Griffiths, R.; Edwards, F.; Scarlett, P.; Webb, G.; Sarlej, R.; Thomson, B.

Operational taxonomic units for epilithon bacteria in the rivers Wolf and Tamar catchments

Name: Operational taxonomic units for epilithon bacteria in the rivers Wolf and Tamar catchments
Published: 2014-05-14
License: https://eidc.ceh.ac.uk/licences/OGL/plain

https://doi.org/10.5285/16649ff0-af24-41b0-bcb4-15e610dac170 Cite this dataset

This dataset contains operational taxonomic units for epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

Publication date: 2014-05-14

Format

Comma-separated values (CSV)

Spatial information

Study area
Spatial representation type: Tabular (text)
Spatial reference system: OSGB 1936 / British National Grid

Temporal information

Temporal extent: 2013-10-01 to 2014-03-20

Provenance & quality

Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

This dataset is included in the following collections

Molecular analyses of soils and epilithon samples from the Tamar catchment

Correspondence/contact details

Griffiths, R.

UK Centre for Ecology & Hydrology

Maclean Building, Benson Lane, Crowmarsh Gifford
Wallingford
Oxfordshire
OX10 8BB
UNITED KINGDOM

enquiries@ceh.ac.uk