Leitão, A.B.; Ramesh, A.; Day, J.P.; Hanna, N.; Devi, A.; Hayes, M.P.; Jiggins, F.M.

Changes in gene expression and encapsulation in Drosophila melanogaster caused by exposure to parasitoid wasps v2

https://doi.org/10.5285/06ea87f3-476d-40fd-acce-e6923e786d48 Cite this dataset
This is the most recent version of this dataset (view other versions )
Data consists of gene expression estimates and encapsulation rates in wild caught Drosophila melanogaster larvae following exposure to different treatments. Treatments include injection with wasp homogenate, injection with oil and no injection. Also provided are functional enrichment categories for differentially expressed genes and library generation and read mapping metrics. The data were produced under the grant: NE/P00184X/1 Why does Drosophila vary in susceptibility to parasitoid wasps.
Publication date: 2022-03-08

Formats

Comma-separated values (CSV)
txt

Where/When

Study area
Temporal extent
2017-10-01    to    2017-10-31

Provenance & quality

Drosophila melanogaster larvae obtained from a wild caught population were exposed injected with wasp homogenate, oil or no manipulation was made. Encapsulation rates were measured in each case in the fly larvae. For RNA-sequencing, hemocyte and fat body tissues were isolated from third instar larvae 24 hours post experimental manipulation. RNA was isolated for sequencing. Sequenced reads were trimmed, mapped to the dm6 genome, number of reads mapping to each gene was counted, differential expression tests were applied, and functional enrichment was done.

Correspondence/contact details

Ramesh, A.
University of Cambridge
UNITED KINGDOM
 ar885@cam.ac.uk

Authors

Leitão, A.B.
University of Cambridge
ORCID iD icon https://orcid.org/0000-0001-9004-1831
Ramesh, A.
University of Cambridge
ORCID iD icon https://orcid.org/0000-0003-0050-3516
Day, J.P.
University of Cambridge
Hanna, N.
University of Cambridge
Devi, A.
University of Cambridge
Hayes, M.P.
University of Cambridge
Jiggins, F.M.
University of Cambridge
ORCID iD icon https://orcid.org/0000-0001-7470-8157

Other contacts

Rights holder
University of Cambridge
Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
Biota
Keywords
differential gene expression,  Drosophila melanogaster,  encapsulation,  Evolutionary genetics,  fat body,  hemocyte,  insect genomics,  Leptopilina boulardi,  parasitoid wasp,  RNA-sequencing
INSPIRE Theme
Habitats and Biotopes
Funding
Natural Environment Research Council Award: NE/P00184X/1
Spatial representation type
Tabular (text)
Spatial reference system
OSGB 1936 / British National Grid
Last updated
18 May 2022 12:21

Get the data

By accessing or using this dataset, you agree to the terms of the relevant licence agreement(s). You will ensure that this dataset is cited in any publication that describes research in which the data have been used.

This dataset is available under the terms of the Open Government Licence

Download the data Supporting documentation

CITE AS: Leitão, A.B.; Ramesh, A.; Day, J.P.; Hanna, N.; Devi, A.; Hayes, M.P.; Jiggins, F.M. (2022). Changes in gene expression and encapsulation in Drosophila melanogaster caused by exposure to parasitoid wasps v2. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/06ea87f3-476d-40fd-acce-e6923e786d48