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This data was derived from DNA meta-barcoding of pollen extracted from honey samples provided by bee keepers archived as part of the UK National Honey Monitoring Scheme (https://honey-monitoring.ac.uk/). All data provided is from the first full year of the scheme in 2019. \n\nWorking in partnership with UK beekeepers, the National Honey Monitoring Scheme aims to use honeybees to monitor long-term changes in the condition and health of the UK countryside. Data associated with subsequent years will be made available as samples are processed.\n\nThe Honey Monitoring Scheme is supported by national capability funding from UK Centre for Ecology & Hydrology under the ASSIST programme. ","distributionFormats":[{"name":"Comma-separated values (CSV)","type":"text/csv","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"funding":[{"funderName":"Natural Environment Research Council","funderIdentifier":"https://ror.org/02b5d8509","awardTitle":"Achieving Sustainable Agricultural Systems (ASSIST)","awardNumber":"NE/N018125/1","awardURI":"https://gtr.ukri.org/projects?ref=NE/N018125/1","orcid":false,"ror":true}],"id":"e9ec63be-3f2b-4d1b-b9bf-77ca2b96c7f5","incomingCitationCount":0,"infoLinks":[{"url":"https://data-package.ceh.ac.uk/sd/e9ec63be-3f2b-4d1b-b9bf-77ca2b96c7f5.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"inspireThemes":[{"theme":"Environmental Monitoring Facilities","uri":"http://inspire.ec.europa.eu/theme/ef"}],"keywordsOther":[{"value":"Honey"},{"value":"DNA Metabarcoding"},{"value":"Forage plants"},{"value":"National Honey Monitoring Scheme"},{"value":"Assist"},{"value":"Apis mellifera","uri":"http://www.wikidata.org/entity/Q30034"},{"value":"Honeybee","uri":"http://www.wikidata.org/entity/Q30034"},{"value":"pollen","uri":"http://www.eionet.europa.eu/gemet/concept/6392"},{"value":"forage crop","uri":"http://www.eionet.europa.eu/gemet/concept/11177"}],"keywordsPlace":[{"value":"United Kingdom"}],"keywordsTheme":[{"value":"Agriculture","uri":"http://onto.nerc.ac.uk/CEHMD/topic/1"},{"value":"Biodiversity","uri":"http://onto.nerc.ac.uk/CEHMD/topic/2"},{"value":"Pollinators","uri":"http://onto.nerc.ac.uk/CEHMD/topic/14"}],"licences":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}],"lineage":"All honey samples were submitted following an application though the National Honey Monitoring Scheme online portal which required minimum meta-data on site location and sample date which was verified.   This included in some cases additional data on hive health metrics.  DNA meta-barcoding to rapidly process samples to identify plant species.  Quality assurance of the DNA metabarcoding was delivered through operational deployment of sophisticated protocols for barcoding and interpreting large volumes of honey samples. To do this we developed the HONEYPI pipeline implemented in python 2.7 and is open access (https://github.com/hsgweon/honeypi).  The HONEYPI pipeline is divided into several parts as follows: 1) the raw amplicon sequences are quality filtered and adapters removed; 2) DADA2 pipeline is subsequently used to generate an Amplicon Sequence Variant (ASV) abundance table containing chimera-removed, high-quality error-corrected sequences. 3). For each ASV, conserved regions flanking ITS2 are removed; and (4) resulting sequences taxonomically classified using the naive Bayesian classifier against in-house ITS2 database.  Since HONEYPI uses ASVs rather than clusters of sequences for classification, it allows combining of ASV tables, i.e. data from two or more separate sequencing runs can be merged without re-clustering sequences. \n\nA full open access methodological paper describing this approach is given in Oliver et al (2021) MethodsX, 8, 101303 (https://doi.org/10.1016/j.mex.2021.101303)","metadataDate":"2026-07-03T08:42:08","notGEMINI":false,"onlineResources":[{"url":"https://data-package.ceh.ac.uk/data/e9ec63be-3f2b-4d1b-b9bf-77ca2b96c7f5","name":"Download the data","description":"Download a copy of this data","function":"download","type":"OTHER"},{"url":"https://data-package.ceh.ac.uk/sd/e9ec63be-3f2b-4d1b-b9bf-77ca2b96c7f5.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"pointsOfContact":[{"familyName":"Woodcock","givenName":"B.A. ","organisationName":"UK Centre for Ecology & Hydrology","organisationIdentifier":"https://ror.org/00pggkr55","role":"pointOfContact","email":"enquiries@ceh.ac.uk","nameIdentifier":"https://orcid.org/0000-0003-0300-9951","fullName":"Woodcock, B.A.","pointOfContact":"UK Centre for Ecology & Hydrology"}],"publicationDate":"2022-01-18T00:00:00.000Z","publishers":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"}],"resourceIdentifiers":[{"code":"https://catalogue.ceh.ac.uk/id/e9ec63be-3f2b-4d1b-b9bf-77ca2b96c7f5"},{"code":"10.5285/e9ec63be-3f2b-4d1b-b9bf-77ca2b96c7f5","codeSpace":"doi"}],"resourceMaintenance":[{"frequencyOfUpdate":"asNeeded"}],"resourceType":{"value":"dataset"},"responsibleParties":[{"familyName":"Woodcock","givenName":"B.A. ","organisationName":"UK Centre for Ecology & Hydrology","organisationIdentifier":"https://ror.org/00pggkr55","role":"author","email":"enquiries@ceh.ac.uk","nameIdentifier":"https://orcid.org/0000-0003-0300-9951","fullName":"Woodcock, B.A."},{"familyName":"Oliver","givenName":"A.E.","organisationName":"UK Centre for Ecology & Hydrology","organisationIdentifier":"https://ror.org/00pggkr55","role":"author","email":"enquiries@ceh.ac.uk","nameIdentifier":"https://orcid.org/0000-0003-4923-277X","fullName":"Oliver, A.E."},{"familyName":"Newbold","givenName":"L.K.","organisationName":"UK Centre for Ecology & Hydrology","organisationIdentifier":"https://ror.org/00pggkr55","role":"author","email":"enquiries@ceh.ac.uk","nameIdentifier":"https://orcid.org/0000-0001-8895-1406","fullName":"Newbold, L.K."},{"familyName":"Gweon","givenName":"H.S.","organisationName":"University of Reading","organisationIdentifier":"https://ror.org/05v62cm79","role":"author","email":"h.s.gweon@reading.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-6218-6301","fullName":"Gweon, H.S."},{"familyName":"Roy","givenName":"D.B.","organisationName":"UK Centre for Ecology & Hydrology","organisationIdentifier":"https://ror.org/00pggkr55","role":"author","email":"enquiries@ceh.ac.uk","nameIdentifier":"https://orcid.org/0000-0002-5147-0331","fullName":"Roy, D.B."},{"familyName":"Pywell","givenName":"R.F.","organisationName":"UK Centre for Ecology & Hydrology","organisationIdentifier":"https://ror.org/00pggkr55","role":"author","email":"enquiries@ceh.ac.uk","nameIdentifier":"https://orcid.org/0000-0001-6431-9959","fullName":"Pywell, R.F."},{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"},{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"custodian","email":"info@eidc.ac.uk"},{"organisationName":"UK Centre for Ecology & Hydrology","organisationIdentifier":"https://ror.org/00pggkr55","role":"rightsHolder","email":"enquiries@ceh.ac.uk"},{"familyName":"Woodcock","givenName":"B.A. ","organisationName":"UK Centre for Ecology & Hydrology","organisationIdentifier":"https://ror.org/00pggkr55","role":"pointOfContact","email":"enquiries@ceh.ac.uk","nameIdentifier":"https://orcid.org/0000-0003-0300-9951","fullName":"Woodcock, B.A.","pointOfContact":"UK Centre for Ecology & Hydrology"}],"rightsHolders":[{"organisationName":"UK Centre for Ecology & Hydrology","organisationIdentifier":"https://ror.org/00pggkr55","role":"rightsHolder","email":"enquiries@ceh.ac.uk"}],"spatialReferenceSystems":[{"code":"unknown","title":"UK regions"}],"spatialRepresentationTypes":["textTable"],"supplemental":[{"name":"National Honey Monitoring Scheme website","url":"https://honey-monitoring.ac.uk/","function":"website"},{"description":"Oliver, A. E., Newbold, L. K., Gweon, H. S., Read, D. S., Woodcock, B. A., & Pywell, R. F. (2021). Integration of DNA extraction, metabarcoding and an informatics pipeline to underpin a national citizen science honey monitoring scheme. In MethodsX (Vol. 8, p. 101303). Elsevier BV","url":"https://doi.org/10.1016/j.mex.2021.101303","function":"relatedArticle"}],"temporalExtents":[{"begin":"2019-01-01","end":"2019-12-31"}],"title":"Data describing pollen identified from honey samples originating from the UKCEH National Honey Monitoring Scheme for 2019","topicCategories":[{"value":"biota","uri":"http://inspire.ec.europa.eu/metadata-codelist/TopicCategory/biota"}],"topics":["http://onto.nerc.ac.uk/CEHMD/topic/1","http://onto.nerc.ac.uk/CEHMD/topic/2","http://onto.nerc.ac.uk/CEHMD/topic/14"],"type":"dataset","uri":"https://catalogue.ceh.ac.uk/id/e9ec63be-3f2b-4d1b-b9bf-77ca2b96c7f5","useConstraints":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}]}