Hellmann, E.; Friend, A.D.
Growth of poplar seedlings grown in chambers following variations in photoperiod and temperature
This dataset is under embargo and will be made available by 1 January 2026 at the latest Find out more »
Cite this dataset as:
Hellmann, E.; Friend, A.D. (2025). Growth of poplar seedlings grown in chambers following variations in photoperiod and temperature. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/46c23be6-63ba-400d-859b-050dcb207898
Download/Access
This dataset is under embargo and will be made available by 1 January 2026 at the latest Find out more »
https://doi.org/10.5285/46c23be6-63ba-400d-859b-050dcb207898
This dataset contains information on how variations on temperature and photoperiod affects poplar seedling growth in Eco chambers. Plants were propagated on sterile media (1/2MS, 0.7% Agar, 1mg/L IAA) by cutting the shoot tip and 3-4 leaves and placing them in a weck jar on new medium. Once the explants rooted after incubation at 200 mmol light, 21C day/18C night, long day conditions (16:8) and ambient humidity, they were transplanted onto soil (6:2:1 Peat-free: sand-vermiculite, fertilizer: 4 g/L Osmocote exact standard 15-9-12) and grown first under protective covers and then open in 24C, long day conditions (LD), 65% humidity, 350mmol light (experiments R1-3) or transferred into the Eco chamber (temperature as indicated in experiment, 80-98% humidity, 350mmol LED light, long day conditions). After 3-4 weeks, plants of R1-R3 were transferred in the conditions described in the experiment (R1: 24C SD vs 15C LD; R2: 15C SD vs 15C LD; R3: 15C LD vs 24C LD) and grown for a month before moving them into 5C low light conditions which resulted in senescence and leaf drop. Eco chamber plants were grown in the Eco chamber for about four weeks and after not showing senescence, harvested directly after the experiment without cold incubation.
All plants were measured in height and diameter (6cm above soil), leaf number and area regularly and gas exchanged was measured with a TARGAS-1 instrument (Hansatech) at 500mmol light intensity. After the experiment, cross-sections were taken and embedded in resin for further microscopic analysis (data not ready and not included).
All plants were measured in height and diameter (6cm above soil), leaf number and area regularly and gas exchanged was measured with a TARGAS-1 instrument (Hansatech) at 500mmol light intensity. After the experiment, cross-sections were taken and embedded in resin for further microscopic analysis (data not ready and not included).
Publication date: 2025-04-17
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Format
Comma-separated values (CSV)
Temporal information
Temporal extent
2023-01-01 to 2023-12-31
Provenance & quality
The data have been generated using calipers, a laser distance meter, Image J for leaf area and a TARGAS-1 instrument for photosynthetic gas exchange. The data have been averaged between 20-25 plants and the standard deviation calculated.
Licensing and constraints
This dataset is under embargo and will be made available by 1 January 2026 at the latest Find out more »
This dataset will be available under the terms of the Open Government Licence
Cite this dataset as:
Hellmann, E.; Friend, A.D. (2025). Growth of poplar seedlings grown in chambers following variations in photoperiod and temperature. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/46c23be6-63ba-400d-859b-050dcb207898
Correspondence/contact details
Hellmann, E.
Department of Geography, Cambridge, The Sainsbury Laboratory, Cambrigde
Eva.hellmann@slcu.cam.ac.uk
Authors
Hellmann, E.
Department of Geography, Cambridge, The Sainsbury Laboratory, Cambrigde
Other contacts
Rights holder
University of Cambridge
Custodian
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk