Features detected by untargeted metabolomic analysis of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA
Cite this dataset as:
Connolly, J. A.; Del Carratore, F.; Schmidt, K.; Bisesi, A.; Martinson, J. N. V.; Chua, J.; Kuhs, M.; Boneza, M.; Heinsch, S.; Kinkel, L.; Smanski, M.; Harcombe, W. R.; Breitling, R.; Takano, E. (2024). Features detected by untargeted metabolomic analysis of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/4921f306-5efa-4bf7-a15e-a157c2665889
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This dataset is available under the terms of the Open Government Licence 
https://doi.org/10.5285/4921f306-5efa-4bf7-a15e-a157c2665889
This dataset contains features detected by untargeted metabolomics analysis of paired cultures of 4 interacting Streptomyces bacteria. The bacteria were originally isolated from a nature reserve in Minnesota USA. Daily samples of spot cultures grown in adjacent pairs on solid ISP2 medium were taken from day 2 to 6 in triplicate. Metabolites were obtained by cold methanol extraction, and liquid chromatography-mass spectrometry data was collected in both positive and negative ionisation modes. This dataset contains the mass-to-charge (m/z) and retention time of identified peaks, and their ion intensity in each sample. The relative abundance of these identified features was later compared between pairs of interacting strains to gain insight into changes in their metabolism.
This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458).
This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458).
Publication date: 2024-11-21
View numbers valid from 21 November 2024 Download numbers valid from 21 November 2024 (information prior to this was not collected)
Format
Comma-separated values (CSV)
Provenance & quality
The 4 Streptomyces strains were previously isolated from Minnesota soil as described in Schlatter, D.C. et al (2013), co-culturing, metabolite extraction and LC-MS experiments were performed in the Manchester Institute of Biotechnology, University of Manchester, UK. Data was collected on a Q-Exactive Mass Spectrometer (ThermoFisher). Outputs were converted to mzML format by the ProteoWizard MS converter. Noise removal, signal filtering and peak matching was performed in the open source mzMatch software. Pooled quality control samples were analysed at every 6th injection to assess for analytical drift over time. The sample injection sequence was randomised.
Licensing and constraints
This dataset is available under the terms of the Open Government Licence
Cite this dataset as:
Connolly, J. A.; Del Carratore, F.; Schmidt, K.; Bisesi, A.; Martinson, J. N. V.; Chua, J.; Kuhs, M.; Boneza, M.; Heinsch, S.; Kinkel, L.; Smanski, M.; Harcombe, W. R.; Breitling, R.; Takano, E. (2024). Features detected by untargeted metabolomic analysis of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/4921f306-5efa-4bf7-a15e-a157c2665889
Related
Correspondence/contact details
Authors
https://orcid.org/0000-0003-3643-5578
Schmidt, K.
University of Manchester
Bisesi, A.
University of Minnesota
Martinson, J. N. V.
University of Minnesota
Chua, J.
University of Manchester
Kuhs, M.
University of Minnesota
Boneza, M.
University of Minnesota
Heinsch, S.
University of Minnesota
Smanski, M.
University of Minnesota
Harcombe, W. R.
University of Minnesota
Other contacts
Rights holder
University of Manchester
Custodian
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk