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Each well in the plate had 1 bead composed of polycaprolactone in it and 150 microlitres of  Isopropyl β- d-1-thiogalactopyranoside (IPTG) induced bacterial cell culture. Three replicates were performed. The bacterial cells had been modified to harbour plasmids that encoded genes to trigger the cells to form biofilms, the expression of these genes was induced using IPTG. An empty plasmid was used as the control. \nThe work was supported by the Natural Environment Research Council (Grant NE/X010902/1), as part of an experiment looking into using synthetic biology to manipulate bacterial social behaviours to maximise the microbial degradation of environmental waste plastics.","distributionFormats":[{"name":"Comma-separated values (CSV)","type":"text/csv","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"funding":[{"funderName":"Natural Environment Research Council","funderIdentifier":"https://ror.org/02b5d8509","awardNumber":"NE/X010902/1","awardURI":"https://gtr.ukri.org/projects?ref=NE/X010902/1","orcid":false,"ror":true}],"id":"eef3a20c-cb4c-415f-b4be-4d1c31fb6015","incomingCitationCount":0,"infoLinks":[{"url":"https://data-package.ceh.ac.uk/sd/eef3a20c-cb4c-415f-b4be-4d1c31fb6015.zip","name":"Supporting information","description":"Supporting information available to assist in re-use of this dataset","function":"information","type":"OTHER"}],"inspireThemes":[{"theme":"Human Health and Safety","uri":"http://inspire.ec.europa.eu/theme/hh"}],"keywordsOther":[{"value":"Biofilm"},{"value":"plastic degradation"},{"value":"polyesterase"},{"value":"Escherichia coli"}],"keywordsTheme":[{"value":"Pollution","uri":"http://onto.nerc.ac.uk/CEHMD/topic/15"}],"licences":[{"value":"This resource is licensed under a Creative Commons Attribution 4.0 International Licence","code":"license","uri":"https://eidc.ac.uk/licences/cc-by/plain"}],"lineage":"Controls included bacteria with empty vectors that did not contain the genes required to induce biofilm formation. Colonies were recovered from plastic beads following incubation for 24 hours at 37 degrees Celsius with bacteria engineered to have higher levels of biofilm formation. Beads were subsequently removed, washed 3 times in PBS and subsequently sonicated for 10 minutes to remove bacterial biofilm. Serial dilutions were made from the PBS suspension and spotted in triplicate on Luria Broth (LB) agar containing appropriate antibiotics. Plates were incubated for 16 hours at 37 degrees Celsius before colonies were counted. 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