The datasets generated by this project relate to the prevalence and abundance of antibiotic resistant bacteria and resistance genes in healthy adult humans, poultry and the outdoor environment in Bangladesh. Escherichia coli was used as a sentinel organism for antibiotic resistance. The aim of the project was to identify drivers of antibiotic resistance and pathways of transmission of resistance between the environment, animals and humans. The project ran from June 2016 to January 2019 and was carried out by researchers at Loughborough University in collaboration with the International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b). The project was funded by the Antimicrobial Resistance Cross Council Initiative supported by the seven research councils in partnership with the Department of Health and Department for Environment Food & Rural Affairs, NERC award number NE/N019555/1.