These data are suspended organic matter stocks of coarse and fine particulate organic matter in eight Welsh upland rivers with contrasting land-use, moorland and exotic conifer, in response to riparian deciduous leaf addition. Eight sampling reaches were chosen at two sites, Llyn Brianne (4 reaches) and Plynlimon (4 reaches). The experiment consisted of adding deciduous leaves to half of the reaches whilst the other half were maintained as a control (no addition of deciduous leaves). To characterise the suspended organic matter of the studied streams, water was filtered to collect monthly samples during from December 2012 and January 2013 (before deciduous leaf addition) and from February to April 2013 (after deciduous leaf addition) in each sampling reach.
The main goal of this survey was to examine how aquatic biodiversity and organic matter stocks respond to leaf addition in moorland and conifer forested rivers. Dr Isabelle Durance was responsible for organising the surveys, Marian Pye was in charge of collecting, processing and sorting the samples. The work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was a project funded by the Natural Environment Research Council (NERC) Biodiversity and Ecosystem Service Sustainability (BESS) programme.
Publication date: 2017-04-20
This dataset is part of the following
Each stream was divided into an upstream control zone and a downstream experimental zone. Samples of suspended organic matter were collected by filtering water. Deciduous leaves were added directly to the stream and by fixing 'Onion bags' of leaves the stream bed and bank. Samples were collected on six occasions, December (3-10/12/2012) January (3-16/01/2013), February (4-7/02/2013), March (11-14/03/2013), April (3-8/04/2013) and May (22-26/05/2013) in all the streams in both the upstream reference zones and downstream experimental zones. Upon collection,Suspended Organic Matter (SOM) samples were refrigerated at 4 degrees celsius, returned to the laboratory and frozen within 24 hours. Frozen SOM samples were then freeze-dried at -20 degrees Celsius for 48 to 72 hours and weighed to the nearest 0.0001gramme. Freeze-dried SOM samples were ground, homogenised and subsampled (3mg ±0.3mg) for analysis of elemental (C, N) and stable isotopic (delta-13C, delta-15N) composition on a mass spectrometer (University of California Davis Stable Isotope Facility). All SOM samples were corrected for inorganic content by combusting a subset (%) of samples from each site at 550 degrees Celsius for 5 hours and applying an ash-free conversion factor. Results were recorded in an Excel spreadsheet. Data was exported to a comma separated value files for ingestion into the Environmental Information Data Centre (EIDC).