Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform
bibliographic citation: Hodgson, C.J. (2020). Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre. https://doi.org/10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce
description: The data consists of the abundance of antibiotic resistant genes (ARG) in fresh cattle faeces from faecal samples taken from a cohort of 30 cattle. The cattle were from the North Wyke Farm Platform, a UK national capability, located at Rothamsted Research, North Wyke in Devon. Faecal samples were collected in November 2016. The cohort of 30 cattle were selected from 90 animals, ten from each of the three farmlets. Samples were collected from cattle as they entered housing for the winter, i.e. at weaning.
provenance: ProvenanceStatement
  label: Faecal samples were taken by members of staff from Rothamsted Research North Wyke. In the laboratory faecal samples were pooled, such that for each farmlet (10 cattle) there were 3 samples, (3, 3, 4 cattle). This resulted in 9 faecal samples from which DNA was extracted using a commercially available extraction kit (MO BIO PowerSoil® DNA Isolation kit (catalogue No. 12888-100). The following adaption to the Power Soil protocol; at sample preparation samples were heated to 65 °C for 10 minutes prior to vortex. DNA was sequenced by the sequencing unit at the University of Exeter. The resulting metagenomic data was analysed at the Environmental Biotechnology Laboratory at the University of Hong Kong. The establishment licence under which the experiment was conducted was XA11784A2 and the project licence was P592D2677. Data was collected for the NERC funded “Does the potential for AMR selection differ between common UK cattle grazing systems?” NE/N019792/1.
spatial: Location
temporal: PeriodOfTime
  start date: 2016-11-01
  end date: 2016-11-30
publisher: NERC Environmental Information Data Centre
subject: Antibiotic resistant gene abundance
subject: North Wyke Farm Platform
was generated by: https://gtr.ukri.org/projects
type: Dataset
available: 2020-05-18
landing page: https://doi.org/10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce
license: OGL-UK-3.0.ttl
rights: RightsStatement
  copyright notice: copyright Natural Environment Research Council
access rights: UNRS
theme: Antibiotic resistant gene abundance
distribution:
  access URL: https://data-package.ceh.ac.uk/data/efcef5e3-09f2-4a23-ad19-53295a3171ce
  license: OGL-UK-3.0.ttl
  rights: RightsStatement
    copyright notice: copyright Natural Environment Research Council
  access rights: UNRS
  format: Comma-separated values (CSV)
creator: Hodgson, C.J.
language: en

Hodgson, C.
type: Person
family name: Hodgson
given name: Chris
member: Rothamsted Research North Wyke

NERC Environmental Information Data Centre
type: Organization

Hodgson, C.J.
type: Person
family name: Hodgson
given name: C.J.
member: Rothamsted Research North Wyke

Rothamsted Research North Wyke
type: Organization