Cite this dataset as
Blount, J.D.; Cant, M.A.; Meniri, M.; Thompson, F.J.; Marshall, H.H. (2022). Oxidative stress markers in banded mongooses in Queen Elizabeth National Park, Uganda, 2017-2021. NERC EDS Environmental Information Data Centre. (Dataset). https://doi.org/10.5285/481c424e-f277-4eb7-9ee0-cbdb5532ef79
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This dataset is available under the terms of the Open Government Licence
Comma-separated values (CSV)
Individuals were blood sampled from the jugular vein under general anaesthesia. Samples were collected into EDTA-coated Vacutainers before plasma and erythrocytes were separated by centrifugation then snap frozen in liquid nitrogen within 10 minutes of collection. Samples were shipped to the UK within 3 months under cryogenic conditions, and stored at -80C until analysis.
Analyses were performed blindly with respect to sample identity, and all steps were conducted on ice. All chemicals were HPLC grade, and chemical solutions were prepared using Milli-Q water. Assays were conducted within one year of collection.
Malondialdehyde was determined by HPLC with fluorescence detection (Agilent 1000). Protein carbonyls, glutathione and superoxide dismutase were determined using colorimetric assay kits (Cayman Chemical Company) and a Molecular Devices plate reader (Spectramax M2).
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This dataset is available under the terms of the Open Government Licence
CITE AS: Blount, J.D.; Cant, M.A.; Meniri, M.; Thompson, F.J.; Marshall, H.H. (2022). Oxidative stress markers in banded mongooses in Queen Elizabeth National Park, Uganda, 2017-2021. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/481c424e-f277-4eb7-9ee0-cbdb5532ef79