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Hewitt, S.; Warry, A.; Dyer, P.S.; Avery, S.

Heterogeneity and environmental stress resistance of wild yeast isolates

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This dataset is available under the terms of the Open Government Licence

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https://doi.org/10.5285/
This dataset contains analytical results for wild yeast isolates from soil samples at and near a disused metal smelting works in the north-east of the UK. The main contaminant from the smelting works was lead (Pb), but Cd was also elevated. The soil near the smelting works showed above-background levels of Pb. Yeasts of similar colony morphology on laboratory agar were isolated and proved to be mostly isolates of Saitozyma podzolica: 56 independent clonal isolates of this yeast were confirmed by ITS sequencing and RAPD-PCR. All of the S. podzolica isolates were tested for Pb and Cd resistance (IC50) and heteroresistance. Heteroresistance indicated variation in metal resistance between individual cells of clonal populations. Some isolates were also tested with Zn. The genomes of the isolates were sequenced and sequence data submitted to the NCBI/EBI.
Publication date: 2019-10-10
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Format

Comma-separated values (CSV)

Spatial information

Study area
Spatial representation type
Tabular (text)
Spatial reference system
OSGB 1936 / British National Grid

Provenance & quality

Soil-core samples were collected during July 2014 near the disused St. Anthony’s metal smelting works, at either contaminated (16.03 g kg-1 Pb, 10.87 mg Kg-1 Cd) or nearby less-contaminated (4.38 g kg-1 Pb, 1.49 mg Kg-1 Cd) areas. Coordinates and a description of the site are available at http://www.twsitelines.info/SMR/4192. Soil-core samples were diluted in the laboratory and spread to agar media for yeast isolation. Single colonies were subcultured and yeasts identified with ITS sequencing and RAPD PCR. 56 clonal isolates of Saitozyma podzolica were assayed for lead (Pb) and cadmium (Cd) resistance (IC50) and heteroresistance. Some isolates were also tested with zinc (Zn) Genomic DNA preparations submitted to NBAF-Liverpool for whole-genome sequencing. The sequence data were deposited with EBI/NCBI.

Licensing and constraints

This dataset is available under the terms of the Open Government Licence

Cite this dataset as:
Hewitt, S.; Warry, A.; Dyer, P.S.; Avery, S. (2019). Heterogeneity and environmental stress resistance of wild yeast isolates. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/

Correspondence/contact details

Simon Avery
University of Nottingham
Nottingham
UNITED KINGDOM
 enquiries@ceh.ac.uk

Authors

Hewitt, S.
University of Nottingham
Warry, A.
University of Nottingham
Dyer, P.S.
University of Nottingham
Avery, S.
University of Nottingham

Other contacts

Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
environment
INSPIRE theme
Environmental Monitoring Facilities
Keywords
Environmental stress resistance , Heterogeneity , Wild Yeast
Funding
Natural Environment Research Council
Last updated
08 February 2024 17:25