PREFIX rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#>
PREFIX rdfs: <http://www.w3.org/2000/01/rdf-schema#>
PREFIX skos: <http://www.w3.org/2004/02/skos/core#>
PREFIX owl: <http://www.w3.org/2002/07/owl#>
PREFIX xsd: <http://www.w3.org/2001/XMLSchema#>
PREFIX dcterms: <http://purl.org/dc/terms/>
PREFIX dcat: <http://www.w3.org/ns/dcat#>
PREFIX dcmitype: <http://purl.org/dc/dcmitype/>
PREFIX foaf: <http://xmlns.com/foaf/0.1/>
PREFIX geo: <http://www.opengis.net/ont/geosparql#>
PREFIX vcard: <http://www.w3.org/2006/vcard/ns#>
PREFIX prov: <http://www.w3.org/ns/prov#>
PREFIX odrs: <http://schema.theodi.org/odrs#>
PREFIX sosa: <http://www.w3.org/ns/sosa/>
PREFIX sdo: <https://schema.org/>
PREFIX ef: <http://www.w3.org/2015/03/inspire/ef/>
PREFIX doo: <https://digital.ceh.ac.uk/ontology/doo/>
PREFIX adms: <http://www.w3.org/ns/adms#>
PREFIX : <https://catalogue.ceh.ac.uk/id/>
:eef3a20c-cb4c-415f-b4be-4d1c31fb6015
dcterms:title "Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions" ;
dcterms:identifier "https://catalogue.ceh.ac.uk/id/eef3a20c-cb4c-415f-b4be-4d1c31fb6015","https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015" ;
dcterms:bibliographicCitation "Howard, S., McCarthy, R.R. (2024). Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015" ;
dcterms:description "This dataset contains information about the number of bacterial colonies recovered from plastics beads that were incubated in a 96-well plate for 24 hours at 37 degrees Celsius. Each well in the plate had 1 bead composed of polycaprolactone in it and 150 microlitres of Isopropyl β- d-1-thiogalactopyranoside (IPTG) induced bacterial cell culture. Three replicates were performed. The bacterial cells had been modified to harbour plasmids that encoded genes to trigger the cells to form biofilms, the expression of these genes was induced using IPTG. An empty plasmid was used as the control. The work was supported by the Natural Environment Research Council (Grant NE/X010902/1), as part of an experiment looking into using synthetic biology to manipulate bacterial social behaviours to maximise the microbial degradation of environmental waste plastics." ;
dcterms:provenance [
a dcterms:ProvenanceStatement ;
rdfs:label "Controls included bacteria with empty vectors that did not contain the genes required to induce biofilm formation. Colonies were recovered from plastic beads following incubation for 24 hours at 37 degrees Celsius with bacteria engineered to have higher levels of biofilm formation. Beads were subsequently removed, washed 3 times in PBS and subsequently sonicated for 10 minutes to remove bacterial biofilm. Serial dilutions were made from the PBS suspension and spotted in triplicate on Luria Broth (LB) agar containing appropriate antibiotics. Plates were incubated for 16 hours at 37 degrees Celsius before colonies were counted."
] ;
dcat:contactPoint
:eef3a20c-cb4c-415f-b4be-4d1c31fb6015_c0 ;
dcterms:publisher
<https://ror.org/04xw4m193> ;
dcterms:subject
<http://onto.nerc.ac.uk/CEHMD/topic/15>,
"Biofilm",
"plastic degradation",
"polyesterase",
"Escherichia coli" ;
prov:wasGeneratedBy
<https://gtr.ukri.org/projects?ref=NE/X010902/1> ;
a dcat:Dataset;
dcterms:type dcmitype:Dataset ;
dcterms:available "2024-10-28"^^xsd:date ;
dcat:landingPage <https://catalogue.ceh.ac.uk/id/eef3a20c-cb4c-415f-b4be-4d1c31fb6015>, <https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015> ;
dcterms:license <https://eidc.ac.uk/licences/cc-by/plain>
;
dcterms:accessRights <http://vocab.nerc.ac.uk/collection/N07/current/UNRS/> ;
dcterms:theme
<http://onto.nerc.ac.uk/CEHMD/topic/15> ;
dcat:distribution [
dcat:accessURL
<https://data-package.ceh.ac.uk/data/eef3a20c-cb4c-415f-b4be-4d1c31fb6015> ;
dcterms:license <https://eidc.ac.uk/licences/cc-by/plain>
;
dcterms:accessRights <http://vocab.nerc.ac.uk/collection/N07/current/UNRS/> ;
dcterms:format
[
a dcterms:IMT ;
rdf:value "Comma-separated values (CSV)" ; rdfs:label "Comma-separated values (CSV)"
] ;
];
dcterms:creator
:eef3a20c-cb4c-415f-b4be-4d1c31fb6015_a0,
:eef3a20c-cb4c-415f-b4be-4d1c31fb6015_a1 ;
dcterms:language <http://id.loc.gov/vocabulary/iso639-1/en> .
:eef3a20c-cb4c-415f-b4be-4d1c31fb6015_c0 a foaf:Person ;
foaf:name "McCarthy, R.R." ;
foaf:familyName "McCarthy" ;
foaf:givenName "R.R." ;
vcard:hasEmail "Ronan.mccarthy@brunel.ac.uk" ;
foaf:member <https://ror.org/00dn4t376> ;
.
<https://ror.org/04xw4m193> a foaf:Organization ;
foaf:name "NERC EDS Environmental Information Data Centre" ;
vcard:hasEmail "info@eidc.ac.uk" ;
foaf:member <https://ror.org/04xw4m193> ;
.
:eef3a20c-cb4c-415f-b4be-4d1c31fb6015_a0 a foaf:Person ;
foaf:name "Howard, S." ;
foaf:familyName "Howard" ;
foaf:givenName "S." ;
vcard:hasEmail "Sophie.howard@brunel.ac.uk" ;
foaf:member <https://ror.org/00dn4t376> ;
.
:eef3a20c-cb4c-415f-b4be-4d1c31fb6015_a1 a foaf:Person ;
foaf:name "McCarthy, R.R." ;
foaf:familyName "McCarthy" ;
foaf:givenName "R.R." ;
vcard:hasEmail "Ronan.mccarthy@brunel.ac.uk" ;
foaf:member <https://ror.org/00dn4t376> ;
.
<http://onto.nerc.ac.uk/CEHMD/topic/15> a skos:Concept;
skos:prefLabel "Pollution"; rdfs:label "Pollution"
.
<https://ror.org/00dn4t376> a foaf:Organization ;
foaf:name "Brunel University London" .
<https://gtr.ukri.org/projects?ref=NE/X010902/1> a prov:Activity ; .