Hewitt, S.; Warry, A.; Dyer, P.S.; Avery, S.V.

Heterogeneity and environmental stress resistance of wild yeast isolates

This dataset contains analytical results for wild yeast isolates from soil samples at and near a disused metal smelting works in the north-east of the UK. The main contaminant from the smelting works was lead (Pb), but Cd was also elevated. The soil near the smelting works showed above-background levels of Pb. Yeasts of similar colony morphology on laboratory agar were isolated and proved to be mostly isolates of Saitozyma podzolica: 56 independent clonal isolates of this yeast were confirmed by ITS sequencing and RAPD-PCR. All of the S. podzolica isolates were tested for Pb and Cd resistance (IC50) and heteroresistance. Heteroresistance indicated variation in metal resistance between individual cells of clonal populations. Some isolates were also tested with Zn.
The genomes of the isolates were sequenced and sequence data submitted to the NCBI/EBI.

Publication date: 2019-10-10

Get the data

This dataset will be available under the terms of the Open Government Licence

Format of the dataset : Comma-separated values (CSV)

You must cite: Hewitt, S.; Warry, A.; Dyer, P.S.; Avery, S.V. (2019). Heterogeneity and environmental stress resistance of wild yeast isolates. NERC Environmental Information Data Centre. https://doi.org/10.5285/299f89cd-7bbe-4428-9552-c51981c5f883

 

© Natural Environment Research Council

Where/When

Study area
Temporal extent
2014-07-01    to    2014-07-31

Provenance & quality

Soil-core samples were collected during July 2014 near the disused St. Anthony’s metal smelting works, at either contaminated (16.03 g kg-1 Pb, 10.87 mg Kg-1 Cd) or nearby less-contaminated (4.38 g kg-1 Pb, 1.49 mg Kg-1 Cd) areas. Coordinates and a description of the site are available at http://www.twsitelines.info/SMR/4192. Soil-core samples were diluted in the laboratory and spread to agar media for yeast isolation. Single colonies were subcultured and yeasts identified with ITS sequencing and RAPD PCR. 56 clonal isolates of Saitozyma podzolica were assayed for lead (Pb) and cadmium (Cd) resistance (IC50) and heteroresistance. Some isolates were also tested with zinc (Zn) Genomic DNA preparations submitted to NBAF-Liverpool for whole-genome sequencing. The sequence data were deposited with EBI/NCBI.

Supplemental information

Other useful information regarding this dataset:

St. Anthony’s metal smelting works

Correspondence/contact details

Simon Avery
University of Nottingham
 Simon.Avery@nottingham.ac.uk

Authors

Hewitt, S.
University of Nottingham
Warry, A.
University of Nottingham
Dyer, P.S.
University of Nottingham
Avery, S.V.
University of Nottingham

Other contacts

Custodian
Environmental Information Data Centre
 eidc@ceh.ac.uk
Publisher
NERC Environmental Information Data Centre
 eidc@ceh.ac.uk

Additional metadata

Topic categories
Biota
Keywords
Environmental stress resistance,  Heterogeneity,  Wild Yeast
INSPIRE Theme
Soil
Funding
Natural Environment Research Council
Spatial representation type
Tabular (text)
Spatial reference system
WGS 84
Last updated
2019-10-11