Glanville, H.C.; De Sosa, L.L. ; Marshall, M.R.; Cooper, D.M.; Jones, D.L.

Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a high molecular weight substrate added as a carbon source, Conwy catchment, North Wales, UK (2016)

Time series data of carbon release in disintegrations per minute are presented for different nitrogen and phosphorus treatments with a high molecular weight substrate added as a carbon source to soil samples from six depths (0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres). Soil cores were collected from a field experiment in the Conwy catchment in July 2016 and returned the laboratories of the School of Environment, Natural Resources and Geography, Bangor University. A high molecular weight substrate was added as a carbon source to the samples and the rate of 14C-substrate mineralization measured.
All the work was carried out by trained members of staff from Bangor University and the Centre for Ecology and Hydrology.
The measurements were taken to improve understanding of the relationship between microbial activity and soil properties and depth of sampling, under differing nutrient availability.
The data were collected for the NERC project 'The Multi-Scale Response of Water quality, Biodiversity and Carbon Sequestration to Coupled Macronutrient Cycling from Source to Sea' (NE/J011991/1). The project is also referred to as Turf2Surf.


Publication date: 2018-03-20

Access and use conditions

This resource is embargoed and will be made available by 31 July 2018 at the latest  

This resource will be available under the terms of the Open Government Licence

You must cite: Glanville, H.C.; De Sosa, L.L. ; Marshall, M.R.; Cooper, D.M.; Jones, D.L. (2018). Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a high molecular weight substrate added as a carbon source, Conwy catchment, North Wales, UK (2016). NERC Environmental Information Data Centre. https://doi.org/10.5285/4a8fedf4-a572-4d95-ac41-087974169af1

 

Where/When

Study area
Temporal extent
2016-07-15    to    2016-09-30

Supplemental information

Other useful information regarding this dataset:

Project website

Quality

Lineage
Soil cores were collected from the field site and returned to laboratories of the School of Environment, Natural Resources and Geography, Bangor University.
In the laboratory soil cores were divided into depth intervals of 0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres and passed through a 5 millimetre sieve in order to remove stones and any plant material and to ensure sample homogeneity.
To measure the rate of 14C-substrate mineralization, 5 grams of soil (dry weight equivalent to account for soil water content variability down the soil profile) was placed into sterile 50 millilitre (ml) polypropylene tubes. To determine the rate of 14CO2 evolution 160 microlitres (µl) of the recalcitrant 14C-DOC labelled nutrient solution was added to the soil surface. Immediately after nutrient addition, a vial containing 1 millilitre (ml) Sodium Hydroxide (NaOH) trap (1 Molar (M)) was added into the polypropylene tubes to capture 14CO2 evolved. The tubes were hermetically sealed and incubated at 10 degrees Celsius to represent the mean annual temperature of the catchment. The NaOH traps were changed at 1, 6, 24, 48, 72, 168, 336, 504, 672, 840, 1176, 1512 and 1680 hours. On removal, the NaOH traps were mixed with Optiphase HiSafe 3® liquid scintillation fluid (PerkinElmer Inc.) and the amount of 14CO2 captured was determined using a Wallac 1404 liquid scintillation counter (Wallac EG & G).
Data were entered into an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC.

Authors

Glanville, H.C.
Bangor University
De Sosa, L.L.
Bangor University
Marshall, M.R.
Centre for Ecology & Hydrology
Cooper, D.M.
Centre for Ecology & Hydrology
Jones, D.L.
Bangor University

Other contacts

Custodian
Environmental Information Data Centre
Publisher
NERC Environmental Information Data Centre
Point Of Contact
Glanville, H.C.
Bangor University
Distributor
Environmental Information Data Centre

Spatial

Spatial representation type
Tabular (text)
Spatial reference system
WGS 84 longitude-latitude (CRS:84)

Keywords

Topic categories
Environment
Other keywords
Soil Core
CEH Topic
Environmental survey
INSPIRE Theme
Environmental Monitoring Facilities
Instrument keywords
liquid scintillation counter
Place keywords
North Wales
Conwy catchment
Project keywords
T2S
Turf2Surf
Macronutrient Cycling Programme
MCP

Dataset identifiers

https://catalogue.ceh.ac.uk/id/4a8fedf4-a572-4d95-ac41-087974169af1
doi:10.5285/4a8fedf4-a572-4d95-ac41-087974169af1

Metadata

Information maintained by
Cooper, D. M.
Centre for Ecology & Hydrology
Environmental Information Data Centre
Last updated
2018-03-20T16:34:12