Goodwin, M. et al

Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016)

The data set includes the results of a laboratory analysis in 2016, investigating the presence of trypanosomes and prevalence of tsetse endosymbionts in tsetse flies. The tsetse flies were sampled in Hurungwe District, Mashonaland West Province, Zimbabwe, from February 2014 to November 2014. Flies were sampled using a combination of Epsilon traps and fly rounds, both established techniques for sampling tsetse. Tsetse were stored prior to laboratory analysis using Polymerase chain reaction (PCR) techniques in 2016. The data include two species of tsetse, Glossina pallidipes and Glossina morsitans morsitans. Trypanosome species investigated include Trypanosoma brucei s.l., T. b. rhodesiense, T. vivax, T. congolense, T. simiae, T.simiae (Tsavo) and T. godfreyi. Endosymbionts included in the study were Sodalis glossinidius and Wolbachia spp. Hurungwe District is the only sleeping sickness focus in Zimbabwe and an increase in cases had been detected in years preceding this study. The objective of the study was to investigate the trypanosome species present in the tsetse population and their association with tsetse endosymbionts. This study was conducted as part of research into the relationship between trypanosomiasis, well-being and ecosystems by the Dynamic Drivers of Disease in Africa Consortium (DDDAC). The research was funded by NERC with support from the Ecosystem Services for Poverty Alleviation Programme (ESPA).

Publication date: 2017-04-21

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Format of the dataset : Comma-separated values (CSV)

Access and use conditions

This resource is embargoed and will be made available by 1 December 2018 at the latest  

This resource will be available under the terms of the Open Government Licence

You must cite: Goodwin, M.; Webber, F.; Hamill, L.C.; Anderson, N.E.; Shereni, W.; Nyakupinda, L.; Gwenhure, L.F.; Macleod, E. (2017). Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016). NERC Environmental Information Data Centre. https://doi.org/10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c

 

© The University of Edinburgh

© Central Veterinary Research and Diagnostic Laboratory, Zimbabwe

© Department of Livestock and Veterinary Services, Zimbabwe

This dataset is part of the following

Where/When

Study area
Temporal extent
2014-02-01    to    2016-04-12

Provenance & quality

The data presents the molecular analysis of 101 Glossina morsitans morsitans and 108 Glossina pallidipes tsetse sampled in Hurungwe District, Mashonaland West Province, Zimbabwe. Tsetse were sampled using Epsilon traps and fly rounds as described by Shereni et al. (2016). Tsetse were stored in cryotubes with desiccant beads at room temperature. DNA was extracted from the tsetse using Qiagen DNeasy kits and then subjected to polymerase chain reactions (PCR) to detect the primary endosymbiont, Wigglesworthia glossindia. Samples from which no DNA was amplified were discounted from further molecular analysis. All remaining samples were subjected to PCR reactions to detect African trypanosomes and the secondary tsetse symbionts, Sodalis glossinidius and Wolbachia. All PCRs included a negative and a positive control. The data were collated in an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC.

Correspondence/contact details

Anderson, N.E.
The University of Edinburgh
The Royal (Dick) School of Veterinary Studies and The Roslin Institute Easter Bush Campus
Edinburgh
Midlothian
EH25 9RG
United Kingdom
Neil.Anderson@ed.ac.uk

Authors

Goodwin, M.
The University of Edinburgh
Webber, F.
The University of Edinburgh
Hamill, L.C.
The University of Edinburgh
Anderson, N.E.
The University of Edinburgh
Shereni, W.
Department of Livestock and Veterinary Services, Zimbabwe
Nyakupinda, L.
Department of Livestock and Veterinary Services, Zimbabwe
Gwenhure, L.F.
Central Veterinary Research and Diagnostic Laboratory, Zimbabwe
Macleod, E.
The University of Edinburgh

Other contacts

Custodian
Environmental Information Data Centre
eidc@ceh.ac.uk
Publisher
NERC Environmental Information Data Centre
eidc@ceh.ac.uk

Spatial

Spatial representation type
Tabular (text)

Tags

Topic categories
Biota , Health
Keywords
Dynamic Drivers of Disease in Africa Consortium (DDDAC),  Glossina morsitans morsitans Glossina pallidipes Hurungwe District Sleeping sickness,  Sodalis glossinidius Trypanosoma brucei Trypanosoma congolense Trypanosoma godfreyi,  Trypanosoma simiae Trypanosoma vivax Wolbachia spp Zimbabwe