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Ormsby, M.J.; White, H.L.; Metcalf, R.; Oliver, D.M.; Quilliam, R.S.

Survival and persistence of clinically relevant E. coli on plastic waste under simulated environmental conditions

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This dataset is available under the terms of the Open Government Licence

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https://doi.org/10.5285/268cab8c-459c-4bf8-9b90-838c4d18efb2
This dataset contains information relating to the survival and persistence of clinically relevant strains of E. coli on plastic and cotton, under environmentally relevant conditions. The data encompass bacterial strain identity, material type, timepoints (days), bacterial concentration (CFU/ml) and survival percentages. Data were sampled over a 28-day period using culture-based approaches; and the ability of isolates to cause disease following their recovery was determined through the use of a Galleria mellonella model.

This work was supported by the UKRI Natural Environment Research Council (NERC) as part of the GCRF SPACES project [grant number NE/V005847/1] and the Plastic Vectors project, “Microbial hitch-hikers of marine plastics: the survival, persistence & ecology of microbial communities in the ‘Plastisphere’” [grant number NE/S005196/1].
Publication date: 2025-04-09
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More information

View numbers valid from 09 April 2025 Download numbers valid from 09 April 2025 (information prior to this was not collected)

Format

Comma-separated values (CSV)

Temporal information

Temporal extent
2023-01-01    to    2023-06-28

Provenance & quality

Biofilm formation, persistence and pathogenicity of E. coli were investigated through controlled laboratory experiments. Biofilms were generated on various materials in nutrient-rich and surface water environments, quantified using crystal violet staining, and analysed in triplicate. Persistence was assessed by recovering E. coli from biofilms over 28 days, followed by serial dilution, and selective plating.

Pathogenicity was evaluated using Galleria mellonella larvae, with standardised inoculation protocols and survival monitoring for 72 hours. Data underwent thorough validation at each stage, including replication, and selective media confirmation, ensuring high reliability and accuracy before submission.

Licensing and constraints

This dataset is available under the terms of the Open Government Licence

Cite this dataset as:
Ormsby, M.J.; White, H.L.; Metcalf, R.; Oliver, D.M.; Quilliam, R.S. (2025). Survival and persistence of clinically relevant E. coli on plastic waste under simulated environmental conditions. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/268cab8c-459c-4bf8-9b90-838c4d18efb2

Correspondence/contact details

Ormsby, M.J.
University of Stirling
 michael.ormsby1@stir.ac.uk

Authors

Ormsby, M.J.
University of Stirling
White, H.L.
University of Stirling
Metcalf, R.
University of Stirling
Oliver, D.M.
University of Stirling
Quilliam, R.S.
University of Stirling

Other contacts

Rights holder
University of Stirling
Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
environment
INSPIRE theme
Human Health and Safety
Keywords
biofilm , human pathogens , plastic waste , plastisphere , Pollution , public health , wastewater
Funding
Natural Environment Research Council Award: NE/V005847/1
Natural Environment Research Council Award: NE/S005196/1
Last updated
10 April 2025 15:32