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Each of the plots was further divided into three grids (grassland grids 1 to 3 / woodland grids 4 to 6) with grids 1 and 6 being the furthest apart. The contrast boundary is between grids 3 and 4. In each plot, nine sampling locations were randomly sampled (three in each grid). The five grassland-to-woodland contrasts were located across England: Gisburn (Gisburn-1, Gisburn-2), Alice Holt, Wytham Woods, and Kielder Forest. \n\nCommon soil physical and chemical properties were measured; field water content, soil water content, electrical conductivity, bulk density, pH in DIW, pH in CaCl2, Loss-on-ignition, total soil carbon, total soil nitrogen, and total soil phosphorus. Less common properties were also measured; extractable soil nitrogen, dissolved organic carbon, soil aggregate sizes, and soil texture. And for specific soil cores these additional following properties were measured; soil density fractions and their carbon and nitrogen contents, cation exchange capacity, Sodium concentration, potassium concentration, calcium concentration, magnesium concentration, and extracellular enzyme activities. Derived metrics resulting from the bioinformatic processing of the raw sequence files are provided as part this dataset.\n\nThe properties in this dataset are co-located with ANPP estimates, litter layer depth measurements, soil hydraulic measurements (soil water release curves and hydraulic conductivity), earthworm counts and identification. A file   is provided to connect all co-located measurements.\n","distributionFormats":[{"name":"Comma-separated values (CSV)","type":"text/csv","version":"unknown"}],"distributorContacts":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"distributor","email":"info@eidc.ac.uk"}],"funding":[{"funderName":"Natural Environment Research Council","funderIdentifier":"https://ror.org/02b5d8509","awardNumber":"NE/R016429/1","awardURI":"https://gtr.ukri.org/projects?ref=NE/R016429/1","orcid":false,"ror":true}],"id":"f127b12d-a54f-4ab8-9697-b8154b81c4bd","incomingCitationCount":0,"inspireThemes":[{"theme":"Soil","uri":"http://inspire.ec.europa.eu/theme/so"}],"keywordsOther":[{"value":"SOC"},{"value":"carbon","uri":"http://onto.nerc.ac.uk/CAST/270"},{"value":"woodland"},{"value":"topsoil"},{"value":"soil physics"},{"value":"microbial genes"},{"value":"meta genome"},{"value":"co-located measurements"},{"value":"SOC-D"},{"value":"UK-SCAPE"},{"value":"soil biology","uri":"http://www.eionet.europa.eu/gemet/concept/7847"},{"value":"soil chemistry","uri":"http://www.eionet.europa.eu/gemet/concept/7849"},{"value":"soil","uri":"http://www.eionet.europa.eu/gemet/concept/7843"},{"value":"subsoil","uri":"http://www.eionet.europa.eu/gemet/concept/8167"},{"value":"grassland","uri":"http://www.eionet.europa.eu/gemet/concept/3733"}],"keywordsTheme":[{"value":"Soil","uri":"http://onto.nerc.ac.uk/CEHMD/topic/17"}],"licences":[{"value":"This resource is available under the terms of the Open Government Licence","code":"license","uri":"https://eidc.ac.uk/licences/ogl/plain"}],"lineage":"In each plot, nine sampling locations were randomly sampled (three in each grid). At each sampling location 18, 100 cm soil cores were taken.\n\nFor all the soil cores, the following common soil physical and chemical properties were measured in up to six depth increments: field water content, soil water content, electrical conductivity, bulk density, pH in DIW, pH in CaCl2, Loss-on-ignition, total soil carbon, total soil nitrogen, total soil phosphorus. Also, the following less common chemical, physical and biological properties were measured in one topsoil and one subsoil increment; extractable soil nitrogen, dissolved organic carbon, soil aggregate sizes, soil texture.\n\nFor the soil cores from grids 2 and 5 the following additional soil properties were measured: soil density fractions and their carbon and nitrogen contents, cation exchange capacity, sodium concentration, potassium concentration, calcium concentration, magnesium concentration, extracellular enzyme activities.\n\nThe raw sequence files from the microbial analyses of bacterial and fungal marker genes (16S rRNA gene, and ITS regions respectively) and shotgun whole genome sequencing have been deposited in the European Nucleotide Archive (ENA) under project accession PRJEB66294 (https://www.ebi.ac.uk/ena/browser/view/PRJEB66294).\n\nAll the physical, chemical, and biological properties were measured using standard techniques, see the supporting documentation for more details.   \n","metadataDate":"2026-06-29T15:55:35","notGEMINI":false,"pointsOfContact":[{"familyName":"Reinsch","givenName":"S.","organisationName":"UK Centre for Ecology & Hydrology","organisationIdentifier":"https://ror.org/00pggkr55","role":"pointOfContact","email":"enquiries@ceh.ac.uk","address":{"deliveryPoint":"Environment Centre Wales, Deiniol Road","city":"Bangor","administrativeArea":"Gwynedd","postalCode":"LL57 2UW","country":"United Kingdom"},"fullName":"Reinsch, S.","pointOfContact":"UK Centre for Ecology & Hydrology"}],"publicationDate":"2023-10-27T00:00:00.000Z","publishers":[{"organisationName":"NERC EDS Environmental Information Data Centre","organisationIdentifier":"https://ror.org/04xw4m193","role":"publisher","email":"info@eidc.ac.uk"}],"reasonChanged":"The author list in this version was incorrect, the new version has a correct author list - the data has not 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