIdentifier":"https://ror.org/04xs57h96","role":"rightsHolder"},{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"},{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"}],"rightsHolders":[{"organisationName":"University of Liverpool","organisationIdentifier":"https://ror.org/04xs57h96","role":"rightsHolder"}],"title":"Metabolomic data from the whole-leaf and headspace of barley plants inoculated with rhizobacteria and infested with aphids","topicCategories":[{"value":"biota","uri":"http://inspire.ec.europa.eu/metadata-codelist/TopicCategory/biota"}],"topics":["http://onto.nerc.ac.uk/CEHMD/topic/2","http://onto.nerc.ac.uk/CEHMD/topic/1","http://onto.nerc.ac.uk/CEHMD/topic/6"],"type":"nonGeographicDataset","uri":"https://catalogue.ceh.ac.uk/id/e65881be-9dca-4111-8a3b-fb84cf78cf15","useConstraints":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}]}